The future of paper chromatography in the wine industry due to - - PowerPoint PPT Presentation

The future of paper chromatography in the wine industry due to alternative methods

Carie ien Coetzee

SAWLA Workshop 26 May 2017

Pap aper chr chromatography Acc ccuvin™ Vin Vinmetrica SC SC- 50 50 Enz Enzymatic ic Spe Spectrometric Ana nalysis is FT FTIR Win inescan HP HPLC

LOQ OQ Tim Time for

• r

ana analy lyses Haz azards Co Cost t per per sam ample le Ins nstrument cos

• st

Ease ase of

• f use

use Avail ilabilit ility

Simpler methods Complex methods

Kit contains

• Wide mouth jar
• Capillary pipettes
• Chromatography papers
• Chromatography solvent
• Tartaric acid standard
• Malic acid standard
• Lactic acid standard

Paper chromatography

Paper chromatography

• Small sample volume
• Limited training required
• Readily available
• Low cost
• Kit only
• 24 hours
• Hazardous chemicals
• Not quantitative
• Interpretation subjective

Malic acid Lactic acid

Paper chromatography

Kit contains

• Sampler
• Test strip
• Colour chart

Accuvin™

Accuvin™

• Small sample volume
• Limited training required
• NOT Readily available ?
• Low-medium cost
• Quantitative
• 4 minutes
• No Hazardous chemicals
• Interpretation subjective
• Kit only

• Vinmetrica SC-55 Manometer
• Malic Reagent set
• Biopressure Agent Reaction Vials
• 1 ‘check’ vial
• 10 ml serological pipette
• 15 ml conical tube
• Plastic bulb pipette
• 100 mL polypropylene beaker
• Plastic Scoop
• Distilled water
• Microwave oven

Vinmetrica SC-50

Vinmetrica SC-50

• Small sample volume
• Limited training required (more than previous)
• Not Readily available ?
• Low-medium cost
• Quantitative
• 30-60 minutes
• No Hazardous chemicals
• Need to acquire instrumentation + kit

• Spectrophotometer
• Cuvettes
• Micropipettes
• Enzyme test kit (includes buffer solution, NAD,

enzymes, standards)

Enzymatic Spectrometric Analysis

Enzymatic Spectrometric Analysis

• Small sample volume
• Laboratory training required
• Readily available
• Low-medium cost per sample
• Quantitative
• 30 minutes
• No Hazardous chemicals
• Need to acquire instrumentation + kit

Measure absorbance using a spectrophometer

Enzymatic Spectrometric Analysis

• Auto analyzers are convenient, but costly to purchase

✓ Eliminates analyst variation ✓ Less labour intensive ✓ Small volume needed ✓ Capacity for increased sample numbers ✓ Decrease in cost per sample

• Skilled personnel
• Periodic maintenance

• Foss Winescan / OenoFoss

FTIR Winescan

FTIR Winescan

• Winescan/OenoFoss is convenient, but costly to purchase

✓ Eliminates analyst variation ✓ Less labour intensive – very easy to use ✓ Small volume needed ✓ Capacity for increased sample numbers ✓ Decrease in cost per sample ✓ Automization available

• Skilled personnel
• Periodic maintenance
• Constant checks due to matrix effects
• Small sample volume
• Laboratory training required
• Low-medium cost per sample
• Quantitative
• 1 minute
• No chemicals
• Need to acquire instrumentation

• HPLC, column and assorted equipment
• Sample vials and caps
• Filtration membranes and syringes
• Acetonitrile, phosphoric acid, and distilled water
• Filtration unit for reagents

HPLC

HPLC

• Skilled personnel
• Periodic maintenance
• Small sample volume
• Laboratory training required
• High cost per sample
• Quantitative
• 40 minutes
• Potentially hazardous chemicals
• Need to acquire expensive

instrumentation

Pap aper chr chromatography Acc ccuvin™ Vin Vinmetrica SC SC- 50 50 Enz Enzymatic ic Spe Spectrometric Ana nalysis is FT FTIR Win inescan/OenoFoss HP HPLC

Qualitative Semi-Quantitative Quantitative Quantitative Quantitative Quantitative LOQ OQ n/a 0.03 g/L 0.1 g/L 0.05 g/L 0.001-0.05 g/L Tim Time for

• r

ana analy lyses 24 hours 4 min 30-60 min 30 min 1 min 40 min Haz Hazards chemicals noxious, flamable none irritant mildly corrosive none chemicals flamable, corrosive, irritant Co Cost t per per sam ample R7 R40-R50 R40-R50 R320 (after instrument) R650 (after instrument) Ins nstrument cos

• st

kit kit R2 000 R45 000 R1 200 000 R1 200 000 Ease ase of

• f use

use * * ** *** *** **** Avail ilabilit ility **** * * *** *** ***

Paper chromatography Accuvin™ Vinmetrica SC-50 Enzymatic Spectrometric Analysis FTIR Winescan HPLC

The future of paper chromatography in the wine industry due to alternative methods ✓ Easy to perform ✓ Readily available ✓ Low cost

• Useful for wineries: in-house checks
• Can then confirm with accredited laboratory the completion of MLF
• Not feasible in routine laboratories

Not Quantitative Does it need to be?

Questions?

carien@vinlab.com

SAWLA Workshop 26 May 2017