Giant Jamboree Boston 10/31/14
Giant Jamboree Boston 10/31/14 Pollution Environmental Health - - PowerPoint PPT Presentation
Giant Jamboree Boston 10/31/14 Pollution Environmental Health - - PowerPoint PPT Presentation
Giant Jamboree Boston 10/31/14 Pollution Environmental Health Problems (Reference: The Drive Behind Mission #1 Plastic Pollution. Retrieved from http://www.everythingconnects.org/plastic-pollution.html Web site of digit8 design. Retrieved
Pollution Environmental
Health Problems
(Reference: The Drive Behind Mission #1 Plastic Pollution. Retrieved from http://www.everythingconnects.org/plastic-pollution.html Web site of digit8 design. Retrieved from http://gogogloglo.wordpress.com/2010/11/03/national-geographic-water-the-source-of-life/ Website of National Geographic. Retrieved from http://education.nationalgeographic.com/education/encyclopedia/great-pacific- garbage-patch/?ar_a=1 )
Degredo PETronum !
Association of Postconsumer Plastics Recyclers (APR)
Plastic PET Production
Association of Postconsumer Plastics Recyclers (APR)
Plastic PET Production
- 265 Mtone produced each year
PLASTIC PRODUCTION / PLASTIC RECYCLING
HYPERTENSION DIAGNOSTICS INC
Disposal, Recycling and Energy Recovery 2012
Pyruvate
Why not synthetic?
PET Chemical Structure
PET consists polymerized units
- f the monomer ethylene
terephthalate, with repeating C10H8O4 units
(Reference: Polyethylene terephthalate. Wikipedia. Retrieved from http://en.wikipedia.org/wiki/Polyethylene_terephthalate )
2010 London Imperial College 2012 UC_Davis 2012 TU Darmstadt 2014 METU iGEM PET
Kanahisa Laboratories
Kanahisa Laboratories
Structural Analysis
Blanka Vrchotová, Martina Macková, Tomáš Macek and Kateřina Demnerová (2013). Bioremediation of Chlorobenzoic Acids, Applied Bioremediation - Active and Passive Approaches, Dr. Yogesh Patil (Ed.), ISBN: 978-953-51-1200-6, InTech, DOI: 10.5772/56394. Available from: http://www.intechopen.com/books/applied- bioremediation-active-and-passive-approaches/bioremediation-of-chlorobenzoic-acids
PET- Pyruvate Pathways
http://2014.igem.org/Team:METU_Turkey_project
J23108 K316003 K1095000 K1392931 K1392932 B1006 C.Promoter RBS+Catechol Hydrolase Hydratase Aldolase Terminator 2,3-dioxygenase
Our Circuit Design
New part Bba_K1392931
2-oxopent-4-enoate hydratase Escherichia Coli BL21(DE3) 2-oxopent-4-enoate + H2O 4-hydroxy-2-oxopentanoate
New part Bba_K1392932
4-hydroxy-2-oxoglutarate pyruvate + glyoxylate 4-hydroxy-2-oxoglutarate aldolase Escherichia Coli BL21(DE3)
Characterization of Catechol-2,3- dioxygenase
K1392991 = J23108 + K316003
Kill-Switch
K80800 K515004 C0040 B1006 AraC RBS+Antiholin Tet+LVA Terminator R0040 K112806 K124014 B1006 TetR T4 Endolysin Holin Terminator
The issue
Arabinose present Arabinose absent
MODELING
MODELING
Catechol
Catechol 2-3 2-hydroxymuconate 2-hydroxymuconate dioxygenase semialdehyte semialdehyte hydrolase 4-hydroxy 2-oxopent 4-enoate 2-oxopent 2-oxopentaonate hydratase 4-enoate 4-hydroxy 2-oxovalerate aldolase Pyruvate
Catechol Degredation
- Simulation of catechol diffuses by cell and degrades to
pyruvate instantly
http://2014.igem.org/Team:METU_Turkey_modeling
Parameters of Catechol Degredation
Parameters of our simulation which we used in catechol degradation to pyruvate
http://parts.igem.org/wiki/index.php?title=Part:BBa_K1392991
Characterization
Characterization of our Bba_K1392991 part by exposing catechol
Adding K1392991
Graphs of Kill Switch Model
1)Presence of arabinose the simulation of AraC promoter without holin and endolysin
http://2014.igem.org/Team:METU_Turkey_modeling
2) Absence of Arabinose, the simulation of cell lysis by holin and endolysin
Graphs of Kill Switch Model
http://2014.igem.org/Team:METU_Turkey_modeling
Parameters of Kill Switch Model
Parameters of our kill switch model bu using AraC and TetR promoter
http://2011.igem.org/Team:St_Andrews/modelling http://2011.igem.org/Team:ETH_Zurich/Modeling/Parameters
Achievements
2 New Parts added to the libary
- K1392931
- K1392932
Composite and Characterization
- K1392991
Composite Parts added to the libary
- K1392971
- K1392972
- K1392939
- K1392938
InterLab Study
First Device Second Device Third Device
2) BBa_J23101 + BBa_E0240 in pSB1C3 backbone 3) BBa_J23115 + BBa_E0240 in pSB1C3 backbone 1) BBa_I20260 in the pSB3K3 vector
POLICY & PRACTICE
SynBio Survey!
- II. METU The Day of Synthetic Biology!
PET Degradation Game
METU Development Foundation Schools
- We gave Lab education to young iGEM'ers!
- We have started, advised and helped to this year's
High School Team "METUHS-Ankara"!
- We are raising new iGEM'ers!
PET Degredation Meeting in Eskişehir!
Turkey iGEM Teams Meeting
08/06/14 Ankara,Turkey
SynBio Party! #2 SyBio Party! #3
Future Prospect
Genetic Modified Organism
Thank you for your attention
Special thanks to :
- Prof. Dr. Mahinur S. Akkaya
- Assoc. Prof Dr. Çağdaş D. Son
- Assoc. Prof Dr. Mayda Gürsel
R.A Ayça Çırçır R.A Çiğdem Yılmaz R.A Handan Melike Dönertaş R.A Side Selin Su Yirmibeşoğlu R.A Alişan Kayabölen CEO Burak Yılmaz
Sponsors
Team
Visit our wiki !
Special thanks to :
- Prof. Dr. Mahinur S. Akkaya
- Assoc. Prof Dr. Çağdaş D. Son
- Assoc. Prof Dr. Mayda Gürsel
R.A Ayça Çırçır R.A Çiğdem Yılmaz Handan Melike Dönertaş Side Selin Su Yirmibeşoğlu Alişan Kayabölen CEO Burak Yılmaz