PROSPECTS FOR ALGAE ARI PATRINOS SYNTHETIC GENOMICS INC. Role of - - PowerPoint PPT Presentation
PROSPECTS FOR ALGAE ARI PATRINOS SYNTHETIC GENOMICS INC. Role of Metabolic Engineering in Industrial Biotechnology Targeted manipulation of metabolic pathways Products from metabolic engineering Ethanol 1,3-Propanediol
ARI PATRINOS SYNTHETIC GENOMICS INC.
Products from metabolic engineering
– Ethanol – 1,3-Propanediol – Polyhydroxyalkanoates – Organic acids – Vitamins – Amino acids – Nutritional oils
Improved bioprocess economics
– Product yield (g/g) – Product concentration (g/L) – Productivity (g/L/h)
Targeted manipulation of metabolic pathways
Microalgae have exceptionally high oil productivity and growth rates compared to terrestrial plants
– Cost vs plant biodiesel feedstock – Low photosynthetic efficiency – Limited genetic tools
– Improve photosynthetic efficiency using novel photoreceptors – Increase oil content to reduce cost – Tailor fatty acid composition for biodiesel
CO2 in the process of photosynthesis
more, 1 km2 of algae could capture 30,000 MT of CO2 per year CO2 Sunlight Seawater
Algae
Lipids Hydrocarbons Alcohols Hydrogen
CO2 +
Methyl Esters
pathway
– Amplification of any one gene does not increase pathway flux
variation in kinetic properties
behavior of a complex pathway
– Rational selection of best pathway genes difficult
– Species selection – Nutrient supply – Growth module design
– Classical mutagenesis – Metabolic engineering
– Utilize new genome information – Introduce entire pathways – Test multiple gene configurations – Eliminate side reactions
Cutting-edge technology that enables the design and construction of synthetic chromosomes that optimize production
Anneal overlapping synthetic oligonucleotides DNA fragments Overlapping fragments assembled by homologous recombination Chromosome Transplantation
Synthetic Chromosome Synthetic Chromosome
Anneal overlapping synthetic oligonucleotides DNA fragments Overlapping fragments assembled by homologous recombination Chromosome Transplantation
Synthetic Chromosome Synthetic Chromosome
From reading to writing the genome code …and synthesizing cells that make useful products
Pharmaceuticals Substitutes for Petrochemicals Bio-Energy
T A G C
Sunlight Plant Biomass CO2 DNA Synthesizer Completely assembled Synthetic Chromosome