Issues of PRP Extraction People have different initial Red Blood Cell level. : Some PRP kits (Biomet “GPS”, Harvest “ SmartPrep ”) designed PRP extraction method in consideration of each blood has same RBC level. But, , It is not! GLO-PRP PRP (BIOMET,GPS) (Harvest, Smart Prep)
Issues of PRP Extraction (cont’d) People have different initial Platelet level in the whole blood. : A single centrifugation method is not sufficient to extract highly concentrated PLT with people who have poor PLT level. Single Spin Numbers of initial PLTs 1)A-person : 276 × 10 3 / ㎕ 2)B-person : 252 × 10 3 / ㎕ 3)C-person : 188 × 10 3 / ㎕ PLTs(10 3 ㎕ ) Time (min) Fig. Numbers of PLTs and PLT Concentration level as person at 340 × g
Issues of PRP Extraction (cont’d) Buffy Coat contains dense Platelet and white blood cell. PRP with buffy coat contained has a concentration of PLT about X4~8 . PRP without buffy coat has a concentration of PLT below X3 .(Arthrex, RegenLab) GLO-PRP RegenLab, ACR Arthrex, ACP TM You can find “GLO PRP” still These PRP kits do not take the Buffy Coat layer. contains the Buffy Coat layer that is visible in the bottom of Syringe
GLO PRP GLO PRP has all the answers. (Initial RBC&PLT differentiation / Buffy Coat / Manual pipetting / Price) GLO- PRP uses a “plunger down” method to collect PLT independent to the initial Red blood cell level. Push down the plunger!
GLO PRP (cont’d) GLO- PRP performs a “Double spin” method to separate PRP aliquot from Platelet Poor Plasma and Red Blood Cell regardless of a concentration of platelets in the whole blood. GLO PRP separates Red Blood Cell from the single spin. Then, performs the second spinning to collect highly concentrated PRP . White blood cell Platelet Red blood cell 1200g 1200g 1200g 1200g , , , , 10min 0min 5min 3min 1 st centrifugation 2 nd centrifugation
The biggest competitors in comparison to GloTech 1. closed / open system: • Advantages of closed systems: Higher sterility during procedure Less time consuming Easier preparation Finally cheaper GLO PRP: Semi Closed System
The biggest competitors in comparison to GLO PRP 2. Double soft centrifugation: • Advantages: Higher content of viable platelets Higher concentration of PRP per volume Small / large joints treatable GLO PRP:Double spin
GLO PRP • Semi-closed • Simple procedure • Low cost 8
Comparison of various PRP Kits KIT Centrifug Activator Taking Acquire Concent Cost/kit Character e method s blood d PRP rated (US $) amount amount platelet (cc) (cc) s (x) GLO PRP Double - 8.5 0.6 5.2 100* Semi-Closed Simple <Finland> procedure Low cost JP200 Double Thrombin 20 1 5.7 60 Manual CaCl2 Low cost <Japan> Magellan Double Thrombin 26-52 3-10 10.2* 600 Closed CaCl2 Full-auto <U.S.A.> Highest cost Mycells Single Thrombin 10 1-2 4.9 140 Low RBC CaCl2 Single CaCl2 250 Low RBC CellRevive 8 1-1.5 4.6 High cost 9 Dr.Shin’s PRP Single - 220 Semi-closed 8.5 1 5.2 High cost System
Recommend
More recommend
