Direct toxin detection Mouse bioassay not sensitive enough HPLC - - PowerPoint PPT Presentation

Direct toxin detection

• Mouse bioassay – not sensitive enough
• HPLC (chromatography) – UV:
• ISSC and FDA approved
• OA, DTX1, DTX2
• Not very sensitive
• Expensive
• Training required
• Slow

BIGELOW.ORG

ASP Setup

Autosampler Column Compartment UV Detector Quaternary Pump Mobile Phase

• LC‐MS/MS
• More sensitive – also allows ‘routine’ seawater analysis
• More expensive
• More training
• Research tool
• Might be useful for

monitoring multiple toxins

Direct toxin detection

Direct toxin detection

Screening approaches

• Immuno‐approaches:

‐ Lateral flow ‐ +/‐ no equipment (Scotia kits) ‐ ELISA: colour change / fluorescence requires some equipment generally

• Physiological approach:

‐ Receptor binding assays: radioistope detection measures ‘total’ toxicity

• Portable approaches:

‐ Lateral flow ‐ + (Scotia kits) ‐ Surface plasmon resonance

Direct toxin detection