Missouri Miners 2014 Giant Jamboree Burning fossil fuels releases - - PowerPoint PPT Presentation

▶

Sep 16, 2022 433 likes •660 views

Missouri Miners 2014 Giant Jamboree Burning fossil fuels releases flue gases Contain many pollutants Microbes can utilize http://www.nma.org/index.php/coal-statistics Impact of coal emissions per year in the U.S. (Schneider and

SLIDE 1

SLIDE 2

Missouri Miners 2014 Giant Jamboree

SLIDE 3

SLIDE 4

 Burning fossil fuels releases

flue gases

 Contain many pollutants  Microbes can utilize

SLIDE 5

http://www.nma.org/index.php/coal-statistics

SLIDE 6

SLIDE 7

 Impact of coal emissions per year

in the U.S. (Schneider and Banks, 2010)

13,000

,000 premature deaths

20,00 ,000 heart attacks

6 million llion lost workdays

 Pollutants need to be filtered

effectively and economically

Missouri S&T Coal Plant

SLIDE 8

 Lots of specialized equipment  Each pollutant has a different

method of removal

 Proper disposal of byproducts

http://i.huffpost.com/gen/1380970/thumbs/o-COAL- POWER-PLANT-facebook.jpg

SLIDE 9

 Create and identify preexisting BioBricks  Express parts in photosynthetic organism  Incorporate organism into bioreactor

NO NOx

SLIDE 10

SLIDE 11

http://www.ecs.umass.edu/che/henson_group/research/bioreactor.html

SLIDE 12

N2O

SLIDE 13

 E. coli K12

norV – oxidoreductase
Hmp – nitric oxide deoxygenase
nrfA – nitrite reductase

 P. aeruginosa PAO1

norCB – nitric oxide reductase
nosZ – nitrous oxide reductase

SLIDE 14

So Far Still in Progress

 Bba_K113001 - nrfA  Bba_K896005 – norCB

Bba_K896004 – nosZ and norCB

together

 Bba_K1356006 – nosZ  Bba_K1

_K1370000 - hmp

 norV part

Internal PstI site removed
Ligation trouble

 nosZ part

Amplification troubles
Internal site needs removal

SLIDE 15

 Further characterize hmp  Continue work on nosZ and norV  Functionalize genes  Test in bioreactor

SLIDE 16

SLIDE 17

 Student Design and Experiential

Learning Center (SDELC)

 Prestigious group of student-

led, competitive design teams

 We are the only biological

engineering design team on our campus What’s coming up:

 New lab space within the Center  Building corporate partnerships  Promot

moting

ing the SDELC LC visi sion

n of

student dent succe ccess ss

SLIDE 18

SLIDE 19

SLIDE 20

 Exploring Synthetic

Biology

 Play with DNA!

Residential Life: Midwest

Regional Program of the Month Award

 Speak Up Speak Out  Waynesville High School

STEM Expo

 Engineers’ Week at St.

Louis Science Center

 And many more!

SLIDE 21

 Cruz-Ramos, H., Crack, J., Wu, G., Hughes, M. N., Scott, C., Thomson, A. J., ... &

Poole, R. K. (2002). NO sensing by FNR: regulation of the Escherichia coli NO- detoxifying flavohaemoglobin, Hmp. The EMBO journal, 21(13), 3235-3244.

 Gardner, P. R., Gardner, A. M., Martin, L. A., & Salzman, A. L. (1998). Nitric

xide dioxygenase: an enzymic function for flavohemoglobin. Proceedings of

the National Academy of Sciences, 95(18), 10378-10383.

 Hausladen, A., Gow, A. J., & Stamler, J. S. (1998). Nitrosative stress: metabolic

pathway involving the flavohemoglobin. Proceedings of the National Academy

f Sciences, 95(24), 14100-14105.

 Hmp fused nitric oxide dioxygenase/dihydropteridine reductase 2. (n.d.).

Retrieved from http://www.ncbi.nlm.nih.gov/gene/947018

 Schneider, C., and Jonathan Banks. 2010. The Toll From Coal: An Updated

Assessment of Death and Disease from America’s Dirtiest Energy Source. Clean Air Task Force, September.

SLIDE 22

 Our advisors, Dr. Westenberg & Dr. Shannon  Missouri S&T SDELC  Missouri S&T Department of Biological

Sciences

 Missouri S&T Department of Chemistry  Missouri S&T Department of Chemical

Engineering

 Missouri S&T cDNA Center  Donor Fred Kielhorn  Former team members Alie Abele and Blythe

Ferriere