Molecular Computing Dr. Nickolas Chelyapov Cliff Johnson Leonard - - PDF document

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Molecular Computing Dr. Nickolas Chelyapov Cliff Johnson Leonard Adleman Areio Soltani Laboratory for Molecular Rolfe Schmidt Science Billal Shaw University of Southern California Dustin Reischus Defense Advanced Research Projects Agency

SLIDE 1

Molecular Computing

Leonard Adleman Laboratory for Molecular Science University of Southern California

Dr. Nickolas Chelyapov

Cliff Johnson Areio Soltani Rolfe Schmidt Billal Shaw Dustin Reischus Defense Advanced Research Projects Agency Office of Naval Research JPL/NASA

http://americanhistory .si.edu/ve/ http://www.nen.com

SLIDE 2

DNA

http://www.uwec.edu/academic/curric /scalesjb/images/DNA/dna%20collection. htm

DNA

Extreme Information Density

http://www.uwec.edu/academic/curric /scalesjb/images/DNA/dna%20collection. htm

DNA

Extreme Information Density

http://www.uwec.edu/academic/curric /scalesjb/images/DNA/dna%20collection. htm

1 gram 4,000,000,000,000,000,000,000 bits

Polymerase

SLIDE 3

Polymerase Polymerase

Extremely Small

Polymerase

Extremely Small

1ml 5,000,000,000,000,000,000 molecules

Ligase

http://www. icnet.uk/labs/ wigley/projects/ ligase/lig1. htm

SLIDE 4

Ligase

Extremely Energy Efficient

http://www. icnet.uk/labs/ wigley/projects/ ligase/lig1. htm

Ligase

Extremely Energy Efficient

1 Joule 20,000,000,000,000,000,000

perations

http://www. icnet.uk/labs/ wigley/projects/ ligase/lig1. htm

http://www.nen.com

?

SLIDE 5

The Theory of Computation The Theory of Computation

Computing is Easy

1. A METHOD FOR STORING

INFORMATION

2. A FEW SIMPLE OPERATIONS

FOR ACTING ON INFORMATION

1. A METHOD FOR STORING

INFORMATION

2. A FEW SIMPLE OPERATIONS

FOR ACTING ON INFORMATION

Computing is Easy

1. A METHOD FOR STORING

INFORMATION

2. A FEW SIMPLE OPERATIONS

FOR ACTING ON INFORMATION

Computing is Easy

SLIDE 6

DNA COMPUTER

1994 Experiment

Satisfiability Y Y

Sue

Y N

Ted

N Y

John (B or –G) and (-R or G) and (B or G) ATCCTA TCCTAT CTCTCT ACCTCT TTAACC CAAATT

SLIDE 7

Mix and Split Synthesis

ATCCTA TCCTAT CTCTCT ACCTCT TTAACC CAAATT ATCCTA ACCTCT TCCTAT TTAACC CTCTCT CAAATT

Library Strands

ATCCTA TCCTAT CTCTCT ACCTCT TTAACC CAAATT TAGGAT AATTGG TGGAGA GTTTAA GAGAGA AGGATA

Watson-Crick Complementary Probes

SLIDE 8

CAPTURE LAYER (-R or G)

SLIDE 9

HOT

CAPTURE LAYER (-R or G)

John (B or –G) Ted (-R or G) Sue (B or G) (X_1 or -X_2 or -X_3) and (X_2 or -X_3 or - X_4) and (X_3 or -X_4 or -X_5) and (X_4 or - X_5 or -X_6) and (X_5 or -X_6 or -X_7) and (X_6 or -X_7 or -X_8) and (X_7 or -X_8 or - X_9) and (X_8 or -X_9 or -X_10) and (X_9 or

X_10 or -X_11) and (X_10 or -X_11 or -

X_12) and (X_11 or -X_12 or -X_13) and (X_14 or -X_15 or -X_16) and (X_15 or -X_16

r -X_17) and (X_16 or -X_17 or -X_18) and

(X_17 or -X_18 or -X_19) and (X_18 or -X_19

r -X_20) and (X_1 or X_2 or X_3) and (X_1
r X_2 or -X_3) and (-X_1 or X_2 or X_3)

and (-X_1 or X_2 or -X_3) and (X_1 or -X_2

r X_3)

Science, Vol. 296, Issue 5567, 499-502, April 19, 2002

300 nucs 15 nucs per variable The Library

1,048,576 distinct library strands

SLIDE 10

20 1 17 1 F T F T

Var 17

F F T T

Var 1

SLIDE 11

17 1 F T F T

Var 17

F F T T

Var 1

“It’s not that the bear dances so well, it’s that

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