UMCES INTERNSHIP Summer 2019 - Scott McKinstry WORKING IN A LAB - - PowerPoint PPT Presentation

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Feb 18, 2023 228 likes •422 views

UMCES INTERNSHIP Summer 2019 - Scott McKinstry WORKING IN A LAB Safety and appropriate lab wear. Understanding of materials and equipment used. Mindfulness of others in the lab. Communication about supplies and equipment.

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UMCES INTERNSHIP

Summer 2019 - Scott McKinstry

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WORKING IN A LAB

Safety and appropriate lab wear. Understanding of materials and equipment used. Mindfulness of others in the lab. Communication about supplies and equipment.

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CLEANLINESS & NEATNESS

YAY NAY

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OTHER OPPORTUNITIES

Bird Walks Ecology Readings AMJV Lab Events

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INTERNSHIP RESEARCH

Identification of sources of nitrate (NO3) in rivers during storm events. Isotopic composition differences between sources of NO3 measured using the denitrifier method and IRMS.

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WHY WE CARE

Excess NO3 pollution from upland watersheds contributes to algal blooms and the formation of dead zones in the Chesapeake Bay. (Kemp et al. 2005) Understanding the sources of NO3 can allow us to take steps to decrease the amount of nitrogen entering the Bay.

https://mde.maryland.gov/programs/Water/HA /Pages/index.aspx

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DENITRIFIER METHOD

 First, the bacteria are grown on shaker table

vernight.

 Next, the bacteria are mixed with a nitrate-free broth and injected into vials.  The vials are then placed on a “purging” rack, where helium is entered into the vials and any atmospheric gases (N2 and O2) are emitted.  Lastly, the samples collected from the storm events are added into the vials and bacteria convert NO3 to nitrous oxide (N2O).

Soto et al. 2015

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WATERSHEDS

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RESEARCH QUESTION

Does the size of the storm have a correlation with the concentration of total and atmospheric NO3?

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R² = 0.324 R² = 0.0642 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 0.00 1.00 2.00 3.00 4.00 5.00 6.00

vent Mean Concentration of NO3 (mg/L) Total Precipitation Depth (cm)

Total Precipitation Depth (cm) vs. Event Mean Concentration (mg/L)

GUN GWN

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TOTAL NO3 LOAD (KG/HA) VS. MAX PRECIPITATION INTENSITY(CM/HR.)

0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 2.00 4.00 6.00 8.00 Tot al N03 Load (kg/ha) Maximum Precipitation Intensity (cm/hr)

Gunpowder Falls

0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 5 10 15 20 Total NO3 Load (kg/ha) Maximum Precipitation Intensity (cm/hr)

Gwynn's Falls

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0.00 0.02 0.04 0.06 0.08 0.10 0.12 200 400 600 800 1000 1200 1400 1600 1800 12/28/2018 0:00 12/28/2018 4:48 12/28/2018 9:36 12/28/2018 14:2412/28/2018 19:12 12/29/2018 0:00 12/29/2018 4:48 12/29/2018 9:36

ATMOSPHERIC NO3 CONCENTRATION (MG/L) DISCHARGE (CFS) DATE & TIME

Gunpowder Falls - Storm Event 3

Discharge

Atm. NO3 Concentration

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0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 100 200 300 400 500 600 700 800 900 1000 5/5/2019 0:00 5/5/2019 4:48 5/5/2019 9:36 5/5/2019 14:24 5/5/2019 19:12 5/6/2019 0:00 5/6/2019 4:48

ATMOSPHERIC NO3 CONCENTRATION (MG/L) DISCHARGE (CFS) DATE & TIME

Gunpowder Falls – Storm Event 6

Discharge

Atm. NO3 Concentration

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0.02 0.04 0.06 0.08 0.1 0.12 0.14 100 200 300 400 500 600 700 800 900 1000

12/27/2018 19:12 12/28/2018 0:00 12/28/2018 4:48 12/28/2018 9:36 12/28/2018 14:24 12/28/2018 19:12 12/29/2018 0:00 12/29/2018 4:48 12/29/2018 9:36

ATMOSPHERIC NO3 CONCENTRATION (MG/L) DISCHARGE (CFS) DATE & TIME

Gwynn’s Falls – Storm Event 3

Discharge

Atm. NO3 Concentration

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0.02 0.04 0.06 0.08 0.1 0.12 0.14 200 400 600 800 1000 1200 1400 1600 1800 3/21/2019 4:48 3/21/2019 9:36 3/21/2019 14:24 3/21/2019 19:12 3/22/2019 0:00 3/22/2019 4:48 3/22/2019 9:36

Atmospheric NO3 Concentration (mg/L) Discharge (cfs) Date & Time

Gwynn’s Falls – Storm Event 5

Discharge

Atm. NO3 Concentration

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0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.2 100 200 300 400 500 600 700 800 5/4/2019 19:12 5/5/2019 0:00 5/5/2019 4:48 5/5/2019 9:36 5/5/2019 14:24 5/5/2019 19:12 5/6/2019 0:00 5/6/2019 4:48 5/6/2019 9:36

ATMOSPHERIC NO3 CONCENTRATION (MG/L) DISCHARGE (CFS) DATE & TIME

Gwynn's Falls - Storm Event 6

Discharge

Atm. NO3 Concentration

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CONTINUING RESEARCH

Currently waiting on isotope data from remaining storm samples. More data will help us better understand the relationship between storm size and sources of nitrate. Insight on relationships between the urban and rural watersheds.

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SPECIAL THANKS TO:

 Joel Bostic  Kevin Dodge  Rhonda Schwinabart  Robin Paulman  Pavithra Arachchige  Katie Kline  FRA’s  Graduate Students  Professors  Lab Staff