Motivation Edwin Smith Papyrus the world's oldest surviving surgical - - PowerPoint PPT Presentation

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Motivation Edwin Smith Papyrus the world's oldest surviving surgical - - PowerPoint PPT Presentation

Breast east can ance cer r ?! Motivation Edwin Smith Papyrus the world's oldest surviving surgical document. ancient Egypt around 1600 B.C. Injecting patients with a toxin ! William Coley first noticed that bacterial infections sometimes


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Edwin Smith Papyrus the world's oldest surviving surgical document. ancient Egypt around 1600 B.C.

Breast east can ance cer r ?! Motivation

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William Coley first noticed that bacterial infections sometimes diminished or even eradicated tumors more than 200 years ago.

Injecting patients with a toxin !

Coley treated over 1,000 patients with bone cancer.

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Idea!

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QS phenomena.. Power of the group

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OH baby, it’s a wild world!

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Shifts

 

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Azurin

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The Assembly- Gibson?

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Restriction enzymes at the Egyptian Market

Double digest !

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Trying

Cut with SPE1/ XbaI “fast digest “ incubation heat inactivation cutting with EcoRI/PstI ligation transformation Cut with “EcoRI/PstI incubation heat inactivation cutting with SPE1/ XbaI “fast digest ligation transformation Double digest using a Multicore buffer ligation transformation Double digest using a Tango buffer ligation transformation Double digest using the 4 enzymes “fast digest ligation transformation

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BBa_K1854001 BBa_K1854000

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iGEM EM-Ca Cair iro at at the he lab ab ?!. !.. Not tha hat eas asy y !

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Mo Moving ving mou

  • unt

ntains ains !

6- no Primers for using azurin 4- IDT !! 1- The difference between synBio & genetic engineering 2-2015 DNA distribution kit 3- the chemicals (by the start of September ) 5- the 4 enzymes at last from

  • ne company by 13th

September

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We need something dead .. Kill me some cells!

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It’s measuring time !

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Hardware

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Why ?!

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Operation Concept

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Implementation Methodology

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Challenges & Solving Approaches

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Device’s Feature

Simple

Cheap

Harmless

Instantaneous Results

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Human Practices

  

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Breast Cancer Foundation of Egypt

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Children’s University

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  

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Sum it Up !

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Attribution

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