Serum and urinary zinc level and erythrocyte superoxide dismutase - - PowerPoint PPT Presentation
Serum and urinary zinc level and erythrocyte superoxide dismutase activity in type 2 diabetic patients with different albuminuria categories Htun, Y.W 1 , Kyaw, M.P 1 , Thidar, A 1 , Maung, K.K 2 1 Department of Biochemistry, University of
Htun, Y.W1, Kyaw, M.P1, Thidar, A1, Maung, K.K2
1Department of Biochemistry, University of Medicine 2, Yangon. 2Department of Biochemistry, University of Medicine 1, Yangon.
mechanism involving in the development and progression of diabetic vascular complications. Aside from increased production of free radicals,
to impaired antioxidant defense mechanisms.
antioxidant trace element due to its diverse roles in antioxidant defense
maintain the functional conformation and stability of antioxidant enzyme copper-zinc superoxide dismutase.
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excretion in diabetic patients and might be related to albuminuria status.
detection of early stage of diabetic nephropathy and it also reflects higher risk of development of other diabetic vascular complications.
erythrocyte superoxide dismutase (SOD) activity among different albuminuria categories of type 2 diabetic patients as well as to demonstrate the correlation between the serum zinc level and erythrocyte superoxide dismutase activity in the study groups.
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albuminuria (n=86) groups. Albuminuria group was subdivided into microalbuminuria (n=53) and macroalbuminuria (n=33) groups.
immunoturbidimetric method and urine creatinine level measured by enzymatic method.
groups were analyzed by using unpaired ‘t’ test. A value of p ≤ 0.05 was considered as statistically significant.
parameters among the study groups.
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Normoalbuminuria (n=41) Albuminuria (n=86) p value (t test) Mean ± SD Mean ± SD Erythrocyte SOD (U/g Hb) 1955.95 ± 493.91 1480.33 ± 552.84 <0.001* Serum zinc (μg/dL) 108.39 ± 54.27 93.03 ± 55.89 0.146 Urinary zinc (mg/g creatinine) 0.72 ± 0.51 0.86 ± 0.66 0.262 Table 1. Comparison of erythrocyte superoxide dismutase activity, serum zinc and urinary zinc level between type 2 diabetic patients with normoalbuminuria and those with albuminuria
different albuminuria categories were insignificant, previous studies showed that serum zinc concentration was significantly lower in diabetic patients with increased albuminuria status.1
albuminuria group as compared to normoalbuminuria group. It supported the hypothesis of reduced antioxidant defense activity found with increased risk of vascular complications in diabetes as reflected by albuminuria status.2
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Group r p Significance Whole study population (n =127) 0.298 0.001 Not significant Whole study population Normoalbuminuria (n =41) 0.421 0.006 Significant Albuminuria (n = 86) 0.210 0.053 Not significant Albuminuria group Microalbuminuria (n = 53) 0.109 0.437 Not significant Macroalbuminuria (n = 33) 0.311 0.039 Significant Table 2. Correlation between serum zinc level and erythrocyte superoxide dismutase activity in different study groups
and erythrocyte SOD activity only in normoalbuminuria and macroalbuminuria groups.
and serum SOD activity in diabetic patients but some observed that there was no significant correlation between serum zinc level and erythrocyte SOD activity in diabetic patients. 3,4
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erythrocyte SOD activity was indeterminate since the correlation between the two factors was seen only in some study groups (normoalbuminuria and macroalbuminuria groups).
might be attributed by changes in serum zinc level but factors other than serum zinc level could also influence upon the activity of SOD enzyme.
vascular complications as indicated by many studies. But serum zinc level changes might also be the consequence of the increased urinary zinc excretion in diabetes.5
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Group r p Significance Whole study population (n =127)
0.400 Not significant Whole study population Normoalbuminuria (n =41)
0.774 Not significant Albuminuria (n = 86)
0.525 Not significant Albuminuria group Microalbuminuria (n = 53)
0.417 Not significant Macroalbuminuria (n = 33)
0.309 Not significant Table 3. Correlation between urinary zinc level and serum zinc level in different study groups Group r p Significance Normoalbuminuria (n =41)
0.774 Not significant Albuminuria (n = 86)
0.525 Not significant Table 4. Correlation between urine albumin creatinine ratio and urinary zinc level in different study groups
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normoalbuminuria group, it was not statistically significant in the study (Table 1). Moreover, correlation between urinary and serum zinc level as well as correlation between albuminuria level and urinary zinc level showed no significant correlation in different study groups (Table 3 and 4).
erythrocyte SOD activity changes in type 2 diabetic patients could be related to serum zinc status but factors other than serum zinc level should also be
explain the serum zinc level changes in type 2 diabetic patients.
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1. Luo, Y.Y., Zhao, J., Han, X.Y., Zhou, X.H., Wu, J. and Ji, L.N. (2015) Relationship between serum zinc level and microvascular complications in patients with type 2
2. Kornelia, Z., Kedziora-Kornatowska., Luciak, M., Blaszczyk, J. and Pawlak, W. (1998) Lipid peroxidation and activities of antioxidant enzymes in erythrocytes of patients with non-insulin dependent diabetes with or without diabetic nephropathy. Nephrology Dialysis Transplantation: 13; p.2829-2832. 3. Haddad, N.I.A., Nori, E. and Ali, S.H. (2016) The effect of type two diabetes mellitus
4. Salmonowicz, B., Krzystek-Korpacka, M. and Noczyńska, A. (2014) Trace elements, magnesium, and the efficacy of antioxidant systems in children with type 1 diabetes mellitus and in their siblings. Advances in Clinical and Experimental Medicine Journal: 23 (2); p.259–268. 5. Sagara, M., Kudo, M., Yashiro, H., Makino, I. and Takebe, K. (1982) Urinary excretion and serum levels of zinc in diabetics, and its relationship to renal damage. Journal of Japan Diabetes Society: 25(6); p.697-704.
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