PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 - - PDF document
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Courtney Phillips Wednesday, March 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 1 PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14,
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 1
Courtney Phillips Wednesday, March 14, 2012
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 2
Insecticide, Fungicide & Rodenticide Act
Federal Food, Drugs & Cosmetics Act
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 3
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 4
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 5 SPE Quechers Dilution Automated variants Liquid Chromatography* MS/MS High Resolution Mass Analyzer
Quechers Dilution Automated variants Liquid Chromatography* MS/MS High Resolution Mass Analyzer
Solid Phase Extraction Separates analytes of interest from a complex matrix Phases similar to LC columns Requires vacuum manifold
~ 5 – 10 min/sample
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 6 SPE Quechers Dilution Automated variants Liquid Chromatography* MS/MS High Resolution Mass Analyzer
Developed specifically for pesticides analysis in foods Created as an alternative to the Luke method Requires centrifuge
~ 5 – 10 min/sample
SPE Quechers Dilution Automated variants Liquid Chromatography* MS/MS High Resolution Mass Analyzer
Mass analyzers require lower flow rates UHPLCs have the ability to run at these flow rates However, traditional UHPLC columns may not be ideal – Consider Superficially Porous Particle (SPP) columns
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 7 SPE Quechers Dilution Automated variants Liquid Chromatography* MS/MS High Resolution Mass Analyzer
SPE Quechers Dilution Automated variants Liquid Chromatography* MS/MS High Resolution Mass Analyzer
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 8
Acephate Diazinon Spinosad A Dimethoate Mevinphos Prometryne/dichloran Oxamyl Malathion/metalochlor Anilazine Methomyl Acetamiprid Mevinphos isomers
Pesticides by LC-TOF
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 9
Pesticides by LC-TOF
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 10
Spiked Pesticides in Celery
Extraction by Quechers RP-LC Pos ion mode Dual probe ESI- TOF with lock mass calibration
RP-HPLC Brownlee C18 SPP 10cm Gradient from 30%
Ambient temp, 0.3mL/min Flow m/z 100-400 1.5 spectra/sec Drying Gas Temp 350oC Dual ESI Source with Lock Mass Drying Gas Flow 12 L/min Capillary exit voltage = 90 V
Carbendazim in Orange Juice
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 11
RP-HPLC Brownlee C18 SPP 10cm Gradient from 30%
Ambient temp, 0.3mL/min Flow m/z 100-400 1.5 spectra/sec Drying Gas Temp 350oC Dual ESI Source with Lock Mass Drying Gas Flow 12 L/min Capillary exit voltage = 90 V
Carbendazim in Orange Juice
RP-HPLC Brownlee C18 SPP 10cm Gradient from 30%
Ambient temp, 0.3mL/min Flow m/z 100-400 1.5 spectra/sec Drying Gas Temp 350oC Dual ESI Source with Lock Mass Drying Gas Flow 12 L/min Capillary exit voltage = 90 V
Carbendazim in Orange Juice
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 12
Carbendazim in Orange Juice All data, all the time…
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 13
All data, all the time… All data, all the time…
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 14
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PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 15
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 16
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 17
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 18
PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 19
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PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 20
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PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 For more information, contact courtney.phillips@perkinelmer.com 22
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