Div. of Bioprocess Engineering School of Biotechnology, KTH Prof Gen - - PowerPoint PPT Presentation

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Feb 15, 2023 358 likes •476 views

Sustainability activities at Div. of Bioprocess Engineering School of Biotechnology, KTH Prof Gen Larsson gen@kth.se THE DEFINITION OF BIOTECHNOLOGY Biotechnology is the integration of natural sciences and engineering in order to achieve the

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Sustainability activities at

Div. of Bioprocess Engineering

School of Biotechnology, KTH

Prof Gen Larsson gen@kth.se

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THE DEFINITION OF BIOTECHNOLOGY

Biotechnology is the integration of natural sciences and engineering in order to achieve the application of

rganisms, cells, parts thereof and molecular analogues for

products and services.

European Federation of Biotechnology, 1980

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THE LIGNOCELLULOSE BIOREFINERY

FUELS ENERGY

Lignocellulose waste SUGARS Thermal decomposition Bioproduction Processing LIGNIN Aromatic compounds Energy Pre‐ treatment

BIOCHEMICALS BIOMATERIAL

CELLULOSE HEMICELLULOSE

non‐BIOTECH PRODUCTS

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HIGH VALUE MATERIALS OR CHEMICALS

EXAMPLE OF APPLICATION AREA :MEDICIN SURGICAL MESH POLYMER: polyhydroxybutyrate (PHB)

PRODUCTS

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Curr Opin Biotechnol 2011, 22:758‐767

PTT HA PLA red: dicarboxylic acids yellow: diamines blue: alkenes, dienes purple: carboxylic acids green: diols PHA´s

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Waste biomass, examples

Sludge from wastewater treatment
Organic Municipal Solid Waste
Fishery waste
e.g. shells from crustaceans
Sugar industry waste
Bagasse/molasses
Forestry residues
wood from forest management
waste streams from pulp and paper industry
Agricultural waste
straws, husks, cobs
Animal farming
e.g. whey from production
f diary products
Clothes recycling
Citrus peels
Chicken feathers
and more…..

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PROBLEM: presence of different sugars

Time (min) Enzymatic saccharification Glucose (mg/g DS) Xylose (mg/g DS) Arabinose (mg/g DS) Galactose (mg/g DS) Total (mg/g DS) After 313 ± 8 198 ± 11 25 ± 3 18 ± 3 555 ± 25

Saha B., Iten L., Cotta M., Wu V. (2005) Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Process Biochem, 40, 3693‐3700. Yields from dilute acid pretreatment at 140°C and enzymatic saccharification of wheat straw (7.83%, w/v DS)

CELL TYPE 2: sequential growth: glucose>arabinose>xylose CELL TYPE 1: cannot grow on 5‐carbons

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Optimal use of the raw material (waste biomass)
Production of monomers for later polymerisation
Production of polymers with a range of different qualities

BIOREFINERY TASK

Unmodified cell Designed cell

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REMOVAL OF PHARMACEUTICALS FROM WASTE WATER

SERVICES

Future: consortium for participation in the Baltic see project (EU) Käppalaverket

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TECHNIQUES

Activated carbon
Ozonation
Biofilters

P P P P P

Capture of pharmaceuticals (API´s) on

cells with designed surfaces

Cells are grown to high density