Nanoparticle based biosensors for diagnostics Arben Merkoi 1,2 , - - PowerPoint PPT Presentation
Nanoparticle based biosensors for diagnostics Arben Merkoi 1,2 , Marisa Maltez 2 1 ICREA 2 Institut Catal de Nanotecnologia. CIN2 (ICN-CSIC),campus UAB arben.merkoci.icn@uab.cat Jornada Nano i Micro Tecnologia pel diagnstic Medic Parc de
1 ICREA 2 Institut Català de Nanotecnologia. CIN2 (ICN-CSIC),campus UAB
Jornada Nano i Micro Tecnologia pel diagnòstic Medic Parc de Recerca UAB - Barcelona, 28th October 2010
http://www.fluigence.com/ http://www.medicaldisposablespr.com/
Easy to use Low Cost Lower time Miniaturization
Biocompatibility Rapid and simple chemical synthesis Excellent electroactivity Efficient coating by biomolecules Electrochemical advantages Low sample volumes (30μL) Single use Miniaturization/portability
Potentiostat Palm PC
Target Receptor
Au Ag Au 2 H + H 2
Current Au Au Current Cd Cd Current
De la Escosura et al. Chem. Com. 2010
Nanotechnology 2009
L.O.D. 0.198μg/mL DNA target
L.O.D. 1.82μg/mL DNA target
L.O.D. 65 pg/mL HIgG
Ambrosi et al. Anal. Chem. 2007
AuNP/ αHIgG-HRP HIgG ΜΒ/α-HIgG
Ag+ Ag+ Ag+ Au Au Ag+ Au Au Ag+ Ag+ Ag+ Au Au Ag+ Au Au Ag+ Au Au Ag+ Au Au Au Au Au Au Ag+ Au Au Au Au Au Au
AuNP/αHIgG HIgG ΜΒ/α-HIgG
De la Escosura et al.
L.O.D. 23 fg/mL HIgG
Maltez da Costa et al.
L.O.D. 1.45 ng/mL HIgG
De la Escosura et al. Electrochem. Com. 2010
Using the catalytic effect of AuNPs
Collaboration w ith Dr. A. González ( UV)
De la Escosura et al, Biosen. Bioelectron. 2010
L.O.D: 3 mUI HBV Ab / mL
Superparamagnetic Micro beads HBsAg anti-Hum IgG/AuNP
Human serum
Project Valtec num ber 0 8 -1 -0 0 0 7
Specific Protein/ Superparamagnetic Micro beads
Breast cancer marker
Specific Antibody /AuNP
0.5 1 1.5 2 2.5 3 50 100 150 200 250 CA15-3 (U/mL) O.D. (450 nm)
Ambrosi et al, Anal. Chem. 2010
TMB
LOD: 4000 cells/ 700 μL sample
H+ 2H+ H2 H+ 2H+ H2
a a’ b c b’ c’ d d’
H+ 2H+ H2 H+ 2H+ H2
a a’ b c b’ c’ d d’
HMY: Tum oral hum an B cell line w ith expressed HLA-DR m olecules PC3 : Tum oral hum an prostate cell line
A B A B
De la Escosura et al. Anal. Chem. 2009
Using the catalytic effect of AuNPs
Collaboration w ith Dr. A. González ( UV)
M.T.Castañeda (UNAM, Mexico)
H.Takeda (USP, Brasil)
Ü. Kirgöz (MU, Turkey)