Lung Surfactant Derek Bacon: Santa Barbara City College, UCSB Fall - PowerPoint PPT Presentation

Lung Surfactant Derek Bacon: Santa Barbara City College, UCSB Fall 03, Chemistry, INSET Coralie Alonso, Professor Joe Zasadzinski Molecular Engineering Lab, Chemical Engineering Dept. UCSB Funding: NIH, California State Tobacco Related Disease Program

Synthetic Lung Surfactant Protein MiniB SP-B 1-25 • Two groups of lipids + Surface pressure, + and ability to + + Respread + + • Addition of Proteins + + • SP-B to MiniB MiniB By: Dr. Alan Waring

Brewster Angle Microscopy Pressure Sensor Barrier Laser Camera Barrier trough

Isotherms of Solutions with MiniB MiniB's Effects MiniB w/ Infasurf Lipids vs Infasurf 70 70 Infasurf Lipids 60 60 MiniB w/ Infa Lipids MiniB 2% MiniB 50 50 10% MiniB Pressure mN/m Pressure mN/m 40 40 30 30 20 20 10 10 0 0 0 50 100 150 200 250 0 50 100 150 200 250 TroughArea cm^2 TroughArea cm^2

Images of MiniB Effects 10 mN/m 20 mN/m 30 mN/m 40 mN/m 50 mN/m Collapse Lipids Each Picture is 400um x 400um

Comparing Synthetic vs. Natural 10 mN/m 20 mN/m 30 mN/m 40 mN/m 50 mN/m Collapse Lipids + 2% MiniB (solvent) Each Picture is 400um x 400um

Conclusions • MiniB helps the lipid mixture reach a high collapse pressure • MiniB helps the lipid mixture to respread • MiniB induces more material to be at the interface • MiniB plus the lipids a synthetic mixture mimics natural lung surfactants well. Special Thanks to: Tim Alig, Bill Taeusch M.D., and the rest of Joe Zasadzinski’s lab