Finding Pneumo HKUST iGEM 2014 TEAM In Intr troduc oduction - - PowerPoint PPT Presentation

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Finding Pneumo HKUST iGEM 2014 TEAM In Intr troduc oduction - - PowerPoint PPT Presentation

Jul 03, 2023 346 likes •736 views

Finding Pneumo HKUST iGEM 2014 TEAM In Intr troduc oduction tion Human n Introduc oducti tion on 2 Conclusi clusion on Pneumo mose senso nsor Riboregul ulator tor Pract ctice ce Bac ackgr ground ound an and d Mo

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Finding Pneumo

HKUST iGEM 2014 TEAM

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SLIDE 2

Introduc

  • ducti

tion

  • n

Pneumo mose senso nsor Riboregul ulator tor Human n Pract ctice ce

Conclusi clusion

  • n

In Intr troduc

  • duction

tion

2

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SLIDE 3

Introduc

  • ducti

tion

  • n

Pneumo mose senso nsor Riboregul ulator tor Human n Pract ctice ce

Conclusi clusion

  • n

Bac ackgr ground

  • und an

and d Mo Motiv tive

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Otitis is Medi dia Pneumonia nia Meningi ngitis is

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SLIDE 4

Introduc

  • ducti

tion

  • n

Pneumo mose senso nsor Riboregul ulator tor Human n Pract ctice ce

Conclusi clusion

  • n

Bac ackgr ground

  • und an

and d Mo Motiv tive

4

1.6 mill llio ion n deaths

hs per ye year ar

40 40-70% 70%

Killed lled or Disabled abled

Slovenia

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SLIDE 5

Introduc

  • ducti

tion

  • n

Pneumo mose senso nsor Riboregul ulator tor Human n Pract ctice ce

Conclusi clusion

  • n

Require laboratory or hospital setting

Urinary antigen test PCR assay X-ray Sputum gram staining

Current Methods

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SLIDE 6

Introduc

  • ducti

tion

  • n

Pneumo mose senso nsor Riboregul ulator tor Human n Pract ctice ce

Conclusi clusion

  • n

Me Mechanism chanism of f Quorum

  • rum Sensing

ensing

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  • S. pneumoniae

DNA uptake and recombination (Competence Stimulating Peptide)

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SLIDE 7

Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

Pneu eumosens mosensor

  • r Over

vervie view

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Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

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Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

Pneu eumosens mosensor

  • r Over

vervie view

9

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SLIDE 10

Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

De Detection ection Mo Module dule

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Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

S.

  • S. pn

pneumon eumoniae iae σx Promot

  • moter

ers s Module dule

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  • E. coli protease
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Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

Functionality of σx

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Scale bar: 5mm | Gain: 4 | Exposure time: 200 ms | Gamma BF: 1 | Gamma GFP: 1

Promoter

σx

Bright Field

GFP

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Pneumosen mosenso sor

Introducti uction Riboregul ulator tor Human n Pract ctice ce Conclusi usion

Relative Promoter Unit Measurement

  • f PcelA and PcomFA

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Relative Promoter Unit Promoter J23101 K1379000 J23101 K1379001 (PcelA) (PcomFA)

σx

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

14 14

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

cis-repressing

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CDS

Ribosome

trans-activating

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

CDS

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TA RBS CR

Ribosome

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

17 17

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

Name of Riboregulator Team Functionality Key1 and Lock1 Berkeley 2005 Yes Key3 and Lock3 Berkeley 2005 Yes

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

Lock ck 1 an and d Key ey 1 Lock ck 3 and and Key ey 3

19 19 Missing TA CR CR&TA Missing TA CR CR&TA

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

20 20

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

DH10B DH5 α BW25113

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Ribor

  • regu

egulat lator

  • r

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Conclusi clusion

  • n

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Relat ativ ive e Promoter

  • moter Unit
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Human an Pract actice ice

Pneumo mose senso nsor Introducti ction Riboregul ulator tor Conclusi usion

Human Practice

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Human an Pract actice ice

Pneumo mose senso nsor Introducti ction Riboregul ulator tor Conclusi usion

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

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Human Practice Projects 2008-2013

3000+ Record Entries

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

Report

Analyze and interpret the data Catch any potential trends

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

31

Types of Projects Done in the Past

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Human an Pract actice ice

Pneumo mose senso nsor Introducti uction Riboregul ulator tor Conclusi usion

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Conclu lusi sion

  • n

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce Rioregul ulator tor

Conclusion

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Conclu lusi sion

  • n

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Riboreg egulato lator

Pneumosensor

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 Characterized PcelA and PcomFA Showed parts from Gram positive bacteria are functional in Gram negative bacteria.

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Conclu lusi sion

  • n

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Riboreg egulato lator

Riboregulator

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Provided additional characterization data for 4 pairs of lock and key Characterized PBAD in 3 different cell strains.

CDS

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Conclu lusi sion

  • n

Pneumo mose senso nsor Introducti uction Human n Pract ctice ce

Riboreg egulato lator

Ackno cknowledgement wledgement

Fundi ding ng Office of the Provost, HKUST Office of the Dean of Science, HKUST Discu cussio ssion n and Technical nical Assistance nce

  • Prof. Siu Chun HUNG
  • Prof. King L. CHOW and his lab members

Reagen ents

  • Prof. Patrick Chiu Yat WOO (HKU)
  • Dr. Don MORRISON

(Université de Toulouse, UPS, Laboratorie de Microbiologie et Génétique Moléculaires) Wellcome Trust Sanger Institute

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Human Practic ice SUSTC-Shenzhen Tec-Monterrey Manchester Cornell

  • Prof. Hon Ming LAM (CUHK)
  • Prof. Tom RICHARD (PSU)
  • Dr. Samuel YU (HKUST)
  • Ms. Christine CHIU (HKUST)
  • Mr. Kevin WONGSO (HKUST)

Independent Schools Foundation Academy

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Finding Pneumo

HKUST iGEM 2014 TEAM