The future of paper chromatography in the wine industry due to - PowerPoint PPT Presentation

The future of paper chromatography in the wine industry due to alternative methods Carie ien Coetzee SAWLA Workshop 26 May 2017

Enzymatic Enz ic Pap aper Vin Vinmetrica SC SC- FTIR FT Acc ccuvin ™ Spe Spectrometric HPLC HP chr chromatography 50 50 Win inescan Ana nalysis is LOQ OQ Tim Time for or ana analy lyses Simpler methods Complex methods Haz azards Co Cost t per per sam ample le Ins nstrument cos ost Ease ase of of use use Avail ilabilit ility

Paper chromatography Kit contains • Wide mouth jar • Capillary pipettes • Chromatography papers • Chromatography solvent • Tartaric acid standard • Malic acid standard • Lactic acid standard

Paper chromatography • • Small sample volume 24 hours • • Limited training required Hazardous chemicals • • Readily available Not quantitative • • Low cost Interpretation subjective • Kit only

Paper chromatography Lactic acid Malic acid

Accuvin™ Kit contains • Sampler • Test strip • Colour chart

Accuvin™ • • Small sample volume Quantitative • • Limited training required 4 minutes • • NOT Readily available ? No Hazardous chemicals • • Low-medium cost Interpretation subjective • Kit only

Vinmetrica SC-50 • Vinmetrica SC-55 Manometer • Malic Reagent set • Biopressure Agent Reaction Vials • 1 ‘check’ vial • 10 ml serological pipette • 15 ml conical tube • Plastic bulb pipette • 100 mL polypropylene beaker • Plastic Scoop • Distilled water • Microwave oven

Vinmetrica SC-50 • Small sample volume • Quantitative • Limited training required (more than previous) • 30-60 minutes • Not Readily available ? • No Hazardous chemicals • Low-medium cost • Need to acquire instrumentation + kit

Enzymatic Spectrometric Analysis • Spectrophotometer • Cuvettes • Micropipettes • Enzyme test kit (includes buffer solution, NAD, enzymes, standards)

Enzymatic Spectrometric Analysis Measure absorbance using a spectrophometer • • Small sample volume Quantitative • • Laboratory training required 30 minutes • • Readily available No Hazardous chemicals • • Low-medium cost per sample Need to acquire instrumentation + kit

Enzymatic Spectrometric Analysis • Auto analyzers are convenient, but costly to purchase ✓ Eliminates analyst variation ✓ Less labour intensive ✓ Small volume needed ✓ Capacity for increased sample numbers ✓ Decrease in cost per sample • Skilled personnel • Periodic maintenance

FTIR Winescan • Foss Winescan / OenoFoss

FTIR Winescan • Winescan/OenoFoss is convenient, but costly to purchase ✓ Eliminates analyst variation ✓ Less labour intensive – very easy to use ✓ Small volume needed ✓ Capacity for increased sample numbers ✓ Decrease in cost per sample ✓ Automization available • Skilled personnel • • Quantitative Small sample volume • Periodic maintenance • • Laboratory training required 1 minute • Constant checks due to matrix effects • • Low-medium cost per sample No chemicals • Need to acquire instrumentation

HPLC • HPLC, column and assorted equipment • Sample vials and caps • Filtration membranes and syringes • Acetonitrile, phosphoric acid, and distilled water • Filtration unit for reagents

HPLC • Small sample volume • Laboratory training required • High cost per sample • Quantitative • 40 minutes • Potentially hazardous chemicals • Need to acquire expensive instrumentation • Skilled personnel • Periodic maintenance

Enzymatic Enz ic Pap aper Vin Vinmetrica SC SC- FTIR FT Acc ccuvin ™ Spe Spectrometric HPLC HP chr chromatography 50 50 Win inescan/OenoFoss Ana nalysis is Qualitative Semi-Quantitative Quantitative Quantitative Quantitative Quantitative LOQ OQ n/a 0.03 g/L 0.1 g/L 0.05 g/L 0.001-0.05 g/L Time for Tim or 24 hours 4 min 30-60 min 30 min 1 min 40 min ana analy lyses chemicals noxious, chemicals flamable, Haz Hazards none irritant mildly corrosive none flamable corrosive, irritant Co Cost t per per R320 (after R7 R40-R50 R40-R50 R650 (after instrument) sam ample instrument) Ins nstrument kit kit R2 000 R45 000 R1 200 000 R1 200 000 cos ost Ease ase of of use use * * ** *** *** **** Avail ilabilit ility **** * * *** *** ***

The future of paper chromatography in the wine industry due to alternative methods Enzymatic Paper Vinmetrica FTIR Accuvin™ HPLC Spectrometric chromatography SC-50 Winescan Analysis ✓ Easy to perform ✓ Readily available ✓ Low cost Not Quantitative Does it need to be? • Useful for wineries: in-house checks • Can then confirm with accredited laboratory the completion of MLF • Not feasible in routine laboratories

Questions? carien@vinlab.com SAWLA Workshop 26 May 2017