from Lippia salsa Griseb. (Verbenaceae) Florencia Antonella Musso, - - PowerPoint PPT Presentation

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Oct 26, 2022 306 likes •385 views

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae) Florencia Antonella Musso, Valeria Cavallaro and Ana Paula Murray I N Q U I S U R - C O N I C E T , D E P A R T A M E N T O D E Q U M I C A , U N I V E R S I D A D N

SLIDE 1

I N Q U I S U R - C O N I C E T , D E P A R T A M E N T O D E Q U Í M I C A , U N I V E R S I D A D N A C I O N A L D E L S U R , A V D A . A L E M 1 2 5 3 , B A H Í A B L A N C A , A R G E N T I N A . E - M A I L : a p m u r r a y @ u n s . e d u . a r

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae)

Florencia Antonella Musso, Valeria Cavallaro and Ana Paula Murray

SLIDE 2

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae)

Lippia salsa (Verbenaceae) endemic species from Argentina

SLIDE 3

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae)

Ethanolic extrac + AChE inhibition IC50 = 0.89 mg/mL

SLIDE 4

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae)

Liquid-liquid extraction

Ethanolic extrac suspended in H2O EtOAc sub-extrac + AChE inhibition (40 % inhibition at 0.45 mg/mL)

SLIDE 5

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae)

Bioassay-guided

chromatographic separation of active sub-extract

Silica gel 60 hexane:EtOAc as eluent Fractions 14-16 (110 mg) were the most active with 79.2 % of AChE inhibition at 0.45 mg/mL Sephadex LH20 MeOH as eluent

SLIDE 6

Bioactive secondary metabolites from Lippia salsa Griseb. (Verbenaceae)

Bioassay-guided purification of active fractions

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