Issues of PRP Extraction People have different initial Red Blood - - PowerPoint PPT Presentation

Issues of PRP Extraction

• People have different initial Red Blood Cell level.

: Some PRP kits (Biomet “GPS”, Harvest “SmartPrep”) designed PRP extraction

method in consideration of each blood has same RBC level. But, , It is not!

(BIOMET,GPS) (Harvest, Smart Prep)

GLO-PRP PRP

Issues of PRP Extraction (cont’d)

• People have different initial Platelet level in the whole blood.

: A single centrifugation method is not sufficient to extract highly concentrated PLT with people who have poor PLT level.

Time (min)

• Fig. Numbers of PLTs and PLT Concentration level as person at 340×g

 Numbers of initial PLTs 1)A-person : 276×103/㎕ 2)B-person : 252×103/㎕ 3)C-person : 188×103/㎕

PLTs(103㎕) Single Spin

Issues of PRP Extraction (cont’d)

• Buffy Coat contains dense Platelet and white blood cell.

 PRP with buffy coat contained has a concentration of PLT about X4~8.  PRP without buffy coat has a concentration of PLT below X3.(Arthrex, RegenLab)

You can find “GLO PRP” still contains the Buffy Coat layer that is visible in the bottom

• f Syringe

GLO-PRP

RegenLab, ACR Arthrex, ACPTM

These PRP kits do not take the Buffy Coat layer.

GLO PRP

 GLO PRP has all the answers.

(Initial RBC&PLT differentiation / Buffy Coat / Manual pipetting / Price)  GLO-PRP uses a “plunger down” method to collect PLT independent to the initial Red blood cell level.

Push down the plunger!

GLO PRP (cont’d)

 GLO-PRP performs a “Double spin” method to separate PRP aliquot from Platelet Poor Plasma and Red Blood Cell regardless of a concentration of platelets in the whole blood.

 GLO PRP separates Red Blood Cell from the single spin.  Then, performs the second spinning to collect highly concentrated PRP .

1200g , 0min

2nd centrifugation

1200g , 3min 1200g , 5min

1st centrifugation

1200g , 10min White blood cell Platelet Red blood cell

The biggest competitors in comparison to GloTech

• 1. closed / open system:
• Advantages of closed systems:
• Higher sterility during procedure
• Less time consuming
• Easier preparation
• Finally cheaper
• GLO PRP: Semi Closed System

The biggest competitors in comparison to GLO PRP

• 2. Double soft centrifugation:
• Advantages:
• Higher content of viable platelets
• Higher concentration of PRP per volume
• Small / large joints treatable
• GLO PRP:Double spin

GLO PRP

• Semi-closed
• Simple procedure
• Low cost

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KIT Centrifug e method Activator s Taking blood amount (cc) Acquire d PRP amount (cc) Concent rated platelet s (x) Cost/kit (US $) Character

GLO PRP <Finland>

Double

• 8.5

0.6 5.2 100* Semi-Closed

Simple procedure Low cost

JP200 <Japan>

Double Thrombin CaCl2

20 1 5.7 60 Manual

Low cost

Magellan <U.S.A.>

Double Thrombin CaCl2

26-52 3-10 10.2* 600 Closed

Full-auto Highest cost

Mycells

Single Thrombin CaCl2

10 1-2 4.9 140 Low RBC

CellRevive

Single CaCl2

8 1-1.5 4.6 250 Low RBC

High cost

Dr.Shin’s PRP System

Single

• 8.5

1 5.2 220 Semi-closed

High cost

Comparison of various PRP Kits

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