Introduction Lettuce ( Lactuca sativa L.) Romanine Buttercrunch - - PDF document

Oral Presentation CRDC 5 22/07/54 1

LOGO

Effects of polymer sizes and concentrations

• f chitosan on growth and yield of

Butterhead lettuce Lactuca sativa L. 'Butterhead' Effects of polymer sizes and concentrations

• f chitosan on growth and yield of

Butterhead lettuce Lactuca sativa L. 'Butterhead'

Presented by

Kansinee Hungsaprug Advisor: Assoc. Prof. Dr. Supachitra Chadchawan

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Introduction

Lettuce (Lactuca sativa L.)

Black seeded simpson Romanine Buttercrunch Oak leaf Red sails Sweet valentine

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Butterhead

Introduction

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Introduction

Chitosan

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deacetylation

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Chitosan Poly(2‐amino‐2‐deoxy‐D‐glucose) or poly (N‐glucosamine)

Introduction

(N glucosamine) Biopolymer D‐glucosamine (deacetylated unit) and N‐acetyl‐D‐glucosamine (acetylated unit) β‐(1,4) glycosidic linkage Oligomer and polymer g p y Degree of deacetylation 75‐95%

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Introduction

Chitosan applications Enhanced growth and productivity Prolonged fresh weight maintenance Reduced disease infection

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Introduction

Chitosan: Okra Okra treated with O80 at 25 ppm resulted in higher average height and number of leaf, flower, and pod per plant and number of leaf, flower, and pod per plant Okra treated with O80 at 50 ppm resulted in reduced okra yellow vein mosaic virus Okra treated with O80 at 20 ppm maintained fresh weight of okra pod during storage

Lotrakul et al. (2007)

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Chitosan: Dendrobium orchid Chitosan O80 at 1, 10, 50, 100 ppm induced

Introduction

• early flowering
• an increase in the accumulative inflorescence number

Chitosan P80 at 1 and 10 ppm enhanced the accumulative inflorescence number

Limpanavech et al. (2008)

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Objective

To study the influences of polymer sizes and To study the influences of polymer sizes and concentrations of chitosan on growth and yield of Butterhead lettuce

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http://www.congeladosdasonia.com.br/butterhead_lettuce1.jpg

Methods

• 1. Seed germination

10 days

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Seedlings

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Methods

• 2. Transplant

Greenhouse: Greenhouse: netted greenhouse under natural light

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Methods

• 3. Treatment: 7 treatments

control 5 ppm O8 0 1 0 ppm O8 0 2 0 ppm O8 0 5 ppm P8 0 1 0 ppm P8 0 2 0 ppm P8 0

RCBD with 3 replications of 4 plants (nutrient solution replaced weekly)

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45 days

Harvest

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head diameter f h i ht

1 2 3

Methods

68.24 g

fresh weight leaf number keep in hot‐air oven (60˚c) for 7 days

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dry weight

Fresh weight

Results

1A) 28 32 C in December

Dry weight

2A) 28 32 C i D b 1A) 28‐32°C in December

a b b a a a a 40 60 80 100 120 140

esh w eight ( g)

ab c abc ab a ab bc 2.00 3.00 4.00 5.00

ry w eight ( g)

2A) 28‐32°C in December

14 20 40 control O5 O10 O20 P5 P10 P20

Fre

0.00 1.00 control O5 O10 O20 P5 P10 P20

Dr

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Head diameter

Results

Leaf number

a bc ab bc bc c abc 23 24 25 26 27 28

d diam eter ( cm )

3A) 28‐32°C in December 4A) 28‐32°C in December

a b b b a ab ab 31 32 33 34 35 36 37

Leaf num ber

15 21 22 control O5 O10 O20 P5 P10 P20

Head

28 29 30 control O5 O10 O20 P5 P10 P20

Fresh weight

Results

Dry weight

1B) 16‐36°C in March

c a ab ab ab ab bc 40 60 80 100

esh w eight ( g)

2B) 16‐36°C in March

c bc a bc ab abc abc 1.50 2.00 2.50 3.00 3.50 4.00

y w eight ( g)

16 20 control O5 O10 O20 P5 P10 P20

Fre

0.00 0.50 1.00 control O5 O10 O20 P5 P10 P20

Dry

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Results

Leaf number Head diameter

c ab ab bc a ab ab 10 15 20 25 30

d diam eter ( cm )

3B) 16‐36°C in March 4B) 16‐36°C in March

c ab a ab a a c 29 30 31 32 33 34 35 36

Leaf num ber

17 5 control O5 O10 O20 P5 P10 P20

Head

26 27 28 control O5 O10 O20 P5 P10 P20

Discussion

Chitosan

• Enhanced growth and productivity

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(Nge et al., 2006) (El‐Sawy et al., 2010)

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Conclusion

The best productivity enhancement by chitosan was detected when 5 ppm O80 was added to the nutrient solution after growing for 45 days in the netted greenhouse under natural light. However, the temperature fluctuation in combination with chitosan application can cause negative effect on ‘Butterhead’ lettuce growth.

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Acknowledgement

• Assoc. Prof. Dr. Supachitra Chadchawan
• Assist. Prof. Dr. Kanogwan Seraypheap
• Assoc. Prof. Dr. Preeda Boon‐long
• Assist. Prof. Dr. Rath Pichyangkura

H.M. the King’s 72nd Birthday Scholarship

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LOGO

Thank you for your attention

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