Welcome to the Wisconsin Crop Innovation Center Panelists: Mike - - PowerPoint PPT Presentation

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Nov 02, 2022 215 likes •552 views

Welcome to the Wisconsin Crop Innovation Center Panelists: Mike Petersen Associate Director, WCIC Dr. Shawn Kaeppler Campbell Bascom Professor of Agronomy and Director, WCIC Dr. Heidi Kaeppler Associate Professor,

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Welcome to the Wisconsin Crop Innovation Center

Panelists:

Mike Petersen ‐ Associate Director, WCIC
Dr. Shawn Kaeppler – Campbell‐Bascom Professor of Agronomy and Director, WCIC
Dr. Heidi Kaeppler – Associate Professor, Agronomy
Andrew Bent – Professor, Plant Pathology

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History of the Wisconsin Crop Innovation Center

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1981‐1984

Cetus of Madison, Inc. opens with

UW Bacteriology Professor Winston Brill as Director and WARF funding

Research focus was on isolating and

characterizing Rhizobium species for better nitrogen fixation in soybean

WR Grace Co. purchases 51% equity

and name changes to Agracetus

Research focus changes to plant

transformation research and development

1981 1982 1984

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1985‐1990

Agracetus scientists invent the

electric “Gene Gun” and begin to successfully transform many different plant species including tobacco, peanut, sunflower, soybean, maize, cotton, canola, poplar, wheat, rice, among others

1986 – 1st field release of a GMO in the

US (herbicide resistance in tobacco)

Transformation services contracted out

to private companies (Monsanto, etc.)

Electric Gene Gun‐1986 Tobacco

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1991‐1995

Agracetus research continues in plant

transformation and improving their gene gun techniques

DNA vaccine work initiated with a newly

designed handheld gene gun

Contract work with Monsanto continues

and leads to the Round‐up Ready Soybean event that is taken to market in 1996 as the first large scale, commercially successful transgenic plant sold on millions of acres across the US

Handheld Gene Gun

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1996‐2016

Monsanto purchases Agracetus
Greenhouse and laboratory space is expanded
Research changes focus to Agrobacterium

transformation and high throughput automation

f the process
Site becomes the exclusive facility for all

soybean, cotton, and canola transformation for Monsanto

Center of excellence for R&D activities including

corn, wheat, rice, and alfalfa transformation, automation, gene expression, molecular testing, and seed chipping

In 2015, due to business consolidation,

Monsanto generously donates the facility and much equipment to the UW‐Madison

Seed Chipping GFP Gene Expression

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2017

Wisconsin Crop Innovation Center

(WCIC) is born

Plant transformation and editing
Phenotyping
Research collaborations
Teaching/Education
Research & Development
Outreach

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Mission, Business Model, Potential

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Mission

The mission of WCIC is to advance basic and applied translational

and functional genomic research in crop plants through technology development, collaboration, and fee‐for‐service transformation, gene‐editing, and phenotyping activities.

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Business Model

Fee‐for‐service and collaborations
Grants for technology development
Partner with WARF on IP protection
Education and Outreach
Facility Use Agreements to support partnership with local business
e.g Middleton Spectral

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WCIC will enable UW and U.S. public research

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WCIC will enable Technology Transfer

Technology Transfer Valley‐

f‐Death

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Thank you. Questions?

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WCIC Genetic Engineering and Gene Editing Technologies for Crop Genomics Research and Genetic Improvement

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Crop genetic engineering/editing applications

Study plant gene

function and regulation

Introduce novel, useful

traits

Improve current traits
Turn off negative traits

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Examples of current and potential traits of engineered/edited crops

Enhanced yield of food, feed, fiber, biofuels with less

inputs and reduced ecological footprint

Built-in disease, pest and stress resistance
Built-in herbicide resistance
Enhanced nutritional value or removal of

“antinutritional or toxic compounds”

Reduced allergenicity
“Green” pharmaceutical production

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Genome editing system for crop plants

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Mechanisms of Resistance to Soybean Cyst Nematodes

Andrew Bent University of Wisconsin - Madison

TumblingRun / Flickr

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Soybean cyst nematodes in soybean root (stained red with acid fuchsin)

Photo: University of Missouri Extension

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Widespread in U.S.,

Brazil, China

Inoculum pool only

hatches gradually over a decade

Photo: Kris Lambert

Soybean Cyst Nematode (SCN) is the most economically damaging pathogen or pest of soybean

http://extension.entm.purdue.edu

SLIDE 23

Soybean Cyst Nematode Distribution 1957 - 2014

Figures adapted from “ Increased Reproduction of Soybean Cyst Nematode and Yield Of SCN-Resistant Soybean Varieties” – Greg Tylka, Iowa State

1957 2014 2001 1990 1980 1973

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Syncytium: Biotrophic Interface for 3-4 Weeks

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Rhg1 is a ~30kb block containing 4 ORFs: 2580, 2590, 2600 and 2610 Susceptible Varieties: Rhg1 block present in one copy Resistant Varieties: Rhg1 block present in multiple tandem repeat copies 31.2 kb Three of the gene products contribute to resistance

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SCN Resistance: Slow Erosion

PI 88788

Zheng and Chen. Journal of Ent. and Nem. 2011

Partial breakdown of PI88788 resistance

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Katelyn J. Horgan | Ph.D. Candidate

UC Davis

Towards the identification of novel soybean cyst nematode resistance genes

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Stacking G. soja QTL

L. Brzostowski & B. Diers, unpublished

cqSCN006 cqSCN007 Rhg1 Chr 10 No QTL Rhg1 + cqSCN006 + cqSCN007 + Chr 10

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cqSCN007

146 kb

Chr. 18

1 1

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CRISPR/Cas9 to mutate targeted genes

1 2

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Funding: United Soybean Board USDA-NIFA APS/Pioneer & NSF Fellowships