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Genes and standard components in plant engineering
Re-wiring plant genetic circuits - a paradigm shift from metabolic - - PowerPoint PPT Presentation
Re-wiring plant genetic circuits - a paradigm shift from metabolic - - PowerPoint PPT Presentation
Re-wiring plant genetic circuits - a paradigm shift from metabolic engineering to synthetic biology in plants Genes and standard components in plant engineering In the dark 3 weeks Callus ready Immature Immature embryo (2,4 D) for
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Donor plant Transgenic plant
Corn transformation using direct DNA transfer
Corn seeds = 14 dap Immature embryo (2,4 D) Immature embryo derived callus Callus ready for bombardment 3 weeks Callus tissue under phosphinothrici n selection Callus regenerating shoots 3-4 weeks darkness
To the light In the dark
3-4 weeks 3-4 cm tall Plant on rooting media
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Combinatorial nuclear transformation generates a metabolic library for the investigation of complex and multi-step biosynthetic pathways and the accumulation of specific combinations of metabolites
T Capell C Zhu Koreen Ramessar
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psy1+crtI
psy1+crtI+lycb+bch+crtW
Endosperm specific expression
S Naqvi C Zhu G Farre T Capell
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High provitamin A corn; 100-200 g of grain provide full RDI of β- carotene (as a sole source of vitamin A)
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5000 kg/ha 6600 kg/ha 2100 kg/ha 4100 kg/ha 8165.3 kg/ha
HCxMo17 HCxEZ6 A632 Mo17 EZ59 B73 HCxEZ59 EZ6
5300 kg/ha
HC Commercial hybrid HCxA632 HCxB73
11500 kg/ha 14900 kg/ha 9400 kg/ha 12400 kg/ha 10740 kg/ha 8100 kg/ha 7300 kg/ha
%H: 0.27 %H: 2.33 %H: 1.57 %H: 1.70 %H: 3.06
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R Alvez E Vilaprinyo
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Workflow for data-driven modeling of carotenoid biosynthesis in modified maize lines. For each line, experimental data were collected and used to interpolate dense time series of gene expression and metabolite concentrations. These time series were used to estimate the parameter values for an optimized mathematical model that best fits the experimental results for that line. A sensitivity analysis of the optimized model identified the genes whose overexpression was most likely to increase the accumulation of each metabolite. The expression of these genes was doubled and simulations were run to determine the effects
- f that doubling on metabolite concentration.
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Applications: Poultry feed
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Chicken feeding trials performed by J Diaz-Gomez, C Nogareda, JA Moreno & E Angulo, UdL Animal Production Dept
High carotenoid maize
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Time course of egg development in laying hens
BKT-keto diet HC-High Carotenoid diet
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J Estany
Higher and more mono-unsaturated intramuscular fat without increasing total fat content. Intramuscular to peripheral fat
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The transition from metabolic engineering to synthetic biology in plants
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Genome Editing in plants
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What is the outcome of editing?
HR
+ donor DNA + donor DNA + +
cassette insertion gene conversion
+ ssODN + + donor DNA +
small indels cassette insertion
NHEJ
large deletion inversion translocation
genome reorganizations
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Intervention strategies
- Supplements
- Fortification
- Biofortification
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Nutritional engineering
- Most populations are simultaneously deficient for multiple
nutrients
- Solving one nutritional deficiency merely shifts the problem
to another
- The only way to avoid this is to simultaneously engineer