New Developments at Axiogenesis 09.06.2016 Hamamatsu User Meeting - - PowerPoint PPT Presentation

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New Developments at Axiogenesis 09.06.2016 Hamamatsu User Meeting - - PowerPoint PPT Presentation

Mar 24, 2023 444 likes •606 views

Company Cooperation Products &Services Marketing Financials New Developments at Axiogenesis 09.06.2016 Hamamatsu User Meeting Dr. Ralf Kettenhofen Barcelona, Spain Presented for: Company Characterization Product/Format Service

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SLIDE 1
  • Presented for:

Financials Cooperation Products &Services Marketing Company

New Developments at Axiogenesis

09.06.2016 Hamamatsu User Meeting Barcelona, Spain

  • Dr. Ralf Kettenhofen
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  • Presented for:

Characterization Product/Format Service Company

Content

Optogentics Contraction Force - The Cor.4CE System Matured Cor.4U Cardiomyocytes

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  • Presented for:

Financials Cooperation Products &Services Marketing Company

Optogenetics Encoded Calcium Sensors

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  • Presented for:

Characterization Product/Format Service Company

Baculovirus Transduced Gene Delivery and the Fluorescent Sensor

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  • Presented for:

Characterization Product/Format Service Company

Baculovirus Transduced Cor.4U Cardiomyocytes - Fluorescent Microscopy

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3d after transduction 50x

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  • Presented for:

Characterization Product/Format Service Company

First Recordings in the FDSS 7000EX

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3d after transduction

Isoproterenol

200 400 600 800 1000 1200 1400 1600 Vehicle Control 1.0 10 30 100 amplitude [FU] concentra3on [nM] Baseline 5 min 10 min 20 min 10 20 30 40 50 60 70 80 90 100 Vehicle Control 1.0 10 30 100 beat rate [bpm] concentra.on [nM] Baseline 5 min 10 min 20 min

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  • Presented for:

Characterization Product/Format Service Company

Encoded Calcium Sensor Modified Cor.4U Cardiomyocytes

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Quality control of cells before start of assay. Long-term recording and study of chronic toxicity in Cor.4U cardiomyocytes become possible in the FDSS
 => Requirements are: Measurement in the FDSS under sterile conditions (with lid) Cell culture condition in the FDSS (CO2 + humidity) or attachment to a cell hotel Automated repeated recording (e.g. over night) Higher throughput (parallelization): bar code reader and automated recording

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SLIDE 8
  • Formats

Characterisation Applications Services Company

Cor.4CE

Cor.4U and FibroCor.4U Co-Culture

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SLIDE 9
  • Formats

Characterisation Applications Services Company

The CellDrum System

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p: Pressure h: Deflection r: Radius s: Thickness

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SLIDE 10
  • Formats

Characterisation Applications Services Company

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a: Amplitude b1, b2: Slopes c1, c2: Durations d1, d2: Integrals

The CellDrum System

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SLIDE 11
  • Formats

Characterisation Applications Services Company

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The New 24 Well CellDrum System

Top view Bottom view

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SLIDE 12
  • Presented for:

Financials Cooperation Products &Services Marketing Company

Matured Cor.4U Cardiomyocytes

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  • Presented for:

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6 weeks culture on a non-stiff cell culture surface

Matured Cor.4U Cardiomyocytes

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  • Presented for:

Characterization Product/Format Service Company

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cardiac alpha actinic nuclei

Matured Cor.4U Cardiomyocytes

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Thank you!

www.axiogenesis.com info@axiogenesis.com

  • rder@axiogenesis.com

Axiogenesis AG

Nattermannallee 1 50829 Cologne Germany tel: +49 221 99 88 18 - 0 fax: +49 221 9988 18 -10