Fuel for the Future Annakarin Petra Linda Riikka Tamannae - - PowerPoint PPT Presentation

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fuel for the future annakarin petra linda riikka tamannae
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Fuel for the Future Annakarin Petra Linda Riikka Tamannae - - PowerPoint PPT Presentation

May 23, 2023 127 likes •612 views

Fuel for the Future Annakarin Petra Linda Riikka Tamannae Milla-Mari Victor Arto Juuso Tuukka CO 2 Widely used in rural areas Vehicle conversion possible Existing distribution network Inexpensive technology Liquefaction requires

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Fuel for the Future

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Tamannae Annakarin Victor Arto Juuso Tuukka Petra Linda Riikka Milla-Mari

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CO2

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Existing distribution network Liquefaction requires little energy Vehicle conversion possible Widely used in rural areas Inexpensive technology

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3 enzymes hydrolyze cellulose into glucose

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The glucose is taken up by the bacteria

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Propane is produced inside the cell

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AtoB Hbd YciA Crt Ter Sfp ADO CARapo Fpr

NADPH NADP+ Acetyl-CoA Acetoacetyl-CoA 3-Hydroxybutyryl-CoA Butyryl-CoA Butyrate Crotonyl-CoA Butyraldehyde

CARholo

PetFred PetFox

Propane

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Propane 1 Propane 2

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Cellulose

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AtoB FadB2 Ter Crt YciA CAR ADO

O CoA O CoA O O CoA OH O CoA O CoA O OH O H

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AtoB FadB2 Ter Crt YciA CAR ADO

O CoA O CoA O O CoA OH O CoA O CoA O OH O H

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AtoB Hbd Ter Crt YciA CAR ADO

O CoA O CoA O O CoA OH O CoA O CoA O OH O H

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AtoB Hbd Ter Crt YciA CAR ADO

O CoA O O CoA OH O CoA O CoA O OH O H O CoA

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CAR ADO Competing enzymes

O OH O

Butyrate Butyraldehyde Propane Useless side products

H

Toxic

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ADO CAR

Hydrophilic Hydrophobic

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Propane 1 Propane 2 Cellulose Fusable GFP Amphiphilic

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E.coli BL21 (DE3 ΔyjgB ΔyqhD, pET-TPC4 + pCDF- cAD + pACYC-Fdx-Fpr) made by Kallio et al.. 4.5 & 22.7µg/l

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100 % 60 % 20 % 0 %

iGEM 2015 n=39

Biology Biotechnology Mathematical Chemistry Other

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100 % 60 % 20 % 0 %

iGEM 2014 n=1063

Biology Mathematical Chemistry Other

iGEM 2015 n=39

Biology Biotechnology Mathematical Chemistry Other Biotechnology

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100 % 60 % 20 % 0 %

Professionals n=64

Biology Biotechnology Mathematical Chemistry Other

iGEM 2014 n=1063

Biology Biotechnology Mathematical Chemistry Other

iGEM 2015 n=39

Biology Biotechnology Mathematical Chemistry Other

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University of Turku Tampere University of Technology Viikki High School Mäkelänrinne High School Science Picnic Biology Olympiads

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http://cs.aaltohelsinki.com/

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http://cs.aaltohelsinki.com/

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  • A stochastic model written

in Python

  • Models enzymes and

substrates as particles in a 2D space

  • Simplified enzyme kinetics
  • Highly flexible settings
  • Compares enzymes near

each other to freely moving enzymes

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Fusable GFP

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E.coli BL21 (DE3 ΔyjgB ΔyqhD, pET-TPC4 + pCDF- cAD + pACYC-Fdx-Fpr) made by Kallio et al.. 4.5 & 22.7µg/l

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Propane 1 Propane 2 Cellulose