Lung Surfactant Derek Bacon: Santa Barbara City College, UCSB Fall - - PowerPoint PPT Presentation

Lung Surfactant

Derek Bacon: Santa Barbara City College, UCSB Fall 03, Chemistry, INSET Coralie Alonso, Professor Joe Zasadzinski Molecular Engineering Lab, Chemical Engineering

• Dept. UCSB

Funding: NIH, California State Tobacco Related Disease Program

Synthetic Lung Surfactant Protein MiniB

• Two groups of lipids

Surface pressure, and ability to Respread

• Addition of Proteins
• SP-B to MiniB

MiniB SP-B1-25

By: Dr. Alan Waring

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Brewster Angle Microscopy

Laser Camera trough Barrier Barrier Pressure Sensor

10 20 30 40 50 60 70 50 100 150 200 250 MiniB w/ Infasurf Lipids vs Infasurf Pressure mN/m TroughArea cm^2 Infasurf MiniB w/ Infa Lipids

Isotherms of Solutions with MiniB

10 20 30 40 50 60 70 50 100 150 200 250 MiniB's Effects Pressure mN/m TroughArea cm^2 Lipids MiniB 2% MiniB 10% MiniB

10 mN/m 20 mN/m 30 mN/m 40 mN/m 50 mN/m Collapse Lipids Each Picture is 400um x 400um

Images of MiniB Effects

Comparing Synthetic vs. Natural

10 mN/m 20 mN/m 30 mN/m 40 mN/m 50 mN/m Collapse Lipids + 2% MiniB (solvent) Each Picture is 400um x 400um

Conclusions

• MiniB helps the lipid mixture reach a high collapse

pressure

• MiniB helps the lipid mixture to respread
• MiniB induces more material to be at the interface
• MiniB plus the lipids a synthetic mixture mimics

natural lung surfactants well.

Special Thanks to: Tim Alig, Bill Taeusch M.D., and the rest of Joe Zasadzinski’s lab