Use of Fluoroquinolones and Development of Resistance Pascal - - PowerPoint PPT Presentation

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use of fluoroquinolones and development of resistance
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Use of Fluoroquinolones and Development of Resistance Pascal - - PowerPoint PPT Presentation

Oct 20, 2022 354 likes •448 views

Focus Group Meeting on Fluoroquinolones: Use of Fluoroquinolones and Development of Resistance Pascal Sanders, SAGAM member AFSSA, France Proportion resistance (%) to quinolones among Salmonella and Campylobacter from food-producing animals

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Focus Group Meeting on Fluoroquinolones:

Use of Fluoroquinolones and Development of Resistance

Pascal Sanders, SAGAM member AFSSA, France

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Proportion resistance (%) to quinolones among Salmonella and Campylobacter from food-producing animals

(Data from ARBAO II, Anon. 2004) European Medicines Agency (EMEA), London, UK www.emea.europa.eu

12 5 26 Switzerland 2 18 Sweden 8 Poland Norway 38 11 3 3 Netherlands 44 Greece 8 14 4 Germany 36 20 France 17 8 England and Wales 11 8 23 <1 Denmark 6 Belgium 2 Austria Poultry Cattle Pigs

  • S. Enteritidis

poultry

  • S. Typhimurium

pigs

  • C. jejuni
  • C. coli

Salmonella Pathogen and percent reported resistance to quinolones Country

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Proportion resistance (%) to FQs and flumequine or nalidixic acid among Escherichia coli from pigs and broilers sampled at slaughter

Sources: Austria (Steiermark): REMOST, France: AFSSA, Denmark: DANMAP 2003, Finland: FINRES, Italy: ITAVARM, The Netherlands: MARAN 2003, Norway: NORM/NORMVET, Sweden: SVARM 2003.

5 1 35 50 28 2 10 54 >16 or >4 Nalidixic acid

  • r flumequine

3 11 3 3 >2 FQb, (n=306) (n=141) (n=165) (n= 258) (n=308) (n=300) (n=138) (n=140) Broilers 1 <1 9 3

  • 2

8 >16 or >4 Nalidixic acid

  • r flumequine

<1

  • 2

>2 FQb, (n=303) (n=187) (n=155) (n=255) (n=304)

  • (n=317)

(n=134) Pigs Sweden Norway Netherlands Italy France Finland Denmark Austria Country and percent resistance Break- point (mg/l) Origin and quinolone

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Proportion resistance (%) to fluoroquinolones among animal pathogens from food-producing animals *

* data from ARBAO II (Anon. 2004) < 1 3 1 Sweden 16 12 Spain 76 Portugal 1 Poland 2 Norway 2-6 0-1 Netherlands 44 Latvia 4 22 Italy 2 1 2 Germany 5 5 5 5 31 France 11 Finland 8 3 < 1 4 England and Wales 1 Denmark 3 35 Belgium Cattle Cattle Pigs Poultry Cattle Pigs M. haemolytica

  • P. multocida
  • E. coli
  • A. pleuro-

pneumoniae Country

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  • Mechanism of action

– Inhibition of DNA gyrase activity – Concentration-dependent drugs – Bactericidal : rapid reduction of susceptible bacterial population

  • Pharmacokinetics

– Good oral bioavailability of FQs – Excretion as active drugs in intestinal lumen

Selection for FQ resistance

European Medicines Agency (EMEA), London, UK www.emea.europa.eu

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Mechanisms of resistance

European Medicines Agency (EMEA), London, UK www.emea.europa.eu

2nd

ParE, ParC

1st

GyrA, GyrB

Mutation

Lack of topoisomerase IV Topoisomerase IV (F)Quinolone R DNA gyrase Quinolone R (F)Quinolone R DNA gyrase Quinolone R Reduced (F)Q susceptibility

Campylobacter Enterobacteriaceae

+ Decreased uptake or efflux – Not specific + qnr, plasmid mediated gene

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Monitoring of resistance Epidemiological cut-off value

European Medicines Agency (EMEA), London, UK www.emea.europa.eu

Campylobacter

Ciprofloxacin WT ≤1 µg/ml Nalidixic acid WT ≤16 µg/ml

Salmonella

Ciprofloxacin WT ≤0.064 µg/ml Nalidixic acid WT ≤16 µg/ml

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Thank you! Thank you!