RegionalTeamMeetingsand Bootcamp Fiveschoolsatthreecampuses - - PowerPoint PPT Presentation

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RegionalTeamMeetingsand Bootcamp Fiveschoolsatthreecampuses - - PowerPoint PPT Presentation

Dec 07, 2023 153 likes •455 views

RegionalTeamMeetingsand Bootcamp Fiveschoolsatthreecampuses Skype,email,Googlewave,andregionalmeetingsusedto communicatebetweencampuses.

slide-1
SLIDE 1
slide-2
SLIDE 2

Regional
Team
Meetings
and
 Bootcamp 


  • Five
schools
at
three
campuses

  • Skype,
email,
Google
wave,
and
regional
meetings
used
to


communicate
between
campuses.


  • Boot
camp
held
at
beginning
of
summer
to
bring
each
team


together.


slide-3
SLIDE 3

Quorum
Sensing
Amplifiers
 and
a
Co‐design
Approach
 for
Information
Processing


“Ditoxotector:
 Dual
Toxin
Detection”


slide-4
SLIDE 4

Motivation 


Applications
 Effect
 Environment
 Fish
‐
Most
heavy
metal
 combinations
 Health
 Human
liver
‐
Carbon
 Tetrachloride
and
Ethanol


slide-5
SLIDE 5

As

Toxins
alone… 


Hg

slide-6
SLIDE 6

As Hg

…but
with
two… 


slide-7
SLIDE 7

Goals 


  • Bacterial
biosensor
detects
simultaneous


presence
of
two
toxins


  • Modular
design
allows
for
extension/


improvement


slide-8
SLIDE 8

Input

Mercury Arsenic Copper

Project
Overview 


Amplification Logic/ Output

slide-9
SLIDE 9

Toxic
Metal
Detection 


slide-10
SLIDE 10

Arsenic
Switch


Promoter Operator ArsR

slide-11
SLIDE 11

Arsenic
Switch


Promoter Operator Operator ArsR GFP ArsR GFP ArsR As As

slide-12
SLIDE 12

Project
Overview 


Input

Mercury Arsenic Copper

Amplification Logic/ Output

slide-13
SLIDE 13

Quorum
Sensing
Design 


slide-14
SLIDE 14

Amplifier
Model 


Without the amplifier output is driven directly by the toxin switch. Dimer production is driven by the toxin switch as well as by positive feedback With the amplifier output is driven by the dimer.

slide-15
SLIDE 15

Amplifier
Model 


slide-16
SLIDE 16

Project
Overview 


Input

Mercury Arsenic Copper

Amplification Logic/ Output

slide-17
SLIDE 17

Logic
Structure 


Input
1
 (Hg)
 Input

2
 (Cu)
 Input
3
 (Ar)
 Detected
 ‐
 ‐
 ‐
 Detected
 ‐
 ‐
 ‐
 Detected
 Detected
 Detected
 ‐
 Detected
 ‐
 Detected
 ‐
 Detected
 Detected
 Detected
 Detected
 Detected
 Output
 Signal
A
 Output
 Signal
B
 Output
 Signal
C
 ‐
 ‐
 ‐
 ‐
 ‐
 ‐
 ‐
 ‐
 ‐
 Emitted
 ‐
 ‐
 ‐
 Emitted
 ‐
 ‐
 ‐
 Emitted
 Emitted
 Emitted
 Emitted


slide-18
SLIDE 18

Methods
for
AND
Logic


A
 B


Hybrid
Promoters
 Fluorescent
Complementation


slide-19
SLIDE 19
  • Constitutive
Promoters
J23110
&
J23106

  • Combination
of
2
operator
sites



Hybrid
Promoters


  • 35
  • 10

Tet Operator Lac Operator

  • 35
  • 10

Ara Operator Tet Operator

  • 35
  • 10

Ara Operator Lac Operator

slide-20
SLIDE 20

Our
System 


slide-21
SLIDE 21

Complementation


Magliery et. al 2005

slide-22
SLIDE 22

Top10 E. coli cotransformed with pUC19 and pSB2K3 plasmids.

Putting
it
all
together 


  • Co‐Transformation

  • Three
Antibiotic


Resistances
per
 Plasmid


slide-23
SLIDE 23

Used
for
creation
of
 customized
RBS
 sequences
for
the
PCR
 cassettes

 Used
to
evaluate
the
 effectiveness
of
the
current
 BioBrick
fluorescent
protein
 cassettes


Optimization


slide-24
SLIDE 24

AAAGAGGAGAAA RBS (only the Shine-Dalgarno)

11564.6 378.2

slide-25
SLIDE 25

Possible
Applications 


  • Environmental


‐
Replace
fluorescence
with
remediation


  • National
Security


‐
Bomb
–
Thiodigylcol
and
Phosphorous
 Trichloride
(Mustard
Gas)


slide-26
SLIDE 26

Conclusions 


  • Design
of
Quorum
Sensing
Amplifier

  • Construction
of
Hybrid
Promoters

  • Design
of
Complementation
System


slide-27
SLIDE 27

iGEM
Experience 


  • Regional
team
experiment
itself

  • Influence
on
project

  • Learning
to
communicate
and
work
together

  • Vital
experiences
to
the
future
of
synthetic


biology


slide-28
SLIDE 28

Acknowledgements
 


Our
Advisors 
 National
Science
Foundation 
 Mr.
Gene 
 Bluefield
State
College
 


slide-29
SLIDE 29