SLIDE 1 Synthetic Unity
Presented by Braxton Carter Daniel Griffin Wenjun Kong Dani Bauhan with much appreciation to Briana Harvey Michael Riddle Featuring Designs by Ayla Walter Advised by
- Dr. Richard Anthony
- Dr. Dave Goulet
Starring Victor the Vector as Educational Model with contributions from
SLIDE 2
- E. coli expresses Mating Factor α on the
cell surface with Ice Nucleation Protein.
SLIDE 3
Yeast construct links His3 expression to the Mating Pheromone α Response Pathway.
SLIDE 4
Yeast expresses Lactate Dehydrogenase to facilitate E. coli survival with lactate.
SLIDE 5
- E. coli construct links histidine expression to
the lactate inducible promoter.
SLIDE 6 Synthetic unity system creates a symbiotic relationship between Yeast and E. coli.
Yeast
SLIDE 7
Yeast expresses Blue Fluorescent Protein after binding of MFα with Fus1 promoter.
SLIDE 8
Victor the Vector provides better Synthetic Biology education for all students.
SLIDE 9
Victor the Vector is an electromechanical device that explores systems in ‘dry-lab.’
SLIDE 10
Victor the Vector interfaces with users through an interactive software platform.
SLIDE 11
After turning on Victor the Vector, the user selects a system to build.
SLIDE 12
The user builds the selected system on the Victor the Vector platform.
SLIDE 13
The software prompts the user about whether the system is built correctly.
Yes No
SLIDE 14 The program tells the user when they build the system correctly.
Video Time!
SLIDE 15
Victor the Vector is a pin-to-pin, voltage- gated circuit.
SLIDE 16
Poly-lactic acid (PLA) is a biodegradable plastic that we used for 3D printing.
SLIDE 17
Scrap PLA is hydrolyzed to create a lactic acid monomer solution.
SLIDE 18 Solution was characterized using a polarimeter to find the optical rotation.
pH Optical Rotation 2.5 .12 3.5
4.21
SLIDE 19
An LDH assay was used to determine the concentration of the final solution.
SLIDE 20
Team RHIT modeled synthetic unity to educate across diverse backgrounds and spread a uniting message.
SLIDE 21 Questions?
Special Thanks to:
- Dr. Richard Anthony
- Dr. Dave Goulet
- Dr. Alan Chiu
Shannon Tieken
