SLIDE 1 Value of Researching Fluorescent Silver Nanoclusters
http://www.nature.com/scitable/blog/watching-the-detectives/how_will_hiv_affect_human
Rapid and affordable DNA detection
SLIDE 2 A Novel Approach: Fluorescent Silver Nanoclusters
DNA Probe Silver Salt and Target DNA Reducing Agent Silver Nanoclusters
Image Courtesy of Travis O’Donnell
SLIDE 3 Target DNA
Ag nanocluster region
Detecting DNA with Silver Nanoclusters
DNA Probe
Silver Salt Reducing Agent
Image Courtesy of Travis O’Donnell
SLIDE 4
A Simple DNA Test
No Hepatitis DNA Silver Salt Hepatitis DNA Sodium Phosphate Reducing Agent
SLIDE 5 Fluorescence Emission Spectrum
With Hepatitis DNA Without Hepatitis DNA 625nm
Hepatitis B
Wavelength (nm) Intensity (cts/sec)
SLIDE 6 With Hepatitis DNA Without Hepatitis DNA 625nm With Hepatitis DNA Without Hepatitis DNA 625nm
Hepatitis B Hepatitis C
Wavelength (nm) Wavelength (nm) Intensity (cts/sec) Intensity (cts/sec)
Fluorescence Emission Spectrum
SLIDE 7
Potential Conditions for Fluorescence Optimization
Silver Salt Solution Reducing Agent
SLIDE 8
Silver Salt
Solution Reducing Agent
Potential Conditions for Fluorescence Optimization
SLIDE 9 Silver Salt
Solution
Reducing Agent
Potential Conditions for Fluorescence Optimization
SLIDE 10 Intensity of Five Trial Solutions
Intensity (cts/sec) NaPO4
Salt Solutions at 20 mM and pH 7
Hepatitis B
SLIDE 11 Intensity (cts/sec) NaPO4 NH4OAc NaOAc NaBr H2O
Hepatitis B
Salt Solutions at 20 mM and pH 7
Intensity of Five Trial Solutions
SLIDE 12
Silver Salt
Solution Reducing Agent
Potential Conditions for Fluorescence Optimization
SLIDE 13 Intensity (cts/sec) 217 Intensity Increase .75 µL Ag 1 µL Ag
Sodium Phosphate at 20mM
More Silver Salt Increases Fluorescent Intensity
Hepatitis B
SLIDE 14 Silver Salt
Solution
Reducing Agent
Potential Conditions for Fluorescence Optimization
SLIDE 15 Decreasing Solution Concentration Increases Fluorescent Intensity
Intensity (cts/sec) NaPO4 20mM Silver Salt = 1µL
Hepatitis B
SLIDE 16 Intensity (cts/sec) NaPO4 20mM NaPO4 30mM NaPO4 10mM
Hepatitis B
Silver Salt = 1µL
Decreasing Solution Concentration Increases Fluorescent Intensity
SLIDE 17 Optimization of Hepatitis C Probe Inconclusive
Hepatitis C
Wavelength (nm) Intensity (cts/sec) Intial Conditions 32 µL of 20 mM NaPO4 1.5 µL of DNA Probe 1.5 µL of Target DNA 0.75 µL of mM Silver Salt
SLIDE 18
Hepatitis DNA Experiment Results
Hepatitis C
SLIDE 19 Conclusion
- Successfully detected DNA sequences.
- Experimented to optimize fluorescence.
- Increased light intensity for Hepatitis B probe.
SLIDE 20 Conclusion
Future Goals:
- Increase effectiveness at lower DNA concentrations
- Continual optimization of syntheses aimed toward
rapid and affordable alternatives for DNA detection.
SLIDE 21
Acknowledgements
Mentor: Jackson Travis Del Bonis-O’Donnell Faculty Advisor: Professor Sumita Pennathur Cooke Bridges Staff: Ofelia Aguirre, Jens Kuhn, Maria Napoli, Chrysafis Andreou, Sarah Maxel, Kori Lay, Spencer Igram, Stephanie Mendes
