N-Process Improvement of N mineral nutrition Grassland Agro - PowerPoint PPT Presentation

N-Process Improvement of N mineral nutrition Grassland Agro Conference R&D TAI Roullier Group JM Garcia-Mina (JGM v1 2014)

• N fertilization – Main Topics – N root uptake – N fertilizer metabolic efficiency within the plant – N losses: Efficiency and environmental problems – Soil N mobilization

N fertilization Problems – N-Process solutions – N root uptake – NP1 PMA swc action – N fertilizer metabolic efficiency within the plant – NP1 PMA swc syn. actions – N losses: Efficiency and environmental problems – NP2 NS TA Action NAU nutrition – Soil N immobilization – NP3 SEA 1 Action

N-Process action on soil-plant system  N transformation  N root uptake PMA SWC Root  N Fert - units – NAU effect  Gradual N release Rhizosphere - Matrix NS TA  Soil Microbial Activity  Soil Enzymatic activities (mainly In granule Soil Proteases and ureases ) N leaching SEA 1 N water - soil contamination SOIL  A NH3 volatilization vailable fractions of nutrients NUTRIENTS in soil, mainly inorganic nitrogen -

• NP1 (PMA SWC) is a phytoregulatory complex, composed of a specific family of N-heterocyclic molecules with auxin-CKs activity, selected and purified from several seaweed species. This Phytoregulatory complex has the ability to: • (i) Increase in NR // GS GOGAT Activities • (ii) Increase in Z-Pool transformation of S and C • This effect increases N transformation in proteins and aa (in shoot and root)

NP2. NS TA Process is an organo-mineral matrix formed by calcium sulphate X-hydrated and calcium sulphate tetra-ureate, where calcium atoms are complexed by humic molecules.

Technical Requirements Urée SA 21 NS TA Pre-granulator Fusion Homogeneity Reaction of N forms Matrix formation Specificity HA Resistance N-PROCESS No auto-explosion Risk Gran Drum Mill NS TA Matrix Dryer Screen Sécheur Gradual release Stabilization Screen Process Cooler Conditioning-Coating

ESTRUCTURA CRISTALINA (H 2 O) (H 2 O) (H 2 O) (H 2 O) (H 2 O) (H 2 O) (H 2 O) XO XO XO XO XO XO XO M M M M M M M M M M PCA PCA PCA PCA M M M MATRIZ ORGÁNICA HIDROFÓBICA

Main Effects: The presence of the NS TA Matrix in the fertilizer granule has two complementary effects: 1. A Physiological effect by improving N fertilization efficiency through the stabilization of the gradual simultaneous presence in the soil solution of nitrate (coming from ammonium soil nitrification), ammonium and urea: NAU effect 2. Environmental and nutritional effects derived from the control of both N soil leaching and ammonium volatilization.

 Publications

 NAU vs NA and NO 3 - Wheat Height DW Shoot DW Root DW Tillering cm g/plant g/plant plant NO 3 - 48 b 4.95 a 1.87 a 0.32 a 2.19 a NA 44.4 a 5.27 a 1.98 a 0.44 b 2.41 ab NAU 45.2 ab 6.16 b 2.22 b 0.35 a 2.57 b - (1/3) Same N dose NAU contains less NO 3 - treatment than NO 3

 NAU effect on the plant hormonal balance.

 NAU effect on the plant hormonal balance.

 NAU effect on the plant hormonal balance. CONCLUSIONS + U Enhance SHOOTS development ■ NO 3 - / NH 4 • CYTOKININS 100µM Z, tZR (MORE ACTIVE FORMS), IPR 5mM cZR, (LESS ACTIVE or INACTIVE FORMS) 100µM • IAA IAA BIOSYNTHESIS IN SHOOTS 5mM 100µM • ABA ABA BIOSYNTHESIS IN SHOOTS 5mM

NS TA Gradual Release ---- Reduction Losses

San Francisco et al., 2012 100,00 90,00 80,00 70,00 % de Nitrógeno 60,00 NProcess 29N-verde 50,00 NProcess 29N NProcess 214-10N verde 40,00 NProcess 214-10N 30,00 Nitrato de amonio 20,00 Urea 10,00 0,00 1 2 3 4 5 Lavados 6 7 8 9 10 11

Reduction in ammonium volatilization Organic sites in NS TA matrix- urea Intéraction 7 8 9 11 14 0 urée -2 -4 -6 R&D TAI and CERA -8 Study -10 -12 -14 -16 N-PROCESS -18

NP3. SEA 1. This compound is a humate- auxin precursor complex with the ability to activate soil-enzyme activities, and specifically the protease and urease soil activities. This action permits the mineralization of nitrogen from proteins- residues and amidic-residues (in general, organic nitrogen) in soil humic substances, thus increasing N soil availability for plants.

HHA-AxP

Soil Organic N Mobilization Rhizosphere - Romola Soil

N-Process action on soil-plant system  N transformation  N root uptake PMA SWC Root  N Fert - units – NAU effect  Gradual N release Rhizosphere - Matrix NS TA  Soil Microbial Activity  Soil Enzymatic activities (mainly In granule Soil Proteases and ureases ) N leaching SEA 1 N water - soil contamination SOIL  A NH3 volatilization vailable fractions of nutrients NUTRIENTS in soil, mainly inorganic nitrogen -

MPPA Duo Grassland Agro Conference R&D TAI Roullier Group JM Garcia-Mina (JGM v1 2014)

MPPA Duo A soil-plant-fertilizer catalyzer PLANTA D D FERTILIZANTE SUELO

Increase Yield and plant-soil Quality XCK Complex MPPA-Nutrients

Binding sites

Ca : Cation (Fe+++,Al+++ Acid Soils; Ca++ Calcareous Soils

II. D -N + - NH 4 - Urée

III. D -K

III. D -Oligo

Increase Yield and plant-soil Quality XCK Complex MPPA-Nutrients

Effect on plant development height Nº of Leaf d w Root d w Stem d w Plant d w Spad Trat. leaves (g) (g) (g) (g) (cm) C 41,4 17 56,0 25,7 10,7 6,6 43.1 AIA -6 46,1 19,3 77,0* 37,0* 13,6 9,7* 60.2* -3 XCK 17 43,0 14,4* 38,2* 13,2* 5,0* 3,2* 21.3* -6 XCK 17 43,4 18,5 81,0* 34,2 13,6 8,8 56.5 -9 XCK 17 42,9 20,1* 79,7* 37,0* 13,0 10,2* 60.3* * LSD for p< 0.05.