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perspectives for using in tetraploid potato breeding JAROSAW PLICH - - PowerPoint PPT Presentation

Oct 09, 2023 118 likes •356 views

Gene Sen2 perspectives for using in tetraploid potato breeding JAROSAW PLICH I H A R - P I B M O C H W R E S E A R C H C E N T E R , P O L A N D Synchytrium endobioticum (Schilberszky) Percival Yield losses: 50-100% Soil

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Gene Sen2 – perspectives for using in tetraploid potato breeding

JAROSŁAW PLICH

I H A R - P I B

M Ł O C H Ó W R E S E A R C H C E N T E R , P O L A N D

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Synchytrium endobioticum (Schilberszky) Percival

Yield losses: 50-100% Soil contamination (spores viable > 40 year) Quarantine pathogen Resistant potato cultivars

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Resistance against

  • S. endobioticum

Pathotype 1(D1)

  • Major locus Sen1 on chromosome XI

(Hehl et al. 1999)

  • Major locus Sen1-IV chromosome IV

(Brugmans et al. 2006)

~40 virulent pathotypes

  • QTLs on chromosomes I, IX

(Ballvora et al. 2011)

  • QTLs on chromosomes I, II, VI, VIII, VIII, X, XI

(Groth et al. 2013)

  • Locus on chr. XI + QTLs on all potato chromosomes

(Obidiegwu et al. 2015)

  • Locus on chromosome XI – pathotypes 6, 18

(Bartkiewicz et al. 2018)

  • Sen3 - chromosome XI – pathotypes 2, 6, (18)

(Prodhomme et al. 2019)

  • Sen2 – chromosome XI – broad spectrum resistance

(Plich et al.2018)

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Potato clone

DG 97-264

resistant against eight pathotypes:

1 (D1) 2 (G1) 6 (O1) 8 (F1) 18 (T1) 2 (Ch1) 3 (M1) 39 (P1)

Gene Sen2 – broad-spectrum resistance

Most important in Europe Identified in Poland

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Gene Sen2 in diploid potato progeny

DG 97-264

DG 97-1805

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Gene Sen2 in diploid potato progeny

Sen2

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Gene Sen2 – genomic localization

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Gene Sen2 – introducing into tetraploid potato genepool

DG 97-264

Resistant to 8 pathotypes 1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

DG 01-591

Resistant to 8 pathotypes, 2n gamets 1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

4x – 2x

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Gene Sen2 – introducing into tetraploid potato genepool

DG 01-591

Resistant to 8 pathotypes, 2n gamets 1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

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Gene Sen2 – introducing into tetraploid potato genepool

4x – 2x 4x – 4x

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R - 33 R - 33 R - 33 R - 33 R - 33 R - 33 R - 33 R - 33

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DG 97-264 DG 97-1805 Ancestor 1 Ancestor 2 DG 01-591 M 62 819 11-10-48 Deodara Tomensa

SEN 14-01 i 14-02

R R R S S S S

5450_3 (TasI) 2502_1 (HinfI) 32_502

Mapping population

SEN 12-01 DG 97-264 DG 97-1805

Gene Sen2 – DNA markers

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DG 97-264 DG 97-1805 Ancestor 1 Ancestor 2 DG 01-591 M 62 819 11-10-48 Deodara Tomensa

SEN 14-01 i 14-02

R R R S S S S

5450_3 (TasI) 2502_1 (HinfI) 32_502

Gene Sen2 – DNA markers

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DG 97-264 DG 97-1805 Ancestor 1 Ancestor 2 DG 01-591 M 62 819 11-10-48 Deodara Tomensa

SEN 14-01 i 14-02

R R R S S S S

5450_3 (TasI) 2502_1 (HinfI) 32_502

Gene Sen2 – DNA markers

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DG 97-264 DG 97-1805 Ancestor 1 Ancestor 2 DG 01-591 M 62 819 11-10-48 Deodara Tomensa

SEN 14-01 i 14-02

R R R S S S S

5450_3 (TasI) 2502_1 (HinfI) 32_502

Gene Sen2 – DNA markers

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Is gene Sen2 present in tetraploid potato cultivars?

Cultivar/clone Resistance to (some) virulent pathotypes Markers for Sen2 Y1del AAT1 Kc81032 Markers for Sen33 DG 97-264

1

1 1 DG 01-591

1

1 1 R-I-3 1 1 1 Adam 1 1 1 1 Antares 1 Apollo 1 Bałtyk 1 Bzura 1 1 1 1 Cekin 1 1 1 1 Gandawa 1 1 1 1 Gawin 1 1 1 1 Giewont 1 Ibis

1

1 1 1

1 –Obidiegwu et al. 2015; 2 – Bartkiewicz et al. 2018; 3- results from paper Prodhomme et al. 2019

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Cultivar/clone Resistance to (some) virulent pathotypes Markers for Sen2 Y1del AAT Kc8103 Markers for Sen3 Igor

1

1 1 Ikar 1 1 1 1 Karolin 1 1 1 1 Kuba 1 1 1 1 Megusta 1 1 1 Miriam 1 1 Nicola 1 Ora 1 1 1 1 Otolia 1 ? Palladia 1 1 1 Rudawa

1

1 1 1 Saphir

1

Zagłoba 1 1 1 1

Is gene Sen2 present in tetraploid potato cultivars?

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Cultivar/clone Resistance to (some) virulent pathotypes Markers for Sen2 Y1del AAT Kc8103 Markers for Sen3 Ari Bryza Calrose Carpatin Cayuga Dalila Sarpo Mira Sebago Seneca Wauseon

Is gene Sen2 present in tetraploid potato cultivars?

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A new project: Sen2Breed

Using of Sen2 gene in breeding of new potato cultivars resistant to virulent pathotypes of Synchytrium endobioticum, causal agent of potato wart disease

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The aim of Sen2Breed

▪ To show benefits of introduction of the gene Sen2 into new potato cultivars ▪ To present advantages of using DNA markers linked with Sen2 in breeding programs ▪ To develop advanced potato breeding lines possessing the gene Sen2 (single or in combination with other resistance sources e.g. Sen1, Sen3)

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Take-home message

▪ Gene Sen2 was identified and mapped to potato chromosome XI ▪ Sen2 provides high level of resistance for broad spectrum of S. endobioticum pathotypes in diploid as well as in tetraploid potatoes ▪ Potato cultivars with Sen2 will available… soon (relatively soon ☺)

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Acknowledgments

Jarosław Plich Ewa Zimnoch- Guzowska Jarosław Przetakiewicz Beata Tatarowska Bogdan Flis Technical Staff