Introduction to Single-cell RNA-seq analysis Harvard Chan - - PowerPoint PPT Presentation
Introduction to Single-cell RNA-seq analysis Harvard Chan Bioinformatics Core https://tinyurl.com/hbc-scrnaseq-online Shannan Ho Sui John Hutchinson Victor Barrera Rory Kirchner Zhu Zhuo Director Associate Director Radhika Khetani Preetida
Rory Kirchner John Hutchinson Associate Director Victor Barrera Shannan Ho Sui Director Zhu Zhuo James Billingsley Peter Kraft Faculty Advisor Sergey Naumenko Ilya Sytchev Joon Yoon Radhika Khetani Training Director Mary Piper Meeta Mistry Jihe Liu Preetida Bhetariya
Rory Kirchner John Hutchinson Associate Director Victor Barrera Shannan Ho Sui Director Zhu Zhuo James Billingsley Peter Kraft Faculty Advisor Sergey Naumenko Ilya Sytchev Joon Yoon Radhika Khetani Training Director Mary Piper Meeta Mistry Jihe Liu Preetida Bhetariya
http://bioinformatics.sph.harvard.edu/
NIEHS
We have divided our short workshops into 2 categories:
needed (no prerequisites)
sequencing (NGS) data - Certain “Basic” workshops required as prerequisites.
Any participants wanting to take an advanced workshop will have to have taken the appropriate basic workshop(s) within the past 6 months.
http://bioinformatics.sph.harvard.edu/training/ https://hbctraining.github.io/main/
We have divided our short workshops into 2 categories:
needed (no prerequisites)
sequencing (NGS) data - Certain “Basic” workshops required as prerequisites.
Any participants wanting to take an advanced workshop will have to have taken the appropriate basic workshop(s) within the past 6 months.
http://bioinformatics.sph.harvard.edu/training/ https://hbctraining.github.io/main/
http://anoved.net/tag/lego/page/3/
✓ Describe best practices for designing a Single-cell RNA-seq
experiment
✓ Describe steps in a Single-cell RNA-seq analysis workflow. ✓ Use Seurat and associated tools to perform analysis of single-cell
expression data, including data filtering, QC, integration, clustering, and marker identification
✤ Quit/minimize all applications that are not required for class ✤ Click on “Participants” to open that panel in Zoom
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✤ Questions for the presenter?
✤ Technical difficulties with R or RStudio?