Fluorescent Dyes and Reporters Akita Innovations 6/9/2015 1 - - PowerPoint PPT Presentation

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Fluorescent Dyes and Reporters Akita Innovations 6/9/2015 1 - - PowerPoint PPT Presentation

Nov 10, 2022 355 likes •460 views

www.akitainnovations.com Fluorescent Dyes and Reporters Akita Innovations 6/9/2015 1 Expertise Akita Innovations performs research and product development for U.S. government and commercial customers. Akitas expertise includes synthesis

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Fluorescent Dyes and Reporters

Akita Innovations

1 6/9/2015

www.akitainnovations.com

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Expertise

Akita Innovations performs research and product development for U.S. government and commercial

  • customers. Akita’s expertise includes synthesis of

small molecules and polymers, membranes & coatings, chemicals and materials with special optical absorbance and luminescence, as well as chemical and biochemical sensors. We perform scale-up and production from mg to kg levels.

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In Billerica, MA; equipped for chemical synthesis and chemical & optical characterization

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Customers & partners

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Absorbing & fluorescing dyes

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Dyes are organic and organometallic (structures proprietary)

0.3% Triton X-100 water CH2Cl2

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Particular experience with NIR Dyes

  • Emit in ‘physiological window’ in NIR
  • Easily excited by powerful, inexpensive NIR lasers
  • Low fluorescence yield (energy gap law) an issue
  • Large Stoke’s shifts possible, challenging in NIR

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Fluorescence (blue) and excitation (red) spectra of a naphthalocyanine dye in methanol Thiophene- based dye

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Dye modifications to shift, enhance,

  • r quench fluorescence

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Example of 532 nm absorbing dyes designed for high fluorescence (red) or minimal fluorescence (green), in injection-molded polycarbonate

Index #

lmax (nm)

456 800 594 810 638 818 493 830 489 838 669 850 573 861 350 871 582 880 581 890 609 900 428 911 592 918 501 945 335 953

Example narrow-band NIR absorber dye wavelengths

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SLIDE 8

Absorbers (high VLT) also made

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Using CIE1931 Illuminant C; PLT is photopic (daytime) transmittance, SLT is scotopic (dark-adjusted eye) transmittance; spectra scaled to 532 nm absorbance of 4.0 (left) or 785 nm absorbance of 3.0 (right)

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Analyte Sensitive Fluorophores

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  • pH
  • Oxygen
  • Ions
  • Explosives
  • Chemical agents

Nitroaromatic explosive-sensitive conjugated polymer manufactured by Akita team (MIT invented, licensed and marketed by FLIR Systems) No explosive  with explosive DVDs containing oxygen-reactive laser-absorbing dyes Dye developed by Akita personnel

Data courtesy of W. Di and Prof. Heather Clark, Northeastern University

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Dyes in/on media

  • Micro/nanospheres (polymer, silica)
  • Thin polymer films
  • Membranes
  • Covalently linked to polymers
  • Bulk plastic (injection molded,

extruded)

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