prof dr peterh nsel dr reinard
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Prof. Dr. Peterh nsel Dr. Reinard 2 Hannove ve r Bo Boston 3 - PowerPoint PPT Presentation

Prof. Dr. Peterh nsel Dr. Reinard 2 Hannove ve r Bo Boston 3 4 - global issue - harmful to animals and human - dose response effect - accumulation 5 - cleaning nature by using nature itself - equip plants with a protein which binds


  1. Prof. Dr. Peterh ä nsel Dr. Reinard 2

  2. Hannove ve r Bo Boston 3

  3. 4

  4. - global issue - harmful to animals and human - dose response effect - accumulation 5

  5. - cleaning nature by using nature itself - equip plants with a protein which binds - four different heavy metals - significant reduction 6

  6. signal sequence (Exp4) Cellulose Binding Domain T op 4 M etal B inding P rotein 7

  7. Plant t Vector tor 8

  8. - a new plant vector for iGEM 9 (plasmid map originally taken from www.snapgene.com/resources)

  9. promoter exchange: enTCUP2 promoter into 2x35s promoter removal of restriction sites: XhoI and BglII 10

  10. GFP 11

  11. evaluate: - the signal sequence (Exp4) - cellulose-binding domain (CBD) 12

  12. Exponential Megaprimer PCR R1 R2 EXP CBD Term 13

  13. pORE_E3_2 _E3_2x35 x35S_G S_GFP FP 14

  14. transient transformation expression of p rotein signal sequence 15

  15. Protein ein 16

  16. 17

  17. Nicotiana tabacum 18

  18. - time - tag Nicotiana tabacum - amount of protein E. coli 19

  19. E. coli Anti-6xHistidine Tag Antibody 20

  20. 21

  21. 22

  22. seeds were harvested 22

  23. selective medium 23

  24. heterologous expression in E. coli binding of Zn, Cd and Cu regeneration of potentially transgenic plants 24

  25. Teabag 0 25

  26. Hannove ve r Bo Boston 26

  27. Exponential Megaprimer PCR 1 st step 2 nd step Ulrich et al. PLoS One. 2012;7(12)

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