How Polyubiquitin Chains are Made and Unmade Dr. Irwin A. Rose - - PowerPoint PPT Presentation

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May 16, 2023 156 likes •248 views

How Polyubiquitin Chains are Made and Unmade Dr. Irwin A. Rose University of California, Irvine Intermediates in Ub Activation by E1 The Sequence and Distribution of Enzyme Intermediates (Haas & Rose, 1982) How a protein may become

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How Polyubiquitin Chains are Made and Unmade

Dr. Irwin A. Rose

University of California, Irvine

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Intermediates in Ub Activation by E1

The Sequence and Distribution of Enzyme Intermediates (Haas & Rose, 1982)

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How a protein may become polyubiquinate?

(Alberts, The Cell, p. 360)

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Test of E1 as a Continuous Ub Source

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Ubiquitin Carboxy-terminal Hydrolase

Ub + E1SH

ES Ub ATP AMP + PPi GSH (New Enzyme)

GSH

transthiolation GS-Ub (Spon.)

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