Short Status Presentation, August 2018 Jatropower AG - International - - PowerPoint PPT Presentation

Short Status Presentation, August 2018

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Jatropower AG - International spread

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Switzerland: Registerd office Jatropower India: 2 research farms on own land, 9 Seed Production Orchards. Operations: Research and Development, production of commercial cultivar seeds, store house, quality control laboratory

Starting year: 2008, key personnel in jatropha sector since >12 years Financing: private equity of the promoters Research collaboration: universities and public research institutes

Germany: Working office, near University of Hohenheim Jatropower Kenya Limited: Experimental station on own land. Operations: Agronomic research, Cultivar testing station

History and evolution

 Jatropower AG was founded in 2008 in Baar, Switzerland, Jatropower India founded immediately afterwards in Chennai, India  Objective was to collect jatropha seeds from farmers in co-operation with an Indian NGO, press oil and export to Europe for biodiesel production and sales  Jatropha plantations found not to be profitable for farmers – lack of good seeds and lack of knowledge

• n agronomy of the crop

 Start of the breeding programme in India by establishment of a genetic garden with genotypes from all jatropha “hot spots” of the world  Broad basing of genotype and product testing to Kenya (with own fully owned subsidiary)  Strengthening of, genotype base, agronomic data, knowledge packages and product portfolio through takeover of assets of Quinvita BV, Belgium in 2014 (generated through capital spend of over 8 million Euros)  The only Jatropha breeding company to have commercially sold improved jatropha seeds internationally and received repeat orders. The first company to market improved non-toxic jatropha seeds.

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Key business foci

 Agronomic observation under different conditions - since 2009, ongoing  Genetic analysis – ongoing, focus on identifying genetic distance, early hybrid confirmation, linking markers to key characters (company has SNP markers for pistillate character and non-toxicity marker, other marker-trait linkages being investigated)  Selection and breeding for first generation inbreds/synthetics – current portfolio has 7 cultivars and hybrids for sale, including the first elite non-toxic jatropha cultivar and 2 F1 Hybrid seeds  Seed production for sale – company operates 5 seed production orchards currently, including one where the first commercially marketed hybrid jatropha seeds is being produced  Hybridisation – JP hybridisation programme started in 2010 and several F1s with high hybrid vigour has been identified  Scalable production model for hybrids – JP currently has one scalable hybrid production system implemented  Evaluation of cultivar performance through co-operation with clients – agreement with a client to use their farm as a demo – farm and for continuous evaluation of products in pipeline  Client acquisition – seed purchase and/or technical consultancy agreements with 3 clients, discussions on with others Slide 4

JP Jatropha development recieved considerable momentum from the acquisition of Quinvita jatropha assets

 List of acquired assets:

 Site selection tool (google map based)  Complete agronomic knowledge base, manuals and presentations  Complete breeding knowhow and techniques  Complete „Agrobase“ database of containing all breeding and agronomic data so far  6 seed production orchards in India with selected QV genotypes  Selected elite QV genotypes and hybrids  Complete QV germplasm base including pistillate plants  Selected laboratory equipment (NMR for non-destructive dry matter and oil analysis, gas chromatograph for fatty acid analysis, bulk seed moisture meter, shelling machine for fruits)

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Non-toxic jatropha

 Jatropower has pioneered the development of high- yielding non-toxic jatropha seeds  Jatropower portfolio of seeds currently includes the first non-toxic jatropha cultivar to be marketed  First sales of non-toxic jatropha in 2015  Establishing new production facilities for non-toxic cultivars and derivative plants with higher yield

Q2, 2013

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Why non-toxic jatropha

 Jatropha kernel meal (not seed cake) has high quality as a protein rich animal feed ingredient  Conventional toxic jatropha meal has to be detoxified as they contain toxic phorbol ester (all other antinutrients present can be neutralised by heat treatment similar to soybean meal)  Detoxification is possible by solvent extraction (first shown at the University

• f Hohenheim during the „Daimler project“ in 2005-6)

 At least 4 detoxification patents exist, some since 2010 - but no commercially viable process yet because of the difficulty in integrating it with oil-extraction process and the high additional cost

 Basics of the process is simple and outlined in published material (e.g. Martınez- Herrera et al, Food Chemistry 96 (2006) 80–89)

 The non-toxic jatropha exist in nature

 Observations showed comparatively lesser yield compared to conventional jatropha  This however, changes when the selected cultivars and derivatives are used  No higher pest pressure compared to those observed on the toxic accessions

Q2, 2013

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Cultivar JPNT-1 (non-toxic, previously JP2)

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Further development of non-toxic jatropha cultivars

 Jatropower non-toxic cultivars do show a certain phenotypic and variation  The genetic distance measurements where repeated in 2018 with state-of-the-art techniques  A new selection of parents have been effected based

• n these and new NTxNT crosses have been initiated

 A further interesting source of NT variability comes from NT plants derived from apomixis  The present Jatropower pipeline has several promising cultivars/derivatives that will be offered shortly in the market

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JP research farms

 Several agronomic experiments are being conducted and results being evaluated

 Seedlings vs cuttings  Irrigation and fertilisation effects  Pruning trials  Pest control  Soil conditioning and micronutreints  Effects of chemical stimulants  Grafting trials Database considerably strenthened on most aspects by Quinvita data based on extensive trials

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On areas fully owned by the company: 10 acres plus 10 acres in India, 20 acres in Kenya

JPs hybridisation programme

 Jatropower has produced and is evaluating F1 hybrids and derivatives produced by crossing more than 20 elite selections  Markers developed help confirm hybrids early  Two of the hybrids have been selected for further evaluation and development, others under observation  Jatropower has evaluated Quinvita‘s hybridisation programme and selected some for performance evaluation and possibly further development  Generally the hybrids show high potential because

 Evident hybrid vigour in growth  Continuous flowering  Seed yield increase potential of over 50% over elite parents based on

• bservations so far

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Selection, and accelerated marker assisted varietal improvement

P1 = parent 1; H = hybrid and P2 = parent 2 5 sets of different primers (Lane 1-3 – primer 1, 4-6 – primer 2, 7-9 – primer 3, 10-12 – primer 4, 13-15 – primer 5

Q9, 2014

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M P1 H P2 P1 H P2 P1 H P2 P1 H P2 P1 H P2

Hybridity confirmation with markers

Markers for non-toxicity and pistillate nature identified Other marker-trait associations identified, further investigations on

The best JP hybrid so far (JPH1)

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Early and heavy bearing – One year old JPH1 in India

Big fruit bunches on all branches

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Scalable F1 hybrid production system using pistillate jatropha plants

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Pistillate J curcas with only female flowers Normal J curcas with male and female flowers The first scalable hybrid production farm established for the second Jatropower F1 hybrid, JPH2

In house laboratory facility

 Capabilities

 Seed morphology  Seed oil content  Non-destructive dry matter determination  Free fatty acid determination  Non-destructive seed oil determination (NMR to be installed this year)  Fatty acid composition of plant oil (GC to be installed this year)

 Being developed

 In vivo toxicity determination procedure for jatropha seeds/oil

 External collaboration

 Marker analysis  Fatty acid composition analysis  Phorbol ester analysis

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Other Jatropha species available at the farm for crossing trials

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 Jatropha gossypifolia  Jatropha mahafalensis  Jatropha glandulifera  Jatropha integerrima  Jatropha multifada  Jatropha podagrica  Jatropha pandurifolia  Jatropha tanjorensis  Jatropha platyphylla

JATROPOWER AG Haldenstrasse 5 CH-6340 Baar, Switzerland Contact:

• Dr. George Francis, CEO

Tel: +49 711 94542744 E-mail: info [at] jatropower.ch

Q9, 2014

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