Fuel for the Future
Annakarin Petra Linda Riikka Tamannae Milla-Mari Victor Arto Juuso Tuukka
CO 2
Widely used in rural areas Vehicle conversion possible Existing distribution network Inexpensive technology Liquefaction requires little energy
3 enzymes hydrolyze cellulose into glucose
The glucose is taken up by the bacteria
Propane is produced inside the cell
AtoB Hbd Crt Ter Acetyl-CoA Acetoacetyl-CoA 3-Hydroxybutyryl-CoA Crotonyl-CoA CAR apo NADPH Fpr PetF red Sfp NADP + YciA PetF ox CAR holo ADO Butyryl-CoA Butyrate Butyraldehyde Propane
Propane 1 Propane 2
Cellulose
O CoA O O AtoB CoA OH O FadB2 CoA Ter O CoA Crt O CoA YciA O OH CAR O H ADO
O CoA O O AtoB CoA OH O FadB2 CoA Ter O CoA Crt O CoA YciA O OH CAR O H ADO
O CoA O O AtoB CoA OH O Hbd CoA Ter O CoA Crt O CoA YciA O OH CAR O H ADO
O CoA O O AtoB CoA OH O Hbd CoA Ter O CoA Crt O CoA YciA O OH CAR O H ADO
Toxic O O OH H ADO Butyrate CAR Butyraldehyde Propane Competing enzymes Useless side products
Hydrophilic Hydrophobic ADO CAR
Propane 1 Propane 2 Cellulose Amphiphilic Fusable GFP
4.5 & 22.7µg/l E.coli BL21 (DE3 Δ yjgB Δ yqhD, pET-TPC4 + pCDF- cAD + pACYC-Fdx-Fpr) made by Kallio et al. .
100 % Other Chemistry Mathematical Biotechnology 60 % Biology 20 % 0 % iGEM 2015 n=39
Other 100 % Other Chemistry Chemistry Mathematical Mathematical Biotechnology Biotechnology 60 % Biology Biology 20 % 0 % iGEM 2015 iGEM 2014 n=39 n=1063
Other 100 % Other Other Chemistry Chemistry Chemistry Mathematical Mathematical Mathematical Biotechnology Biotechnology 60 % Biotechnology Biology Biology Biology 20 % 0 % iGEM 2015 iGEM 2014 Professionals n=39 n=1063 n=64
University of Turku Science Picnic Viikki High School Tampere University of Technology Biology Olympiads Mäkelänrinne High School
http://cs.aaltohelsinki.com/
http://cs.aaltohelsinki.com/
• • • • • • A stochastic model written in Python • Models enzymes and substrates as particles in a 2D space • Simplified enzyme kinetics • Highly flexible settings • Compares enzymes near each other to freely moving enzymes
• • • • •
Fusable GFP
4.5 & 22.7µg/l E.coli BL21 (DE3 Δ yjgB Δ yqhD, pET-TPC4 + pCDF- cAD + pACYC-Fdx-Fpr) made by Kallio et al. .
Propane 1 Propane 2 Cellulose
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