SLIDE 1
HBV
Testing for drug resistance and resistance interpretation
Martin Däumer Institute of Immunology and Genetics Kaiserslautern
HBV Testing for drug resistance and resistance interpretation - - PowerPoint PPT Presentation
HBV Testing for drug resistance and resistance interpretation - - PowerPoint PPT Presentation
HBV Testing for drug resistance and resistance interpretation Martin Dumer Institute of Immunology and Genetics Kaiserslautern Genome of HBV HBV drug resistance testing technical approach 0.25 Product 1 (base 377 to 864) For: 5
SLIDE 2
SLIDE 3
- N236T
- 0.25
- 0.87
Product 1 (base 377 to 864) For: 5’GGATGTGTCTGCGGCGTTT3’ Rev: 5’ACCCCATCTTTTTGTTTTGTTAGG3’ Product 2 (base 252 to 819) For: 5’AGACTCGTGGTGGACTTCTCT3’ Rev: 5’CAAAAGAAAATTGGTAACAGCGGTA3’
Allen et al., 1998
HBV drug resistance testing – technical approach
SLIDE 4
Editing: Seqman (Lasergene)
SLIDE 5
HBV Resistance Mutations
845 a.a.
Terminal protein Spacer Pol/ RT RNaseH
A B C E D YMDD GVGLSPFLLA I(G) II(F)
Allen MI, et al. Hepatology. 1998;27:1670-1677. Qi X, et al. J Hepatol. 2004;40(suppl 1):20- 21. Tenney D, et al. Antimicrob Agents Chemother. 2004;48:3498-3507. Tyzeka [package insert]. Lai CL, et al. Gastroenterology. 2005;129:528-536. Schildgen O, et al. N Engl J Med. 2006;354:1807-1812. Locarnini S. 2006 IDRW. Abstract P2.
rtL80V/ I rtV173L rtL180M rtA181T/ V rtN236T rtl233V ? rtM204V/ I rtS202G/ C rtT184S/ A/ I / L ETV resistance rtL180M rtM204I LdT resistance rtL80V/ I rtL180M TDF resistance rtA194T rtM204V rtL180M rtM204V/ I / S LAM resistance ADV resistance rtM250I / V
SLIDE 6
HBV genotypes- worldwide distribution
- Schaefer, J Viral Hep 2005
SLIDE 7
E D G A B
HBV genotypes - Cologne data
■genotype D is predominating
SLIDE 8
Genafor/ Arevir HBV drug resistance interpretation tool [genafor.org]
SLIDE 9
Genafor/ Arevir HBV drug resistance interpretation tool
SLIDE 10
Genafor/ Arevir HBV drug resistance interpretation tool
SLIDE 11
SLIDE 12
SLIDE 13
SLIDE 14
- Joachim Selbig,
MPI für Molekulare Pflanzenphysiologie Golm und Universität Potsdam Joachim Büch, Andre Altmann, Thomas Lengauer, MPI für Informatik Saarbrücken Rolf Kaiser, Nadine Sichtig, Saleta Sierra Aragon, Melanie Balduin, Eugen Schülter, Herbert Pfister, Institut für Virologie, Gerd Fätkenheuer, Innere Medizin I, Universitätsklinik zu Köln Ulrike Schuldenzucker Europa Fachhochschule Fresenius, Köln Klaus Korn, Barbara Schmidt, Hauke Walter, Nationales Referenzzentrum für Retroviren, Universität Erlangen-Nürnberg Patrick Braun, Robert Ehret, Heribert Knechten Praxiszentrum Blondelstraße, Aachen Bernd Kupfer, Bertfried Matz, Karl E. Schneweis, Med. Mikrobiologie Jürgen K. Rockstroh, Medizinische Klinik I, Universitätsklinik Bonn Mark Oette, Claudia Müller, Klinik f. Gastroenterologie, Hepatologie u. Infektiologie, Universitätsklinikum Düsseldorf