H. glaberrima intestinal mesentery structure Sonya J. - PowerPoint PPT Presentation

Dec 01, 2022 •172 likes •336 views

An immunohistochemical approach to H. glaberrima intestinal mesentery structure Sonya J. Malavez-Cajigas, Christian J. Nieves-Ros, Samuel A. lvarez-Falcn & Jos E. Garca-Arrars Mesentery Maintains organs in place

An immunohistochemical approach to H. glaberrima intestinal mesentery structure Sonya J. Malavez-Cajigas, Christian J. Nieves-Ríos, Samuel A. Álvarez-Falcón & José E. García-Arrarás
Mesentery • Maintains organs in place • Mesothelium and connective tissue • Very important for intestine regeneration
Previous work
Objective Establish the location and arrangement of the different tissue layers and nerve plexus in the intestinal mesentery.
Method Tissue Immunohisto Tissue was containing Animals were chemistry sectioned in collected and large intestine was cryostat sacrificed. mesentery was performed . (20µm). obtained.
Antibodies and markers • Meso I- mesothelium • Phalloidin- muscle • RN1- nerve • GFS- nerve subpopulation • Collagen (IBA2)- basal lamina • DAPI- nuclei
Double labeling Meso I RN1 IBA2 IBA2 Phalloidin Phalloidin GFS Phalloidin
Results
Meso I + Phalloidin Phall Phall Meso I Meso I Dapi Dapi 20x 40x
RN1 + Phalloidin Phall Phall RN1 RN1 Dapi Dapi 20x 40x
IBA 2 + Phalloidin Phall Phall IBA2 IBA2 Dapi Dapi 20x 40x
IBA2 + GFS GFS IBA2 Dapi 20x
Conclusions The intestinal mesentery has the following tissue arrangement: Celomic epithelium Muscle Basal lamina Nervous tissue Connective tissue
Acknowledgements • Lymarie Díaz & Miosotis Alicea • UPRRP • Developmental Neurobiology Laboratory • NeuroID

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