Non Transgenic Replacement and Suppression of Vector Mosquitoes - PDF document

3/21/2016 Non ‐ Transgenic Replacement and Suppression of Vector Mosquitoes Stephen Dobson 13 March, 2016 Summit on the Aedes aegypti Crisis in the Americas: Joining Together to Address a Grand Challenge Maceió, Brazil 1

3/21/2016 Classical Sterile Insect Technique via Irradiation … several success stories in agriculture. • Screw Worm Fly • Mediteranean Fruit Fly • Pink Boll Worm 2

3/21/2016 Classical Sterile Insect Technique via Irradiation … but hasn’t worked well with mosquitoes 3

3/21/2016 Bidirectional Incompatibility Bidirectional Incompatibility X X X X Scott O’Neill Aedes aegypti 4

3/21/2016 Wolbachia ‐ based Strategies • Population Replacement • Release of Wolbachia infected females • Goal: NOT intended as a pesticide ; no reduction of the Ae. aegypti population • Goal: infect local populations of mosquitoes with Wolbachia , which is intended to interfere with virus transmission • Wolbachia Suppression • Release of Wolbachia infected males • Goal: reduce mosquito densities with sterile male releases (similar to insecticides) • Aedes albopictus • Aedes aegypti Wolbachia ‐ based Strategies • Population Replacement • Release of Wolbachia infected females • Goal: NOT intended as a pesticide ; no reduction of the Ae. aegypti population • Goal: infect local populations of mosquitoes with Wolbachia , which is intended to interfere with virus transmission Self ‐ sustaining Zika? other viruses? Potential virus selection Interference: Fitness Costs • Wolbachia Suppression • Release of Wolbachia infected males • Goal: reduce mosquito densities with sterile male releases (similar to insecticides) Successful examples of SIT Not self ‐ sustaining • Aedes albopictus (unless achieve elimination) • Aedes aegypti Wolbachia does not negatively effect male fitness 5

3/21/2016 Regulatory Consideration – Wolbachia Microbial Pesticide http://www.epa.gov/oppbppd1/biopesticides Lexington, KY 6

3/21/2016 Fire Island, NY Los Angeles, Lexington, KY CA 7

3/21/2016 Auto-Dissemination by Autocidal Males (ADAM) Hormone Treatment Effects conspecifics in the same breeding sites • • Interspecific and Intraspecific effect Aedes aegypti Clovis, CA Lexington, KY Aedes albopictus 8

3/21/2016 Robert ‘Bob’ Rose, PhD Lexington, Kentucky Kentucky Cabinet for Economic Development 9