Probing dimerization and structural flexibility Probing dimerization - - PowerPoint PPT Presentation

▶

Sep 27, 2023 248 likes •365 views

European Molecular Biology Laboratory Hamburg Outstation European Molecular Biology Laboratory Hamburg Outstation European Molecular Biology Laboratory Hamburg Outstation European Molecular Biology Laboratory Hamburg Outstation BioSAXS group

SLIDE 1

Probing dimerization and structural flexibility Probing dimerization and structural flexibility Probing dimerization and structural flexibility Probing dimerization and structural flexibility

n mammalian lipoxygenases by SAXS
n mammalian lipoxygenases by SAXS
n mammalian lipoxygenases by SAXS
n mammalian lipoxygenases by SAXS
Dr. Weifeng Shang
Dr. Weifeng Shang
Dr. Weifeng Shang
Dr. Weifeng Shang 尚伟峰

Shang Shang Shang Shang, , , , W W W W., ., ., ., et et et et al al al al., ., ., ., J J J J. . . . Mol Mol Mol Mol. . . . Biol Biol Biol

Biol. (2011),

. (2011), . (2011), . (2011), in in in in press press press press, , , , available available available available

nline
nline
nline
nline

doi doi doi doi:10.1016/ :10.1016/ :10.1016/ :10.1016/j j j j. . . .jmb jmb jmb jmb.2011.04.035 .2011.04.035 .2011.04.035 .2011.04.035

European Molecular Biology Laboratory Hamburg Outstation European Molecular Biology Laboratory Hamburg Outstation European Molecular Biology Laboratory Hamburg Outstation European Molecular Biology Laboratory Hamburg Outstation BioSAXS group BioSAXS group BioSAXS group BioSAXS group

SLIDE 2

utline
utline
utline
utline

1 1 1 1 Introduction to Introduction to Introduction to Introduction to lipoxygenase lipoxygenase lipoxygenase lipoxygenase 2 SAXS 2 SAXS 2 SAXS 2 SAXS experiments and results experiments and results experiments and results experiments and results 3 3 3 3 Complementary techniques Complementary techniques Complementary techniques Complementary techniques

SLIDE 3

mammalian lipoxygenases (LOX) mammalian lipoxygenases (LOX) mammalian lipoxygenases (LOX) mammalian lipoxygenases (LOX) Human LOX Human LOX Human LOX Human LOX hemeostasis drug target (structure-function relationship) Crystal structure in PDB Crystal structure in PDB Crystal structure in PDB Crystal structure in PDB plant LOXs fragment of human platelet type 12S-LOX rabbit 12/15LOX (accession code:2p0m)

SLIDE 4

human and rabbit LOX human and rabbit LOX human and rabbit LOX human and rabbit LOX Oligomeric form Oligomeric form Oligomeric form Oligomeric form r-15LOX (dimer in crystal, monomer in soution) hp-12LOX (dimer in solution)

SLIDE 5

Wild type r-15LOX Wild type r-15LOX Wild type r-15LOX Wild type r-15LOX

Wild type Wild type Wild type Wild type

SLIDE 6

Y98R mutant and double mutant Y98R mutant and double mutant Y98R mutant and double mutant Y98R mutant and double mutant

SLIDE 7

Oligomerization behaviors Oligomerization behaviors Oligomerization behaviors Oligomerization behaviors

SLIDE 8

Effect of ligand Effect of ligand Effect of ligand Effect of ligand

SLIDE 9

Data reduction Data reduction Data reduction Data reduction

SLIDE 10