SLIDE 1
N P A N P A
S S
- P
- P
AQUAPORIN
MONTPELLIER
PhD INRA-CJS 10-2010 12-2013
Karine Prado
- C. Maurel’s group
Karine Prado Karine Prado AQUAPORIN MONTPELLIER PhD INRA-CJS N A - - PowerPoint PPT Presentation
Karine Prado Karine Prado AQUAPORIN MONTPELLIER PhD INRA-CJS N A - - PowerPoint PPT Presentation
Karine Prado Karine Prado AQUAPORIN MONTPELLIER PhD INRA-CJS N A P P N A 10-2010 C. Maurels group S -P S -P 12-2013 Karine Prado EDINBURGH Post-Doc CJS 01-2014 A.J. Millars group 10-2015 AQUAPORIN MONTPELLIER PhD INRA-CJS
SLIDE 2
SLIDE 3
N P A N P A
S S
- P
- P
AQUAPORIN
EDINBURGH
Post-Doc CJS 01-2014 10-2015
MONTPELLIER
PhD INRA-CJS 10-2010 12-2013
Karine Prado
- C. Maurel’s group
A.J. Millar’s group
SLIDE 4
N P A N P A
S S
- P
- P
AQUAPORIN
EDINBURGH
Post-Doc CJS 01-2014 10-2015
MONTPELLIER
PhD INRA-CJS 10-2010 12-2013
Karine Prado
- C. Maurel’s group
A.J. Millar’s group
SLIDE 5
Ostreococcus tauri: a minimal clock system
Unicellular photosynthetic marine alga Low genetic redundancy
12.5 Mbp genome encoding ~8,000 genes
Simple cellular structure
1 mitochondrion 1 chloroplast 1 Golgi Smallest free-living eukaryote
Reduced clock Small sequenced genome Reduced signalling complexity
Reduced kinome
Conserved signalling components
Henderson et al., (2007) PlosOne , Derelle et al., (2006) PNAS , Corellou et al., (2009) Plant Cell
SLIDE 6
Ostreococcus tauri: a minimal clock system
Unicellular photosynthetic marine alga Low genetic redundancy
12.5 Mbp genome encoding ~8,000 genes
Simple cellular structure
1 mitochondrion 1 chloroplast 1 Golgi Smallest free-living eukaryote
Reduced clock Small sequenced genome Reduced signalling complexity
Reduced kinome Reduced TTFL
Conserved signalling components
Henderson et al., (2007) PlosOne , Derelle et al., (2006) PNAS , Corellou et al., (2009) Plant Cell
SLIDE 7
Ostreococcus tauri: a minimal clock system
- O. tauri is a suitable model to
identify core signalling components
- f eukaryotes
Unicellular photosynthetic marine alga Low genetic redundancy
12.5 Mbp genome encoding ~8,000 genes
Simple cellular structure
1 mitochondrion 1 chloroplast 1 Golgi Smallest free-living eukaryote
Reduced clock Small sequenced genome Reduced signalling complexity
Reduced kinome Reduced TTFL
Conserved signalling components
Henderson et al., (2007) PlosOne , Derelle et al., (2006) PNAS , Corellou et al., (2009) Plant Cell
SLIDE 8
An ancestral non-trancriptional oscillator (NTO)
Translational inhibitors
TTFL
O’Neill et al., (2011) Nature
SLIDE 9
An ancestral non-trancriptional oscillator (NTO)
Translational inhibitors
TTFL
Perodoxin
- xidation
O’Neill et al., (2011) Nature, Edgar et al., (2012) Nature
SLIDE 10
An ancestral non-trancriptional oscillator (NTO)
Translational inhibitors
TTFL NTO
Perodoxin
- xidation
COUPLING
O’Neill et al., (2011) Nature, Edgar et al., (2012) Nature
SLIDE 11
An ancestral non-trancriptional oscillator (NTO)
Translational inhibitors
TTFL NTO
Perodoxin
- xidation
COUPLING
O’Neill et al., (2011) Nature, Edgar et al., (2012) Nature
SLIDE 12
An ancestral non-trancriptional oscillator (NTO)
Translational inhibitors
TTFL NTO
Perodoxin
- xidation
COUPLING
Ancestral NTO requires cell signalling processes including protein kinases Signalling
?
O’Neill et al., (2011) Nature, Edgar et al., (2012) Nature
SLIDE 13
An ancestral non-trancriptional oscillator (NTO)
Translational inhibitors
TTFL NTO
Perodoxin
- xidation
COUPLING
Signalling
?
Clock components
- Time resolved
phosphoproteomics
- Pharmacological
clock modulation
? ? ?
Ancestral NTO requires cell signalling processes including protein kinases
O’Neill et al., (2011) Nature, Edgar et al., (2012) Nature
SLIDE 14