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Classical Sterile Insect Technique via Irradiation … several success stories in agriculture.
- Screw Worm Fly
- Mediteranean Fruit Fly
- Pink Boll Worm
Non Transgenic Replacement and Suppression of Vector Mosquitoes - - PDF document
Non Transgenic Replacement and Suppression of Vector Mosquitoes - - PDF document
3/21/2016 Non Transgenic Replacement and Suppression of Vector Mosquitoes Stephen Dobson 13 March, 2016 Summit on the Aedes aegypti Crisis in the Americas: Joining Together to Address a Grand Challenge Macei, Brazil 1 3/21/2016
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Classical Sterile Insect Technique via Irradiation … but hasn’t worked well with mosquitoes
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X X X X
Bidirectional Incompatibility Bidirectional Incompatibility
Aedes aegypti Scott O’Neill
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Wolbachia‐based Strategies
- Population Replacement
- Release of Wolbachia infected females
- Goal: NOT intended as a pesticide; no reduction of the
- Ae. aegypti population
- Goal: infect local populations of mosquitoes with Wolbachia,
which is intended to interfere with virus transmission
- Wolbachia Suppression
- Release of Wolbachia infected males
- Goal: reduce mosquito densities with sterile male releases
(similar to insecticides)
- Aedes albopictus
- Aedes aegypti
Wolbachia‐based Strategies
- Population Replacement
- Release of Wolbachia infected females
- Goal: NOT intended as a pesticide; no reduction of the
- Ae. aegypti population
- Goal: infect local populations of mosquitoes with Wolbachia,
which is intended to interfere with virus transmission
- Wolbachia Suppression
- Release of Wolbachia infected males
- Goal: reduce mosquito densities with sterile male releases
(similar to insecticides)
- Aedes albopictus
- Aedes aegypti
Self‐sustaining
Interference: Fitness Costs Zika? other viruses? Potential virus selection Not self‐sustaining (unless achieve elimination) Successful examples of SIT Wolbachia does not negatively effect male fitness
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http://www.epa.gov/oppbppd1/biopesticides Regulatory Consideration – Wolbachia Microbial Pesticide
Lexington, KY
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Los Angeles, CA Fire Island, NY Lexington, KY
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Auto-Dissemination by Autocidal Males (ADAM)
Hormone Treatment
- Effects conspecifics in the same breeding sites
- Interspecific and Intraspecific effect
Clovis, CA Lexington, KY
Aedes albopictus Aedes aegypti
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Lexington, Kentucky
Robert ‘Bob’ Rose, PhD Kentucky Cabinet for Economic Development