products Surrogate reporter Human-AAVS1 / Mouse-Rosa26 Kit. KO / - - PowerPoint PPT Presentation

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products Surrogate reporter Human-AAVS1 / Mouse-Rosa26 Kit. KO / - - PowerPoint PPT Presentation

May 30, 2023 274 likes •533 views

aRGEN (Synthesis, Validation) CRISPR/CAS9 dRGEN (Synthesis, Validation) CAS9 protein & mRNA(WT, Nickase, Dead, Cy3) products Surrogate reporter Human-AAVS1 / Mouse-Rosa26 Kit. KO / KI cell line Genome KO / KI / cKO

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  • aRGEN (Synthesis, Validation)
  • dRGEN (Synthesis, Validation)
  • CAS9 protein & mRNA(WT, Nickase, Dead, Cy3)
  • Surrogate reporter
  • Human-AAVS1 / Mouse-Rosa26 Kit.

CRISPR/CAS9 products

  • KO / KI cell line
  • KO / KI / cKO Mouse
  • KO Rat
  • Donor synthesis (ssDNA, dsDNA)

Genome Editing service

  • Genotyping
  • Only Miseq. running

In-del Analysis service

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Gene Function study Mutagenesis study Ex vivo, In vivo, Cell therapy, Gene therapy Animal model, Cell line model Stem cell engineering, plant & Animal genome editing (Molecular Breeding) Micro algae engineering, fungi, insect

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Species

  • Human
  • Mouse
  • Rat
  • Zebrafish

End product

  • Living
  • rganism
  • Cell line
  • Primary cell

Gene

  • One
  • Multi

Type

  • KO
  • Double KO
  • KI
  • Double KI
  • cKO

(reversion) (base editing)

gRNA+Cas9

  • RNA + Protein

(RNP)

  • DNA + DNA

(one, two vector)

  • DNA + Protein
  • RNA + mRNA

Transfect

  • Reagent
  • Electroporation
  • Lentivirus, AAV

Detect

  • Transfection

Marker

  • Surrogate

reporter

Confirm

  • In-del

analysis (NGS)

  • Genotyping

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Cat No# TGEN_aRS1 – Product : aRGEN(sgRNA. 50ug) +pRGEN-Cas9-CMV/T7 (5ug) – Service Period : 2~3 week – Warranty period : 4 month

* Activity 별도 확인 필요

Target gene gRNA Design by ToolGen gRNA form : RNA (액체 or 동결 건조 형태로 제공) CAS9-CMV plasmid 제공(Protein 형태는 별도 구매)

* off-target information

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Cat No# TGEN_aRS1 – Product : aRGEN(sgRNA. 50ug) +pRGEN-Cas9-CMV/T7 (5ug) – Service Period : 2~3 week – Warranty period : 4 month

* Activity 별도 확인 필요

Target gene gRNA Design by ToolGen gRNA form : RNA (액체 or 동결 건조 형태로 제공) CAS9-CMV plasmid 제공(Protein 형태는 별도 구매)

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Cat No# TGEN_aRV1 gDNA(1~2ug) be given for other than Human / Mouse – Product : aRGEN(sgRNA. 50ug) + pRGEN-Cas9-CMV/T7 (5ug) + Purified Cas9 protein (50ug) → In vitro assay – Service Period : 4~5 week – Warranty period : 4 month Cat No# TGEN_aRV2 Cell be given for other than Human / Mouse – Product : aRGEN(sgRNA. 50ug) + pRGEN-Cas9-CMV/T7 (5ug) + Purified Cas9 protein (50ug) → Cell line assay – Service Period : 4~5 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : RNA (액체 or 동결 건조 형태로 제공) In vitro assay로 Activity 확인된 gRNA 제공 (모든 종에서 가능) CAS9-CMV plasmid & CAS9 protein 모두 제공 Target gene gRNA Design by ToolGen gRNA form : RNA (액체 or 동결 건조 형태로 제공) In cell(in vivo) assay로 Activity 확인된 gRNA 제공 (Only Human & Mouse) CAS9-CMV plasmid & CAS9 protein 모두 제공

HEK293, NIH3T3 * Activity 확인 완료

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Cat No# TGEN_aRSP1 – Product : Postive control_EGFP sgRNA (20ug) – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_aRSP2 – Product : Postive control_HPRT sgRNA (20ug) – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_aRSP3 – Product : Postive control_CCR5 sgRNA (20ug) – Service Period : 2 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : RNA (액체 or 동결 건조 형태로 제공) CAS9-CMV plasmid 제공(Protein 형태는 별도 구매) * gRNA(aRGEN) 구매 시에 activity Positive control로 사용할 수 있는 제품

* Activity 확인 완료

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Cat No# TGEN_dRS1 – Product : 1 dRGEN ( sgRNA plasmid, 1~2ug) + pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_dRS2 – Product : 1 dRGEN ( sgRNA plasmid, 50ug) + pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) CAS9-CMV plasmid 제공 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) 고농도로 제공 (Midi prep. 으로 제공) CAS9-CMV plasmid 제공

Cat No# TGEN_dRPS1 – Product

  • 3 dRGEN ( sgRNA plasmid, 1~2ug)
  • pRGEN-Cas9-CMV/T7(5ug)

– Service Period : 2~3 week – Warranty period : 2 month Cat No# TGEN_dRPS2 – Product

  • 3 dRGEN ( sgRNA plasmid, 50ug)
  • pRGEN-Cas9-CMV/T7(5ug)

– Service Period : 2~3 week – Warranty period : 2 month

* Activity 별도 확인 필요

Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) target에 대한 gRNA 3개 제공 CAS9-CMV plasmid 제공 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) target에 대한 gRNA 3개 제공 고농도로 제공 (Midi prep. 으로 제공) CAS9-CMV plasmid 제공

Promoter Promoter

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* Activity 별도 확인 필요

Cat No# TGEN_dRS3 – Product : CRISPR-GFP plasmid(dRGEN, 1~2ug)+ pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_dRS4 – Product : CRISPR-GFP plasmid(dRGEN, 50ug)+ pRGEN-Cas9-CMV/T7(5ug) – Service Period : 2~3 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) GFP 형광으로 Transfection efficiency check (세포내에서 계속적으로 발현하지는 않음) 저/고농도로 제공 CAS9-CMV plasmid 제공

Cat No# TGEN_OP1 – Product : able to buy individual pRGEN-Cas9-selection marker – pRGEN-Cas9-CMV/T7 – pRGEN-Cas9-CMV/T7 (wild type or Puro-RFP or Hygro-EGFP) – pRGEN-Cas9-Ef1a (wild type or Puro-RFP or Hygro-EGFP) – pRGEN-Cas9-CMV/T7 (nickase(D10A) type or Puro-RFP or Hygro-EGFP) – pRGEN-Cas9-Ef1a (nickase(D10A) type or Puro-RFP or Hygro-EGFP) – Service Period : 2 week – Warranty period : 4 month

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Cat No# TGEN_dRV1 Possible to provide only in the Human/Mouse – Product

  • Validated 1 dRGEN ( sgRNA plasmid, 1~2ug)
  • pRGEN-Cas9-CMV/T7(5ug)
  • data/setup condition (T7E1 assay)

→ possible to provide additional dRGEN 1ea – Service Period : 4~5 week – Warranty period : 4 month Cat No# TGEN_dRV2 Possible to provide only in the Human/Mouse – Product

  • Validated 1 dRGEN ( sgRNA plasmid, 1~2ug)
  • pRGEN-Cas9-CMV/T7(5ug)
  • data/setup condition (FACS assay)

→ possible to provide additional dRGEN & Reporter 1ea – Service Period : 4~5 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) Transfection 진행 후 T7e1 assay로 Activity 확인된 gRNA 제공 (only Human & Mouse) CAS9-CMV plasmid 제공 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) Human cell에 Transfection 진행 후 FACS assay로 Activity 확인된 gRNA 제공 (모든 종 확인 가능) Surrogate reporter 제공 (MACS/Hygro/RG2S) CAS9-CMV plasmid 제공

* Activity 확인 완료

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Cat No# TGEN_CP1 – Product : Cas9 WT protein, Injection ready, lyophilized,50ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CP2 – Product : Cas9 WT protein, Injection ready, lyophilized, 100ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CP3 – Product : Cas9 WT protein, Injection ready, lyophilized, 250ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CP4 – Product : Cas9 WT protein, Injection ready, lyophilized, 500ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_Cy3P1/2 – Cy3 Cas9 protein, solution, 25μg/50ug

form : Protein (액체 or 동결 건조 형태로 제공) 다양한 농도 및 양으로 제공 form : Protein (액체 형태로 제공) 다양한 농도 및 양으로 제공 가능 Cy3 형광 tagging으로 check 가능

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Cat No# TGEN_CNP1 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 50ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CNP2 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 100ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CNP3 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 250ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CNP4 – Product : nickase(D10A) Cas9 protein, Injection ready, lyophilized, 500ug – Service Period : 2 week – Warranty period : 4 month

form : Protein (액체 or 동결 건조 형태로 제공) 다양한 농도 및 양으로 제공 가능

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Cat No# TGEN_CDP1 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 50ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CDP2 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 100ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CDP3 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 250ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CDP4 – Product : dead(D10A/840A) Cas9 protein, Injection ready, lyophilized, 500ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_Cy3DP2/4 – Cy3 Cas9 dead protein, solution, 50ug/250ug

form : Protein (액체 or 동결 건조 형태로 제공) 다양한 농도 및 양으로 제공 가능 form : Protein (액체 형태로 제공) 다양한 농도 및 양으로 제공 가능 Cy3 형광 taq으로 check 가능

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Cat No# TGEN_CR1 – Product : Cas9 mRNA, lyophilized, 10ug – Service Period : 2 week – Warranty period : 4 month Cat No# TGEN_CNR1 – Product : nickase(D10A) Cas9 mRNA, lyophilized, 10ug – Service Period : 2 week – Warranty period : 4 month

CAS9 WT form : mRNA (액체 or 동결 건조 형태로 제공) CAS9 nickase(D10A) form : mRNA (액체 or 동결 건조 형태로 제공)

Cat No# TGEN_T7E1

  • T7E1(200rxn)_1ea
  • 10X T7E1 buffer_1ea
  • Control product

Form : enzyme (액체 형태로 제공) gRNA activity 확인 시에 이용

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Cat No# TGEN_dRRR1 – Product : ToolGen dRGEN RG2S (RFP-GFP-GFP, Kan-R) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_dRRM1 – Product : ToolGen dRGEN MACS RS (RFP-GFP-H2KK, Kan-R) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_dRRH1 – Product : ToolGen dRGEN Hygro RS (RFP-GFP, Hygro-R, Kan-R) – Service Period : 2~3 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) 형광으로 발현 확인 가능 2XGFP형태 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) 형광으로 발현 확인 가능 H2KK 있어 MACS sorting selection 가능 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) 형광으로 발현 확인 가능 Hygro-R selection 가능

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Cat No# TGEN_dRRR1 – Product : ToolGen dRGEN RG2S (RFP-GFP-GFP, Kan-R) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_dRRM1 – Product : ToolGen dRGEN MACS RS (RFP-GFP-H2KK, Kan-R) – Service Period : 2~3 week – Warranty period : 4 month Cat No# TGEN_dRRH1 – Product : ToolGen dRGEN Hygro RS (RFP-GFP, Hygro-R, Kan-R) – Service Period : 2~3 week – Warranty period : 4 month

Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) 형광으로 발현 확인 가능 2XGFP형태 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) H2KK 있어 MACS sorting selection 가능 Target gene gRNA Design by ToolGen gRNA form : DNA (액체 형태로 제공) Hygro-R selection 가능

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Cat No# TGEN_AAVS1 – AAVS1 RGEN Knock-In Kit

  • pRGEN_Hs_AAVS1-U6_SG (2ug)
  • pRGEN_Cas9(CMV) (5ug)
  • AAVS1 T7E1 primer set(100pM, 35ul)
  • AAVS1_donor vector(2ug)

Cat No# TGEN_Rosa26 – Rosa26 RGEN Knock-In Kit

  • pRGEN_Mm_Rosa261-U6_SG(2ug)
  • pRGEN_Cas9(CMV)(5ug)
  • Rosa26 T7E1 primer(100pM, 35ul)
  • Rosa26 _donor vector(2ug)

Targeted Transgene Insertion into the Human-AAVS1 Locus gRNA form : DNA (액체 형태로 제공) Activity 확인된 gRNA(AAVS1 target) 제공 KI (stable cell line) 시 이용할 수 있는 Donor 제공 PCR primer 제공 CAS9-CMV plasmid 제공 Targeted Transgene Insertion into the Mouse-Rosa26 Locus gRNA form : DNA (액체 형태로 제공), RNA (동결 건조 형태로 제공) Activity 확인된 gRNA 제공 Mouse 종에서 KI 시 이용할 수 있는 Donor 제공 PCR primer 제공 CAS9-CMV plasmid 제공

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Cat No# TGEN_CKO1 – Product : Commonly-used cell line (HeLa, HCT116 recommended, HepG2, Hep3B, HEK293, et al) – Service Period : 3~6 month – Warranty period : 1 Year Cat No# TGEN_CKI1 – Product : point mutation, gene insertion Specific insertion of reporter gene into target locus (included price of Donor DNA design and synthesis) – Service Period : 3~6 month – Warranty period : 1 Year Cat No# TGEN_aRSP3 – Product : Toolgen Cell line option service (If allele is more than 3) Cat No# TGEN_CPP2 – Product : Park2 hetero knockout (+/-) pig fibroblast cell line

Target gene gRNA Design by ToolGen form : Cell (Stock 형태로 제공) Sequencing으로 out of mutation 확인 Target gene gRNA Design by ToolGen form : Cell (Stock 형태로 제공) Sequencing으로 KI 부위 확인 KI : point mutation, 다양한 selection marker 삽입…

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Cat No# TGEN_MKO1 – Product

  • F0 mutant mouse with >10% mutation rate
  • C57BL/6N strain is used for KO (Option : Sperm freezing & embryo freezing )

– Service Period : 4 Month – Warranty period : 6 Month Cat No# TGEN_MKI1 – Product

  • F0 mutant mouse with >10% mutation rate
  • C57BL/6N strain is used for KI (Option : Sperm freezing & embryo freezing )

(If Donor cloning is necessary, price will be calculated based on size) – 1kb is 200,000 KRW – Service Period : 8~12 Month – Warranty period : 6 Month Cat No# TGEN_MCKO1 – Product

  • F0 mutant mouse with >10% mutation rate
  • C57BL/6N strain is used for KO (Option : Sperm freezing & embryo freezing )

– Service Period : 8~12 Month – Warranty period : 6 Month

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Cat No# TGEN_SSD1

  • Single Strand Donor(100uM. 40ul)

Cat No# TGEN_DSD1

  • Double Strand Donor(5ug) (less than 2kb)

Cat No# TGEN_DSD2

  • Double Strand Donor(5ug)

(between 2kb and 3kb, additional charge when more than 3kb)

고객이 원하는 내용으로 design 진행 Form : ssDNA (액체 형태로 제공) Length : 100mer 이하 고객이 원하는 내용으로 design 진행 Form : DNA (액체 형태로 제공) Length : 2KB 이하 고객이 원하는 내용으로 design 진행 Form : DNA (액체 형태로 제공) Length : 2KB 이상

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TGEN_NGS1 – Targeted Resequencing (using MiSeq) – 1 sample < X < 90 sample TGEN_NGS2 – Requesting the Only MiSeq running.

Indel analysis 를 위해 고객이 library를 제작하여 제공하면, 기기를 돌림. 고객에게 대용량 결과 파일을 제공

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In-del analysis 를 위해 고객이 gDNA형태의 sample을 제공하면, Miseq.으로 확인할 수 있게 library를 제작하여 기기를 돌림. 얻어진 data를 분석하여, 고객에게 결과 파일을 제공

8bp del (X2469) CCCCTAGACTCTTCACTGCCTGAGGACATTC--------ACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTG 10bp del (X2434) CCCCTAGACTCTTCACTGCCTGAGGAC----------TTACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTG 5bp del (X2277) CCCCTAGACTCTTCACTGCCTGAGGACATTCCGG-----ACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTG

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E-mail : Salesplanning@toolgen.com Homepage : Http://www.Toolgen.com Phone : 02-873-8168 Address : #1210, 12F., Byucksan Digital Valley 6-cha, 219 Gasan Digital 1-ro, Geumcheon-gu, Seoul, South Korea. 153-704