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Value of Researching Fluorescent Silver Nanoclusters Rapid and affordable DNA detection http://www.nature.com/scitable/blog/watching-the-detectives/how_will_hiv_affect_human A Novel Approach: Fluorescent Silver Nanoclusters DNA Probe Silver

  1. Value of Researching Fluorescent Silver Nanoclusters Rapid and affordable DNA detection http://www.nature.com/scitable/blog/watching-the-detectives/how_will_hiv_affect_human

  2. A Novel Approach: Fluorescent Silver Nanoclusters DNA Probe Silver Salt and Target DNA Reducing Agent Silver Nanoclusters Image Courtesy of Travis O’Donnell

  3. Detecting DNA with Silver Nanoclusters DNA Probe Silver Salt Reducing Ag nanocluster Agent region Target DNA Image Courtesy of Travis O’Donnell

  4. A Simple DNA Test Hepatitis DNA Silver Salt Reducing Agent Sodium Phosphate No Hepatitis DNA

  5. Fluorescence Emission Spectrum Hepatitis B 625nm With Hepatitis Intensity (cts/sec) DNA Without Hepatitis DNA Wavelength (nm)

  6. Fluorescence Emission Spectrum Hepatitis B Hepatitis C 625nm With With Hepatitis 625nm Intensity (cts/sec) Intensity (cts/sec) Hepatitis DNA DNA Without Without Hepatitis Hepatitis DNA DNA Wavelength (nm) Wavelength (nm)

  7. Potential Conditions for Fluorescence Optimization Silver Salt Reducing Solution Agent

  8. Potential Conditions for Fluorescence Optimization Silver Salt Reducing Solution Agent

  9. Potential Conditions for Fluorescence Optimization Silver Salt Solution Reducing Agent ● Chemical compound

  10. Intensity of Five Trial Solutions Salt Solutions Hepatitis B at 20 mM and pH 7 Intensity (cts/sec) NaPO 4

  11. Intensity of Five Trial Solutions Salt Solutions Hepatitis B at 20 mM and pH 7 Intensity (cts/sec) NaPO 4 NH 4 OAc NaBr NaOAc H 2 O

  12. Potential Conditions for Fluorescence Optimization Silver Salt Reducing Solution Agent

  13. More Silver Salt Increases Fluorescent Intensity Sodium Hepatitis B Phosphate at 20mM Intensity (cts/sec) 217 Intensity Increase .75 µ L Ag 1 µ L Ag

  14. Potential Conditions for Fluorescence Optimization Silver Salt Solution Reducing Agent ● Concentration

  15. Decreasing Solution Concentration Increases Fluorescent Intensity Hepatitis B Intensity (cts/sec) Silver Salt = 1µL NaPO 4 20mM

  16. Decreasing Solution Concentration Increases Fluorescent Intensity Hepatitis B Intensity (cts/sec) Silver Salt = 1µL NaPO 4 NaPO 4 NaPO 4 10mM 20mM 30mM

  17. Optimization of Hepatitis C Probe Inconclusive Hepatitis C Intial Conditions 32 µ L of 20 mM NaPO 4 Intensity (cts/sec) 1.5 µ L of DNA Probe 1.5 µ L of Target DNA 0.75 µ L of mM Silver Salt Wavelength (nm)

  18. Hepatitis DNA Experiment Results Hepatitis C

  19. Conclusion • Successfully detected DNA sequences. • Experimented to optimize fluorescence. • Increased light intensity for Hepatitis B probe.

  20. Conclusion Future Goals: • Increase effectiveness at lower DNA concentrations • Continual optimization of syntheses aimed toward rapid and affordable alternatives for DNA detection.

  21. Acknowledgements Mentor: Jackson Travis Del Bonis-O’Donnell Faculty Advisor: Professor Sumita Pennathur Cooke Bridges Staff: Ofelia Aguirre, Jens Kuhn, Maria Napoli, Chrysafis Andreou, Sarah Maxel, Kori Lay, Spencer Igram, Stephanie Mendes

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