Nanoparticles; Optical and Biomedical Applications Jaebeom Lee - - PowerPoint PPT Presentation

▶

Mar 12, 2024 136 likes •260 views

Nanoparticles; Optical and Biomedical Applications Jaebeom Lee Department of Nanomedical Engineering Pusan National University Re-construction Nanomaterials assemblies Expected better or novel optical properties of combined

SLIDE 1

Nanoparticles; Optical and Biomedical Applications

Jaebeom Lee

Department of Nanomedical Engineering

Pusan National University

SLIDE 2

Re-construction

Expected better or novel
ptical properties of combined

nano-hybrids

Customized optical and

electromagnetic properties of nanomaterials

Possibly advanced nano

devices application for environmental and bio-sensing, solar cell, photovoltaics, imaging, non-linearity, photonics, optoelectronics, etc.

Combination to have Function Individual

Nanomaterials assemblies

SLIDE 3

Stabilizers

Water-based Synthesis
Control the sizes of NP
Easy to bind other materials

Nanoparticles, Nanorods

CdTe, CdSe, CdS, HgTe
Au, Ag

SLIDE 4

Z. Tang, N. A. Kotov, M. Giersig. Science 2002, 297, 273.

Quantum yield 29% Quantum yield 16% Quantum yield 2.3% Quantum yield 6%

Strong Luminescence Aqueous Dispersions Nanoparticle Nanowires

SLIDE 5

SA Biotin Au NP

EDC, Sulfo-NHS

Biotin

NP or NW

CdTe NW-Biotin

Centrifuge

6000 rpm, 10 min, 3 times

EDC, Sulfo-NHS

Au or CdTe NP-Streptavidin

Centrifuge

6000 rpm, 10 min, 3 times HS(CH2)2CH3 : HS(CH2)2COOH (3:1)

24 hrs stirring

Immobilization

EDC : 1-ethyl-3-(3-dimethlamino propyl) carbodiimide hydrochloride Sulfo-NHS : N-hydroxy-sulfosuccinimide

SLIDE 6

Excitation (420 nm)

Au NP 689 nm

SA-B

550 600 650 700 750 800

1 2 3 4 5 NW-biotin only 2 min 30 min 65 min Wavelength (nm)

Wavelength (nm)

550 600 650 700 750 800

2 4 6 8 1.0 1.2 1.4 1.6

NW-biotin 40 min

PL Intensity

A: NW-Biotin with Streptavidin B: NW-Biotin with Au-Streptavidin

Microscopic images

J Lee, Nano Lett.; 2004; 4(12); 2323-2330

Luminescence Enhancement via Nanowires and Au Nanoparticles Assembles

5-fold PL enhancement B A

001 100

Before conjugation After conjugation

SLIDE 7

Temperature oscillations result in emission modulation

sec sec

Stretch! Depending on Temperature

CdT e PEG (∆r) Au

SLIDE 8

Sensing is based on wavelength shift

f the emission

intensity Not on the intensity change

No need for internal standards Robust operation Tunable wavelengths

Litmus paper technology for biosensors

J. Lee, Nature Materials, Apr. 2007

SLIDE 9

The local surface of NW is not flat, inducing different band gaps. One probable reason:

SLIDE 10

Interdisciplinary study is necessary to develop

novel nanoscale biomedical sensing/imaging devices

The superstructures using polymer/ bio-

affinity and nanomaterials have strong potential for future sensing/imaging devices

SLIDE 11

Funding Agencies:

New faculty allowance, Pusan National University
2 yr Unassigned Project, Pusan National University
New Faculty Research Project Award (2 yrs from Aug. 2007), KRF
Bone patch development, IGB fraunhoefer JRC
Fluorescent NP detector in PCR, IGB fraunhoefer JRC
3rd IT research center, MKE
Brain Korea 21 (support stipends of graduates)

Industrial Collaboration

Sungjin J&T, Busan
HAp Inc., Busan
SeedBiochip Inc. Chungju Ochang BT park

Academic Collaboration

Nicholas A. Kotov U of Michigan Alexander O. Govorov Ohio Uni. Zhiyong Tang Peking Univ.

Prof. K.N. Koh

Nanomedical Dept. , PNU

Prof. S.H. Jin
Chem. & Edu. Dept.
Prof. S.C. Ryu

Nanomaterials Dept.

Prof. S.H. Kim

Nanosystem & Proc. Dept.

Prof. C.M. Kim

Medical School

Graduates

Dr Hongxia Chen, Post-doc Fangfang Sun Master degree

Undergraduate Assistants

Hyung Goo Kang, Sangjun Park, SungHoon Kim, Kyung-Geun Lim