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- Dr. Reinard
- Prof. Dr. Peterhänsel
Prof. Dr. Peterh nsel Dr. Reinard 2 Hannove ve r Bo Boston 3 - - PowerPoint PPT Presentation
Prof. Dr. Peterh nsel Dr. Reinard 2 Hannove ve r Bo Boston 3 - - PowerPoint PPT Presentation
Prof. Dr. Peterh nsel Dr. Reinard 2 Hannove ve r Bo Boston 3 4 - global issue - harmful to animals and human - dose response effect - accumulation 5 - cleaning nature by using nature itself - equip plants with a protein which binds
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Hannove ver Bo Boston
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- global issue
- harmful to animals and human
- dose response effect
- accumulation
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- cleaning nature by using nature itself
- equip plants with a protein which binds
- four different heavy metals
- significant reduction
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Top 4 Metal Binding Protein signal sequence (Exp4) Cellulose Binding Domain
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Plant t Vector tor
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- a new plant vector for iGEM
(plasmid map originally taken from www.snapgene.com/resources)
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promoter exchange: enTCUP2 promoter into 2x35s promoter removal of restriction sites: XhoI and BglII
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GFP
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evaluate:
- the signal sequence (Exp4)
- cellulose-binding domain (CBD)
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EXP CBD Term R1 R2
Exponential Megaprimer PCR
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pORE_E3_2 _E3_2x35 x35S_G S_GFP FP
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expression of protein
signal sequence transient transformation
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Protein ein
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Nicotiana tabacum
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Nicotiana tabacum
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- E. coli
- time
- tag
- amount of protein
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- E. coli
Anti-6xHistidine Tag Antibody
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seeds were harvested
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selective medium
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heterologous expression in E. coli binding of Zn, Cd and Cu regeneration of potentially transgenic plants
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Teabag
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Hannove ver Bo Boston
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Exponential Megaprimer PCR
1st step 2nd step
Ulrich et al. PLoS One. 2012;7(12)
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