Tissue-wide interrogation and intervention of cellular - - PowerPoint PPT Presentation

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Tissue-wide interrogation and intervention of cellular - - PowerPoint PPT Presentation

May 03, 2023 43 likes •179 views

Tissue-wide interrogation and intervention of cellular electrophysiology with cell-type specificity Jia Liu, Ph.D. Assistant Professor of Bioengineering Harvard Bioelectronics Groups School of Engineering and Applied Sciences Harvard

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Tissue-wide interrogation and intervention of cellular electrophysiology with cell-type specificity

Jia Liu, Ph.D. Assistant Professor of Bioengineering Harvard Bioelectronics Groups School of Engineering and Applied Sciences Harvard University

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Single-neuron activities across entire organ

  • Brain functions are mostly associated with activities of neurons

from multiple, deep brain regions.

  • Different brain regions are highly innervated together through

neural projections. Many connections are based on single neuron. Connectivity Functionality

Elife, DOI: 10.7554/eLife.10566; Nature Meth. 10, 413 (2013); Nat. Rev. Neurosci. 8, 700 (2007)

What are challenges?

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What are challenges? Spatiotemporal dilemma

  • Phil. Trans. R. Soc. B 370, 20140171 (2017)

Time Scale Spatial Scale

Years (107 s) Milliseconds (10-3 s) Metres (100 m) Micrometres (10-6 m)

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Different types of cells interweave together

  • Nat. Rev. Neurosci. 18, 530 (2017)

What are challenges?

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Scalable technologies for tissue interface

Single cell Single spike

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Scalable technologies for tissue interface

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100 µm

Self-assemble inside needle and unfold after injection

Tissue-like mesh nanoelectronics

  • J. Liu, Y. Fang*, C. M. Lieber* et al. Nature Nano., 10, 629 (2015)
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Long-term single-cell electrophysiology in behaving animal

n = 4

  • Sci. Adv., 3, e1601966 (2017)
  • Proc. Natl. Acad. Sci. USA 114, 5894 (2017)
  • Nat. Methods 13, 875 (2016)
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Cyborg organoid model

Qiang Li, Kewang Nan, Paul Le Floch, Zuwan Lin, Hao Sheng, Jia Liu bioRxiv 697664; doi: https://doi.org/10.1101/697664

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Cyborg organoids

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Cyborg organoids

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Tissue-wide burst dynamics during tissue development

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Collaborators:

  • Prof. Karl Deisseroth (Stanford,

Bioengineering and Psychiatry and Behavioral Sciences)

  • Prof. Zhenan Bao (Stanford, Chemical

Engineering)

  • Prof. Charles M. Lieber (Harvard, Chemistry

and Chemical Biology)

  • Prof. Zhigang Suo (Harvard, Mechanical

Engineering)

  • Prof. Lihua Jin (UCLA, Mechanical

Engineering)

  • Prof. Seigiu Pasca (Stanford, Stem Cell

Center, Psychiatry and Behavioral Sciences)

  • Prof. Kang Shen (Stanford, Biology, HHMI)

Paul, Le Floch (ME)

  • Dr. Qiang Li (BioE)
  • Dr. Kewang Nan (MSE)

Hao Sheng (ME) Zuwan Lin (Chem)

  • Dr. Shiqi Guo (ECE)

Thomas Blum (BioE) Tianyu Shao (Physics) Vivi Song (Medicine) Qinyi Chen (CS) Xin Tang (EECS) Gaël Ancel (CS) Charlene de Guitaut (CS)

Acknowledgements

Jia Liu Group