Developing a urine test for bladder cancer Prof. Guro E. Lind - - PowerPoint PPT Presentation

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Jan 25, 2023 30 likes •190 views

Centre for Cancer Biomedicine Photo: Jarle Bruun Photo: Jarle Bruun Developing a urine test for bladder cancer Prof. Guro E. Lind Cancer Biomedicine Dept of Molecular Oncology University of Oslo Department of Cancer Prevention Oslo

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Photo: Jarle Bruun

Prof. Guro E. Lind

Dept of Molecular Oncology Cancer Biomedicine

University of Oslo

Developing a urine test for bladder cancer

Photo: Jarle Bruun

Oslo Life Science 2017

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Bladder cancer

Incidence Europe: ~151,000

Department of Molecular Oncology Institute for Cancer Research

~118,000 men ~33,000 women

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Monitored by cystoscopy

Picture from www.onehealthcare.co.uk

Department of Molecular Oncology Institute for Cancer Research

Need for non-invasive test

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Urine contains cancer cells

Department of Molecular Oncology Institute for Cancer Research

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Urine contains cancer cells

Department of Molecular Oncology Institute for Cancer Research

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Urine-based non-invasive test

Costa et al, Clinical Cancer Research, 2010

Department of Molecular Oncology Institute for Cancer Research

Photo: T. Heiestad

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine Biomarker panels increase robustness

Department of Molecular Oncology Institute for Cancer Research

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Prostate cancer n=4 Kidney cancer n=4 Bladder cancer n=8

Department of Molecular Oncology Institute for Cancer Research

Discovery phase

In vitro models: Cancer cell lines

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

3 million positions analyzed

https://www.flickr.com/photos/bigsmiling/15165096671

Department of Molecular Oncology Institute for Cancer Research

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Department of Molecular Oncology Institute for Cancer Research

A Tiling region 1 1 Tiling region 4 4 Tiling region 8 8 Tiling region 9 9 Tiling region 6 6 Tiling region 2 2 Tiling region 3 3 Tiling region 5 5 Tiling region 7 7

Unbiased analyses

B

CG......CG..CG..........CG...CG..........CGCGCG..................CG..CG...CG...CG..........CG.........CG....CG

Genomic DNA sequence 1000 bp 200 bp

Tiling region 10 10

100 bp 100 bp

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Candidate biomarker

Department of Molecular Oncology Institute for Cancer Research

Bladder Prostate & Kidney

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Department of Molecular Oncology Institute for Cancer Research

Bladder Prostate & Kidney

Candidate biomarker

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine Validation in cancers and controls

Department of Molecular Oncology Institute for Cancer Research

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Urine samples

Bladder cancer Healthy controls Prostate cancer Kidney cancer

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Monitoring using urine

Department of Molecular Oncology Institute for Cancer Research

Diagnosis Surgery Biomarker level urine Recurrence Threshold?

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Oslo University Hospital Department of Cancer Prevention Institute for Cancer Research Radiumhospitalet

Centre for Cancer Biomedicine

Department of Molecular Oncology Institute for Cancer Research

Acknowledgement

Genome biology group by Rolf I Skotheim Genetics group by Ragnhild A. Lothe Epigenetics group by Guro E. Lind

Portuguese Oncology Institute

Carmen Jeronimo Manuel Teixeira Rui Henrique Vera Costa

Oslo University Hospital department of urology

Rolf Wahlqvist Anne Klara Sørbø Anne Grethe Johansen Heidi Pharo Kim Andresen Hilde Honne Marcin Wojewodzic Marine Jeanmougin Eirill Ager-Wick (alumni)