Developing a urine test for bladder cancer Prof. Guro E. Lind - PowerPoint PPT Presentation

Centre for Cancer Biomedicine Photo: Jarle Bruun Photo: Jarle Bruun Developing a urine test for bladder cancer Prof. Guro E. Lind Cancer Biomedicine Dept of Molecular Oncology University of Oslo Department of Cancer Prevention Oslo University Hospital Institute for Cancer Research Radiumhospitalet Oslo Life Science 2017

Bladder cancer Centre for Cancer Biomedicine Incidence Europe: ~151,000 ~118,000 men ~33,000 women Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Monitored by cystoscopy Centre for Cancer Biomedicine Need for non-invasive test Picture from www.onehealthcare.co.uk Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Urine contains cancer cells Centre for Cancer Biomedicine Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Urine contains cancer cells Centre for Cancer Biomedicine Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Urine-based non-invasive test Centre for Cancer Biomedicine Photo: T. Heiestad Costa et al, Clinical Cancer Research, 2010 Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Cancer Biomedicine Biomarker panels increase robustness Centre for Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Discovery phase Centre for Cancer Biomedicine In vitro models: Cancer cell lines Bladder cancer n=8 Kidney cancer Prostate cancer n=4 n=4 Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

3 million positions analyzed Centre for Cancer Biomedicine https://www.flickr.com/photos/bigsmiling/15165096671 Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Centre for Unbiased analyses Cancer Biomedicine Genomic DNA sequence CG......CG..CG..........CG...CG..........CGCGCG..................CG..CG...CG...CG..........CG.........CG....CG 1000 bp 200 bp A Tiling region 1 B 1 Tiling region 2 2 3 Tiling region 3 Tiling region 4 4 Tiling region 5 5 6 Tiling region 6 Tiling region 7 7 Tiling region 8 8 9 Tiling region 9 Tiling region 10 10 100 bp 100 bp Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Candidate biomarker Centre for Cancer Biomedicine Bladder Prostate & Kidney Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Candidate biomarker Centre for Cancer Biomedicine Bladder Prostate & Kidney Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Cancer Biomedicine Validation in cancers and controls Centre for Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Urine samples Centre for Cancer Biomedicine Bladder Healthy cancer controls Prostate Kidney cancer cancer Department of Cancer Prevention Oslo University Hospital Institute for Cancer Research Radiumhospitalet

Monitoring using urine Centre for Cancer Biomedicine Diagnosis Recurrence Surgery Biomarker level urine Threshold? Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet

Acknowledgement Centre for Cancer Biomedicine Oslo University Hospital department of urology Rolf Wahlqvist Portuguese Oncology Anne Klara Sørbø Institute Anne Grethe Johansen Carmen Jeronimo Manuel Teixeira Rui Henrique Vera Costa Genome biology group Heidi Pharo by Rolf I Skotheim Kim Andresen Hilde Honne Marcin Wojewodzic Epigenetics group Genetics group Marine Jeanmougin by Guro E. Lind by Ragnhild A. Lothe Eirill Ager-Wick (alumni) Department of Cancer Prevention Oslo University Hospital Department of Molecular Oncology Institute for Cancer Research Institute for Cancer Research Radiumhospitalet