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Molecular Biology, part 2 l Junk DNA l Reading frames, open reading - PowerPoint PPT Presentation

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Molecular Biology, part 2 l Junk DNA l Reading frames, open reading frames l Splicing and number of chromosomes per (somatic) cell l Prokaryotes vs. eukaryotes l Diplois and haploid cells l Different tissues express different sets of genes Number

  1. Molecular Biology, part 2 l Junk DNA l Reading frames, open reading frames l Splicing and number of chromosomes per (somatic) cell l Prokaryotes vs. eukaryotes l Diplois and haploid cells l Different tissues express different sets of genes

  2. Number of chromosomes

  3. Sequencing DNA l Length of read: 10 2 -10 3 l Length of chromosome: 10 7 -10 8 l Maps: l Genetic maps: markers are genes with “visible” effects l Physical maps: markers are “lab detectable” loci such as SSR and SNP l Sequence: the ultimate map

  4. DNA Techniques l Cloning l Cut, break, glue l Copying DNA: l Plasmid l Phage l BAC l Electrophoresis l Next generation sequencing

  5. Complete genomes l Eukaryotes: 1,939 l Prokaryotes: 31,611 l Viruses: 4,487 l Plasmids: 5,677 l Organelles: 6,368 l l Source: NCBI, 2015-02-22

  6. Databases l NCBI l GenBank l OMIM l SNP l Taxonomy l Epigenomics l UniProt l KEGG l EMBL-EBI l Ensemble

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