Discordant Tropism Determination for HIV-1 Isolates of CRF01_AE from - - PowerPoint PPT Presentation

▶
discordant tropism determination for hiv 1 isolates of
SMART_READER_LITE
LIVE PREVIEW

Discordant Tropism Determination for HIV-1 Isolates of CRF01_AE from - - PowerPoint PPT Presentation

Apr 04, 2023 154 likes •213 views

Discordant Tropism Determination for HIV-1 Isolates of CRF01_AE from Asia Wataru Sugiura 1 , Sverine Louvel 2 , Nico Pfeifer 3 , Masakazu Matsuda 2 , Yoshiyuki Yokomaku 2 , Rolf Kaiser 4 , Thomas Klimkait 5 rPhenotyping R5-tropic (TAK779)

slide-1
SLIDE 1

Discordant Tropism Determination for HIV-1 Isolates of CRF01_AE from Asia

Wataru Sugiura1, Séverine Louvel2, Nico Pfeifer3, Masakazu Matsuda2, Yoshiyuki Yokomaku2, Rolf Kaiser4, Thomas Klimkait5

slide-2
SLIDE 2

rPhenotyping

R5-tropic (TAK779) X4-tropic (AMD3100)

slide-3
SLIDE 3

Tropism Testing in CRF01_AE

slide-4
SLIDE 4

Summary

  • This study confirms a significant overcalling of CXCR4-tropic HIV-1

for AE by the current standard versions of G2P[coreceptor].

  • The new Geno2pheno-C_NGS-Sanger calls (X4-risk<36%), trained
  • n NGS-data, provides correct R5-calls for these sequences,

reaching excellent agreement with rPhenotyping

  • This study focused on samples with known discordances between

the Geno2pheno methods, -- could reflect an obvious bias. Therefore best-matching cut-offs for CRF01_AE and/or additional adjustments need further refinement.

  • We aim at expanding the AE-data set, particularly by including

specimens from other global regions with available clinical follow-up information.

slide-5
SLIDE 5

Joëlle Bader Martin Däumer Alex Thielen Swiss HIV Cohort Study