perspectives for using in tetraploid potato breeding JAROSAW PLICH - PowerPoint PPT Presentation

Gene Sen2 – perspectives for using in tetraploid potato breeding JAROSŁAW PLICH I H A R - P I B M Ł O C H Ó W R E S E A R C H C E N T E R , P O L A N D

Synchytrium endobioticum (Schilberszky) Percival Yield losses: 50-100% Soil contamination (spores viable > 40 year) Quarantine pathogen Resistant potato cultivars

Resistance against S. endobioticum • Major locus Sen1 on chromosome XI (Hehl et al. 1999) Pathotype 1(D1) • Major locus Sen1 -IV chromosome IV (Brugmans et al. 2006) • QTLs on chromosomes I, IX (Ballvora et al. 2011) • QTLs on chromosomes I, II, VI, VIII, VIII, X, XI (Groth et al. 2013) • Locus on chr. XI + QTLs on all potato chromosomes ~40 virulent (Obidiegwu et al. 2015) pathotypes • Locus on chromosome XI – pathotypes 6, 18 (Bartkiewicz et al. 2018) • Sen3 - chromosome XI – pathotypes 2, 6, (18) (Prodhomme et al. 2019) • Sen2 – chromosome XI – broad spectrum resistance (Plich et al.2018)

Gene Sen2 – broad-spectrum resistance Potato clone DG 97-264 resistant against eight pathotypes: 1 (D1) 6 (O1) 8 (F1) 18 (T1) 2 (Ch1) 3 (M1) 39 (P1) 2 (G1) Most important in Europe Identified in Poland

Gene Sen2 in diploid potato progeny DG 97-264 DG 97-1805

Gene Sen2 in diploid potato progeny Sen2

Gene Sen2 – genomic localization

Gene Sen2 – introducing into tetraploid potato genepool DG 97-264 Resistant to 8 pathotypes 1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1) 4x – 2x DG 01-591 Resistant to 8 pathotypes, 2n gamets 1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

Gene Sen2 – introducing into tetraploid potato genepool DG 01-591 Resistant to 8 pathotypes, 2n gamets 1(D1) 2(G1) 6(O1) 8(F1) 18(T1) 2(Ch1) 3(M1) 39(P1)

Gene Sen2 – introducing into tetraploid potato genepool 4x – 2x 4x – 4x

R - 33 R - 33 R - 33 R - 33 R - 33 R - 33 R - 33 R - 33

Gene Sen2 – DNA markers DG 97-1805 SEN 14-01 i 14-02 Ancestor 2 DG 01-591 Ancestor 1 DG 97-264 M 62 819 11-10-48 Tomensa Deodara R S S R R S S 5450_3 ( Tas I) DG 97-264 2502_1 Mapping ( Hinf I) population SEN 12-01 DG 97-1805 32_502

Gene Sen2 – DNA markers DG 97-1805 SEN 14-01 i 14-02 Ancestor 2 DG 01-591 Ancestor 1 DG 97-264 M 62 819 11-10-48 Tomensa Deodara R S S R R S S 5450_3 ( Tas I) 2502_1 ( Hinf I) 32_502

Gene Sen2 – DNA markers DG 97-1805 SEN 14-01 i 14-02 Ancestor 2 DG 01-591 Ancestor 1 DG 97-264 M 62 819 11-10-48 Tomensa Deodara R S S R R S S 5450_3 ( Tas I) 2502_1 ( Hinf I) 32_502

Gene Sen2 – DNA markers DG 97-1805 SEN 14-01 i 14-02 Ancestor 2 DG 01-591 Ancestor 1 DG 97-264 M 62 819 11-10-48 Tomensa Deodara R S S R R S S 5450_3 ( Tas I) 2502_1 ( Hinf I) 32_502

Is gene Sen2 present in tetraploid potato cultivars? Y1del AAT 1 Kc8103 2 Cultivar/clone Resistance to (some) Markers for Sen2 Markers for Sen3 3 virulent pathotypes DG 97-264 1 1 0 1 DG 01-591 1 1 0 1 R-I-3 1 1 1 0 Adam 1 0 1 1 1 Antares 1 0 0 0 0 Apollo 1 0 0 0 0 Bałtyk 1 0 0 0 Bzura 1 0 1 1 1 Cekin 1 0 1 1 1 Gandawa 1 0 1 1 1 Gawin 1 0 1 1 1 Giewont 1 0 0 0 0 Ibis 0 1 1 1 1 1 – Obidiegwu et al. 2015; 2 – Bartkiewicz et al. 2018; 3 - results from paper Prodhomme et al. 2019

Is gene Sen2 present in tetraploid potato cultivars? Cultivar/clone Resistance to (some) Markers for Sen2 Y1del AAT Kc8103 Markers for Sen3 virulent pathotypes Igor 0 1 1 1 Ikar 0 1 1 1 1 Karolin 0 1 1 1 1 Kuba 0 1 1 1 1 Megusta 0 1 1 1 Miriam 0 1 0 0 1 Nicola 0 0 0 1 Ora 0 1 1 1 1 Otolia 0 0 ? 0 1 Palladia 0 1 1 1 Rudawa 0 1 1 1 1 Saphir 0 0 0 0 1 Zagłoba 0 1 1 1 1

Is gene Sen2 present in tetraploid potato cultivars? Cultivar/clone Resistance to (some) Markers for Sen2 Y1del AAT Kc8103 Markers for Sen3 virulent pathotypes Ari 0 0 0 0 Bryza 0 0 0 0 Calrose 0 0 0 0 Carpatin 0 0 0 0 Cayuga 0 0 0 0 Dalila 0 0 0 0 Sarpo Mira 0 0 0 0 Sebago 0 0 0 0 Seneca 0 0 0 0 Wauseon 0 0 0 0

A new project: Sen2Breed Using of Sen2 gene in breeding of new potato cultivars resistant to virulent pathotypes of Synchytrium endobioticum , causal agent of potato wart disease

The aim of Sen2Breed ▪ To show benefits of introduction of the gene Sen2 into new potato cultivars ▪ To present advantages of using DNA markers linked with Sen2 in breeding programs ▪ To develop advanced potato breeding lines possessing the gene Sen2 (single or in combination with other resistance sources e.g. Sen1 , Sen3 )

Take-home message ▪ Gene Sen2 was identified and mapped to potato chromosome XI ▪ Sen2 provides high level of resistance for broad spectrum of S. endobioticum pathotypes in diploid as well as in tetraploid potatoes ▪ Potato cultivars with Sen2 will available… soon (relatively soon ☺ )

Acknowledgments Jarosław Plich Ewa Technical Zimnoch- Staff Guzowska Jarosław Bogdan Flis Przetakiewicz Beata Tatarowska