PHYSICS OF BIOLOGICAL SYSTEMS ph549 LECTURE 14 Life in crowded - - PowerPoint PPT Presentation

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Apr 04, 2024 318 likes •595 views

PHYSICS OF BIOLOGICAL SYSTEMS ph549 LECTURE 14 Life in crowded environments Ideal vs. Real cells Rob Philips, Physical Biology of the Cell Protein Census in E. Coli Census in E. Coli Census in E. Coli The crowded Cytoskeleton Rob

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PHYSICS OF BIOLOGICAL SYSTEMS – ph549

LECTURE 14 – Life in crowded environments

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Ideal vs. Real cells

Rob Philips, Physical Biology of the Cell

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Protein Census in E. Coli

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Census in E. Coli

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Census in E. Coli

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The crowded Cytoskeleton

Rob Philips, Physical Biology of the Cell

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The crowded Cytoskeleton

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Diffusion and FRAP

Rob Philips, Physical Biology of the Cell

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Diffusion and FRAP

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Diffusion in membranes

Rob Philips, Physical Biology of the Cell

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Diffusion in membranes

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Diffusion in crowded environments

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Order through disorder

Rob Philips, Physical Biology of the Cell

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Depletion Interaction

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Depletion Interaction

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Depletion Interaction

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Discs near a surface (2D)

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Two large spheres

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Two large spheres

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Helices through crowding

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Why don’t all large structures in the cell aggregate?

Electrostatic interactions
Dynamic structures (cytoskeleton/vesicles)
Active processes (energy spent in preventing aggregation)
Constrained motion (due to cytoskeleton/…)

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References and further reading

Physical Biology of the Cell – Rob Phillips et. al. Chapter 14
The depletion attraction: an underappreciated force driving cellular
rganization, Marenduzzo et. al. JCB, 175, 681 (2006)