Introduction of an open-source, multi-material bioprinting platform - - PowerPoint PPT Presentation

Introduction of an open-source, multi-material bioprinting platform

3D bioprinting conference

• 26 January 2016 -

Maastricht

1.CANTER at a glance 2.AM in medicine 3.Open issue 4.Why open-source? 5.Bioprinting platform 6.Research results 7.Conclusion

Overview

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

www.teamhelloworld.com

CANTER

Center for Applied Tissue Engineering and Regenerative Medicine Development of new methods and applications for tissue engineering.

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Introduction: CANTER

Knows how to build devices Makes existing things better Problem solver Understands the requirements Development of own, efficient workflow Low access barrier / no over-engineering

User

(Life Sciences)

Engineer

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Introdution: CANTER

Mechatronics Bioengineering Mechanical Engineering

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Introdution: CANTER

Clinical Sciences Biophysics Biology

Exoprosthesis

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Additive manufacturing in medicine

www.3dprintingindustry.com

Exoprosthesis

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Endoprosthesis

Additive manufacturing in medicine

www.ke-next.de

Exoprosthesis

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Endoprosthesis Bioscaffolding

Additive manufacturing in medicine

www.nationalgeographic.com

Exoprosthesis

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Endoprosthesis Bioscaffolding Biofabrication

Additive manufacturing in medicine

www.storiesbywilliams.com

Exoprosthesis

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Endoprosthesis Bioscaffolding Biofabrication

Additive manufacturing in medicine

www.storiesbywilliams.com

Open Issues

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

www.inlingua.com

• Lack of established, easy-to-use BioInks
• Huge amount of theories / ideas, that have

to be tested

• Field of basic research
• Expensive alternatives
• Fast, evolving field of additive manufacturing

• Integration of open-source hardware &

software in own projects:

• Marlin Software
• Arduino Hardware
• Great, constantly evolving community
• Low access barriers
• Simple, highly efficient interfaces

Why open-source?

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

www.wikipedia.de

Bioprinting platform

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

• Low-cost platform
• User-friendly
• Enabling a huge variety of experiments,

especially in the field of basic research

• Standard interfaces
• Highly customizable

Current development

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

• Automatic bedleveling
• Incubator unit:
• Gasparameters:
• Carbon dioxide
• Nitrogen
• Temperature
• Humidity
• LED integration
• Touchscreen interface
• Live data
• IR cam

Modularity

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

• Different usable printheads:
• Mechanically driven
• Pneumatically driven
• Different extrusionmethods:
• Mono-extrusion
• Dual-extrusion
• Different cross-linking methods:
• UV-light, green-light
• Calcium chloride
• Different printbeds:
• Petridish
• Multiwell-plate
• Non-submersive printing
• Submersive printing

Multi material

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

• Huge variety of hydrogels, regardless of

their characteristics and needs

• Different scaffold materials
• Extrusion-methods:
• Standard, single extrusion
• Mixing
• Strand next to strand
• Strand on strand
• Material enclosing

Before printing After printing Live / Dead Assay

Results

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

hMSCs Green: living cells Red: dead cells 5% (w / v) alginate hydrogel

Live / Dead Assay

Results

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

hMSCs

Live / Dead Assay Print accuracy

Results

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

hMSCs

Live / Dead Assay Print accuracy

Results

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

hMSCs

• Powerful, easy-to-use device
• Enabling a huge variety of experiments
• Ensuring critical incubation parameters

Outlook:

• Improve usability (automated slicing software)
• Release of CAD and software files

Conclusion

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

www.teamhelloworld.com

• Daniel Baier
• Julian Blaser
• Hauke Claussen-Schaumann
• Liam Daly
• Jakob Denzel
• Alfred Fuchsberger
• Max Hörter
• Benedikt Kaufmann
• Manuel Oestreich
• Tobias Petzinger
• Rainer Stabhuber
• Johannes Stepper
• Stefanie Sudhop
• Joseph Thaler

Special Thanks to…

Sascha Schwarz CANTER, Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Thank you very much for your attention!