IN INRA (F (Fran ance) e) CURE-XF Kick-off Meeting CIHEAM Bari - - PowerPoint PPT Presentation

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Pr Presentation of Pa Partner 5

IN INRA (F (Fran ance) e)

CURE-XF Kick-off Meeting CIHEAM Bari 28-29 September, 2017

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

INRA : National Institute for Agricultural Research

Ø INRA is an important French producer of publications in the agricultural sciences Ø A community of 12 000 people including 8 165 permanent staff, 1 815 full-time researchers, 2 616 interns, and 522 PhD students with INRA grants Ø 250 laboratories including 48 experimental units across 13 scientific divisions and 17 research centres Ø An annual budget of 881.57 million euros 77% of funds coming from the Ministry of Research and 20% from other publically-funded sources

CURE-XF Kick-off Meeting CIHEAM Bari 28-29 September, 2017

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Ornamentals Fruits & Vegetables Seeds IRHS = A laboratory of 235 members Researches dedicated to Quality and Health of Horticultural Crops

Resistance sustainability Architecture & Flowering Evolutive ecology Microbial communities Disease emergence Biocontrol Nutritional quality Fruit and Seed Conservation

Plant Pathogens

Genetic diversity, Genomics & Epignenomics Germination & stress Seedborne diseases

Part of a joint research unit : Horticulture and Seeds Research (IRHS) Under administ. of INRA, University of Angers and a school of engineers Agrocampus))

Researches are focused on Various scientific topics

INRA Angers Nantes center-LOCAL LEVEL

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

FRS Quasav : three federative research topics:

• Sustainable management of plant health
• Seed biology, quality and health
• Horticultural plant product qualities

Associated partner

UMR IRHS (INRA-Agrocampus Ouest-UA) EA SIFCIR (UA-USC INRA) EA SONAS (UA) EA LBPV (UN) UP GRAPPE (ESA-USC INRA) UP LEVA (ESA-USC INRA) UP EPHor (Agrocampus Ouest) Unité BVO-LSV (Anses) UE Horticole (INRA)

Main partners Equipe EGI de l’UMR IGEPP Other partners Equipe PRP de l’UR BIA GEVES Vegepolys

Federative Research Structure « Plant Quality and Health »

INRA Angers Nantes center-REGIONAL LEVEL

highly multidisciplinary network Created in 2008

Genetics, plant pathology, (eco)- physiology Phytochemistry Pest management Food processing Ecophysiology Bacteriology Plant environment Horticulture Parasitic plants Integrated biological protection Food product quality Variety and seed study and control Plant cluster

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Main action of FRS Quasav : access to

large greenhouses and confined growth chambers IMORPHEN

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Phenotyping service Sensory analysis and consumer perception

2 platforms

high-troughput phenotyping

• f seeds and plants (chloro fluo and hyperspectral cameras)

sensory analysis and consumer perception large greenhouses and confined growth chambers IMORPHEN

Main action of FRS Quasav : access to

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Phenotyping service Sensory analysis and consumer perception

2 platforms

Microorganisms collection PHYTO Phytochemicals and secondary metabolites analysis Nucleic acid analysis

ANAN

4 technical facilities

high-troughput phenotyping

• f seeds and plants

sensory analysis and consumer perception Micro-organism collection (33 public strains of Xf) large greenhouses and confined growth chambers IMORPHEN

Main action of FRS Quasav : access to

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Phenotyping service Sensory analysis and consumer perception

2 platforms

Microorganisms collection PHYTO Phytochemicals and secondary metabolites analysis Nucleic acid analysis

ANAN

4 technical facilities

high-troughput phenotyping

• f seeds and plants

sensory analysis and consumer perception Micro-organism collections (33 public strains of Xf) Microscopy Phytochemicals and secondary metabolites analysis Nucleic acid analyses large greenhouses and confined growth chambers IMORPHEN

Accomodation

Youth hostel

Main action of FRS Quasav : access to

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Projects: Ø H2020 funded project POnTE Ø H2020 funded project XF-ACTORS Ø Euphresco Promode Ø COST action EuroXanth Research activities Ø Enlarge knowledge on Xylella spp. diversity Ø Enlarge availability of Xylella spp. strains Ø Identification of sequences specific to a group of organisms expressing a given trait of interest (pathogenicity on a given host range, lineage, … ) Ø Strain surveillance, taxonomic assignation, routes of introduction and dispersion Publications Ø Jacques et al, 2016 : variants on coffee plants intercepted in France Ø Denancé et al, 2017 : subsp and STs of Xf in France

CURE-XF Kick-off Meeting CIHEAM Bari 28-29 September, 2017

Main activities on Xylella fastidiosa

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Pathogenicity tests (confined chambers) : host range sampling, symptom scoring, imaging (chlorophyll fluorescence, hyperspectral cameras) Xylem microbiome (in about 2 years) Detection/identification : conventional PCR (Minsavage et al 1994), qPCR (Harper et al 2010) genome sequencing Genetic variations : Typing : MLSA/MLST; MLVA genomic comparison (Pacbio, Illumina) development of tools (comparison, phylogeny, epidemiology…)

INRA - Our Experience on Xf

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Pathogenicity tests (confined chambers) : host range sampling, symptom scoring, imaging (chlorophyll fluorescence, hyperspectral) Xylem microbiome (in about 2 years) Detection/identification : conventional PCR (Minsavage et al 1994), qPCR (Harper et al 2010) genome sequencing Genetic variations : Typing : MLSA/MLST; MLVA genomic comparison (Pacbio, Illumina) development of tools for in silico analysis

INRA - Our Experience

WP2, Tsk2.1 (no transcriptomic) WP3, Tsk3.2 (detection Xf/Vect (no serological tools) WP4, Tsk4.1 (imaging) WP3, Tsk3.2 (NGS) WP2, Tsk2.1

Cure-Xf

WP3, Tsk3.2 (microbiome)

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Marie-Agnès Jacques Martial Briand Perrine Portier Sophie Cesbron Enora Dupas (PhD student (October 2017_October 2020)

INRA Angers-Nantes : People involved on Xf

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

INRA_Angers Nantes centre The landscape of research on plants in Angers : SFR Quasav and IRHS

A Federative Regional Research Structure on Plant Sciences since 2008 OBJECTIVE : improving plant quality in a context of sustainable agriculture MISSION : cohesion between research units through

ü Federation of all units of research in plant sciences in an unique structure ü Their Structuration around unifying themes ü The Support of these unifying themes and the development of partnerships ü the involvment in education through research ü The Sharing of facilities

Funded by the European Union H2020-MSCA-RISE-2016 CURE-XF - 734353

Federate all units of research in plant sciences in an unique structure around unifying themes

IRHS: Institute of Research on Horticulture and Seeds 60% of the human resources of SFR Quasav

Associated partner

UMR IRHS (INRA-Agrocampus Ouest-UA) EA SIFCIR (UA-USC INRA) EA SONAS (UA) EA LBPV (UN) UP GRAPPE (ESA-USC INRA) UP LEVA (ESA-USC INRA) UP EPHor (Agrocampus Ouest) Unité BVO-LSV (Anses) UE Horticole (INRA)

Main partners Equipe EGI de l’UMR IGEPP Other partners Equipe PRP de l’UR BIA GEVES Vegepolys

Federative Research Structure « Plant Quality and Health »

Genetics, plant pathology, (eco)-physiology Phytochemistry Pest management Food processing Ecophysiology Bacteriology Plant environment Horticulture Parasitic plants Integrated biological protection Food product quality Variety and seed study and control Plant cluster