Vronique Decroocq INRA Bordeaux The sharka disease, a major - - PowerPoint PPT Presentation

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Vronique Decroocq INRA Bordeaux The sharka disease, a major - - PowerPoint PPT Presentation

Vronique Decroocq INRA Bordeaux The sharka disease, a major phytosanitary problem in stone fruit production Plum, peach, apricot, cherry, almond Due to Plum Pox Virus (PPV) infection, a quarantine pathogen Transmissible by aphids and


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Véronique Decroocq

INRA Bordeaux

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The sharka disease, a major phytosanitary problem in stone fruit production

  • Due to Plum Pox Virus (PPV) infection, a quarantine

pathogen

  • Transmissible by aphids and grafting
  • Major socio-economic impact, especially in

Eastern and Southern EU impoverished regions Plum, peach, apricot, cherry, almond

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The routes to sharka dissemination

NURSERIES ORCHARDS

Grafting Aphids

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Sharka propagation EU- and world-wide

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Sharka propagation EU- and world-wide

North America 1999 (PPV-D) Bulgaria 1917-1918 Chile 1993 (PPV-D) Japan 2009 China 2004 India 1994 Egypt 1988

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Impact of sharka on fruit production

  • Disease management EU-wide 10 billions € / 30 y
  • Loss in apricot production 3.5 billions €
  • EU entire regions of apricot or peach production dropped cultivation because
  • f heavy sharka infection (Spain -Valenciano-, Greece –Peloponese-,

Austria…) NURSERIES ORCHARDS

  • Loss of phytosanitary passport for three

consecutive years, at least. Sale and production

  • f Stone fruit plants are completely forbidden.
  • Infected trees are eliminated.

If more than 10% of the

  • rchard is infected, the field

is removed.

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Methods of sharka containment

  • Plantation of healthy material (certified virus-free)
  • Control of aphids (but not efficient in the case of sharka)
  • Eradication of any PPV infected wild or cultivated trees around

nurseries and orchards

  • Development of sharka resistant varieties
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Methods of sharka containment

  • Development of sharka resistant varieties

Resistance Prunus davidiana Prunus dulcis (almond) Susceptibility No peach genitor resistant to sharka Hypersensitivity ‘Jojo’ Susceptibility One single source of resistance in plum Tolerance Partial resistance Resistance Immunity ‘Goldrich’ ‘Henderson’ ‘SEO’ ‘Stella’ ‘Harlayne’ ‘NJA2’ ‘Harcot’ Susceptibility Few Apricot genitors of the same genetic origin

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From SharCo to MARS

SharCo for Sharka Containement (2008-2012): Three main research focus

1) Epidemiology 2) Genetic 3) Guidelines for disease management

  • Highthroughput selection of PPV resistant, new apricot

cultivars, adapted to the EU-wide cultivation requirement (from North to South, from West to East)

  • Transfer of the technology and the plant material to SMEs

MARS for Marker Assisted Resistance to Sharka

(2013-2015)

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France Spain Germany Poland Czech R Slovakia Romania Serbia Bulgaria Turkey Italy

17 partners: 9 public institutions INRA (France) UMIL (Italy) UNIBO (Italy) USAMV (Romania) FGI (Bulgaria) MKU (Turkey) NAGREF (Greece) CEBAS-CSIC (Spain) Mendelu Univ (Czech rep) 8 private partners ADNid (France) Beta Company (Turkey) Food Allergens (Greece) CEP Innovation (France) C.R.P.V (Italy) Juglans regia (Bulgaria) EFE VIVEROS del Sureste (Spain) Phytoria Karayiannis (Greece)

MARS: For EU-wide MAS of PPV resistant apricot (2013-2015)

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Expected results of MARS: Marker Assisted Resistance to Sharka

  • To

provide highly competitive trees AND molecular tools for breeding for resistance to PPV and ensuring the safe production of stone fruits

  • To

transfer knowledge and technologies to SMEs in order to speed up in Europe the selection of innovative plant material resistant to Plum pox virus

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MARS activities and achievements

  • The Marker Assisted Selection technology: Legacy of the

SharCo FP7 project Filippo Geuna and Daniele Bassi (UNIMIL)

  • The MARS outcomes for the apricot fruit industry

Fabienne Moreau (ADNid) and Manuel Rubio (CEBAS-CSIC)