PARTNER K Cell irradiation experiments Inform models of: Tumour - - PDF document

PARTNER

K Ki kb & R j J Karen Kirkby & Raj Jena

• Cell irradiation experiments
• Inform models of:
• Tumour response to radiation
• Different types of radiation
• Different fractionation patterns
• Efecfts of chemotherapeutic agents

Efecfts of chemotherapeutic agents

• Clinical trials
• Tissue models

Cell irradiation experiments

• Targeted single cell irradiation
• Precisely counted single ions (H-Ar)

S b ll l t ti (10 )

Cell irradiation experiments

• Sub-cellular targeting accuracy (10nm)
• High throughput (100,000 cellls/ hr) requires scanned focused

beam

• Ion beam analysis of “living” cells in culture
• Sub-cellular spatial resolution (100nm)
• Elemental mapping using scanned beam

Elemental mapping using scanned beam

• Horizontal sample orientation is highly desirable
• Liquid culture media

q

• Happy cells!
• Add chemotherapeutic agents

And of course we need a housing for this equipment … q p

widefield parfocal image planes

OPTICAL DETECTION END-STATION

environment t l

excitation widefield detection

• bjectives

control

laser scanning

f wide throat stage

scanning widefield t

focus g

trans- illumination

beam focusing focusing and scanning

Experiments Experiments

• Standard cell lines
• protons and ions
• protons and ions
• cell cycle phase
• Patient specific cell lines (TIC) 32 – GBM(12)

Patient specific cell lines (TIC) 32 – GBM(12)

• Protons and ions
• Radio-sensitivity – differentiation cascade

Radio sensitivity differentiation cascade

• Effects of chemotherapeutic agents /hypoxia
• Effects of chemotherapeutic agents (linked into clinical trial)
• Effects of hypoxia for protons and different ions

Training Objectives Training Objectives

• Standard cell lines
• Cell handling
• Cell handling
• Use of irradiation facilities
• Mathematical modelling

Mathematical modelling

• Patient specific cell lines (TIC) 32 – GBM(12)
• Cell handling

Cell handling

• modelling
• Chemotherapeutic agents /hypoxia
• Chemo handling / dose escalation
• Hypoxic cell clamp

• Migrating glioma cell

Migrating glioma cell

• Patient -specific glioma cells in spheroid form

G12 TIC ll G12 TIC cells Colin Watts

Conclusions Conclusions

• Proven track record of bringing clinical research and basic science

together g

• “Toolbox” of modelling techniques with innovative applications, which

complements existing radio-biological modelling

• Full spectrum of research training opportunities for patient specific

radiation biology experiments