Giant Jamboree Boston 10/31/14 Pollution Environmental Health - - PowerPoint PPT Presentation

giant jamboree boston 10 31 14 pollution environmental
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Giant Jamboree Boston 10/31/14 Pollution Environmental Health - - PowerPoint PPT Presentation

Giant Jamboree Boston 10/31/14 Pollution Environmental Health Problems (Reference: The Drive Behind Mission #1 Plastic Pollution. Retrieved from http://www.everythingconnects.org/plastic-pollution.html Web site of digit8 design. Retrieved


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Giant Jamboree Boston 10/31/14

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Pollution Environmental

Health Problems

(Reference: The Drive Behind Mission #1 Plastic Pollution. Retrieved from http://www.everythingconnects.org/plastic-pollution.html Web site of digit8 design. Retrieved from http://gogogloglo.wordpress.com/2010/11/03/national-geographic-water-the-source-of-life/ Website of National Geographic. Retrieved from http://education.nationalgeographic.com/education/encyclopedia/great-pacific- garbage-patch/?ar_a=1 )

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Degredo PETronum !

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Association of Postconsumer Plastics Recyclers (APR)

Plastic PET Production

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Association of Postconsumer Plastics Recyclers (APR)

Plastic PET Production

  • 265 Mtone produced each year
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PLASTIC PRODUCTION / PLASTIC RECYCLING

HYPERTENSION DIAGNOSTICS INC

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Disposal, Recycling and Energy Recovery 2012

Pyruvate

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Why not synthetic?

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PET Chemical Structure

PET consists polymerized units

  • f the monomer ethylene

terephthalate, with repeating C10H8O4 units

(Reference: Polyethylene terephthalate. Wikipedia. Retrieved from http://en.wikipedia.org/wiki/Polyethylene_terephthalate )

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2010 London Imperial College 2012 UC_Davis 2012 TU Darmstadt 2014 METU iGEM PET

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Kanahisa Laboratories

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Kanahisa Laboratories

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Structural Analysis

Blanka Vrchotová, Martina Macková, Tomáš Macek and Kateřina Demnerová (2013). Bioremediation of Chlorobenzoic Acids, Applied Bioremediation - Active and Passive Approaches, Dr. Yogesh Patil (Ed.), ISBN: 978-953-51-1200-6, InTech, DOI: 10.5772/56394. Available from: http://www.intechopen.com/books/applied- bioremediation-active-and-passive-approaches/bioremediation-of-chlorobenzoic-acids

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PET- Pyruvate Pathways

http://2014.igem.org/Team:METU_Turkey_project

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J23108 K316003 K1095000 K1392931 K1392932 B1006 C.Promoter RBS+Catechol Hydrolase Hydratase Aldolase Terminator 2,3-dioxygenase

Our Circuit Design

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New part Bba_K1392931

2-oxopent-4-enoate hydratase Escherichia Coli BL21(DE3) 2-oxopent-4-enoate + H2O  4-hydroxy-2-oxopentanoate

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New part Bba_K1392932

4-hydroxy-2-oxoglutarate pyruvate + glyoxylate 4-hydroxy-2-oxoglutarate aldolase Escherichia Coli BL21(DE3)

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Characterization of Catechol-2,3- dioxygenase

K1392991 = J23108 + K316003

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Kill-Switch

K80800 K515004 C0040 B1006 AraC RBS+Antiholin Tet+LVA Terminator R0040 K112806 K124014 B1006 TetR T4 Endolysin Holin Terminator

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The issue

Arabinose present Arabinose absent

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MODELING

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MODELING

Catechol

Catechol 2-3 2-hydroxymuconate 2-hydroxymuconate dioxygenase semialdehyte semialdehyte hydrolase 4-hydroxy 2-oxopent 4-enoate 2-oxopent 2-oxopentaonate hydratase 4-enoate 4-hydroxy 2-oxovalerate aldolase Pyruvate

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Catechol Degredation

  • Simulation of catechol diffuses by cell and degrades to

pyruvate instantly

http://2014.igem.org/Team:METU_Turkey_modeling

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Parameters of Catechol Degredation

Parameters of our simulation which we used in catechol degradation to pyruvate

http://parts.igem.org/wiki/index.php?title=Part:BBa_K1392991

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Characterization

Characterization of our Bba_K1392991 part by exposing catechol

Adding K1392991

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Graphs of Kill Switch Model

1)Presence of arabinose the simulation of AraC promoter without holin and endolysin

http://2014.igem.org/Team:METU_Turkey_modeling

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2) Absence of Arabinose, the simulation of cell lysis by holin and endolysin

Graphs of Kill Switch Model

http://2014.igem.org/Team:METU_Turkey_modeling

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Parameters of Kill Switch Model

Parameters of our kill switch model bu using AraC and TetR promoter

http://2011.igem.org/Team:St_Andrews/modelling http://2011.igem.org/Team:ETH_Zurich/Modeling/Parameters

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Achievements

2 New Parts added to the libary

  • K1392931
  • K1392932

Composite and Characterization

  • K1392991

Composite Parts added to the libary

  • K1392971
  • K1392972
  • K1392939
  • K1392938
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InterLab Study

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First Device Second Device Third Device

2) BBa_J23101 + BBa_E0240 in pSB1C3 backbone 3) BBa_J23115 + BBa_E0240 in pSB1C3 backbone 1) BBa_I20260 in the pSB3K3 vector

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POLICY & PRACTICE

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SynBio Survey!

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  • II. METU The Day of Synthetic Biology!
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PET Degradation Game

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METU Development Foundation Schools

  • We gave Lab education to young iGEM'ers!
  • We have started, advised and helped to this year's

High School Team "METUHS-Ankara"!

  • We are raising new iGEM'ers!
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PET Degredation Meeting in Eskişehir!

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Turkey iGEM Teams Meeting

08/06/14 Ankara,Turkey

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SynBio Party! #2 SyBio Party! #3

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Future Prospect

Genetic Modified Organism

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Thank you for your attention

Special thanks to :

  • Prof. Dr. Mahinur S. Akkaya
  • Assoc. Prof Dr. Çağdaş D. Son
  • Assoc. Prof Dr. Mayda Gürsel

R.A Ayça Çırçır R.A Çiğdem Yılmaz R.A Handan Melike Dönertaş R.A Side Selin Su Yirmibeşoğlu R.A Alişan Kayabölen CEO Burak Yılmaz

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Sponsors

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Team

Visit our wiki !

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Special thanks to :

  • Prof. Dr. Mahinur S. Akkaya
  • Assoc. Prof Dr. Çağdaş D. Son
  • Assoc. Prof Dr. Mayda Gürsel

R.A Ayça Çırçır R.A Çiğdem Yılmaz Handan Melike Dönertaş Side Selin Su Yirmibeşoğlu Alişan Kayabölen CEO Burak Yılmaz