Complete Portfolio of Biopharma Solutions Sample Sample Data - - PowerPoint PPT Presentation

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Complete Portfolio of Biopharma Solutions Sample Sample Data - - PowerPoint PPT Presentation

Mar 20, 2024 154 likes •391 views

Complete Portfolio of Biopharma Solutions Sample Sample Data Preparation Analysis Interpretation Unregulated Regulated UltiMate + TSQ Vantage Regulated quantitation of small molecules, peptides, and biologics Highest sensitivity,

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Complete Portfolio of Biopharma Solutions

Unregulated Regulated

Sample Analysis Sample Preparation Data Interpretation

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UltiMate + TSQ Vantage

  • Highest sensitivity, lowest noise for peptide

mapping of biologics

  • Consistency and ruggedness for high

throughput discovery applications

Regulated quantitation of small molecules, peptides, and biologics

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QuickQuan and QuickCalc

  • Industry’s only fully automated infusion
  • ptimization process
  • HT data acquisition, processing and reporting
  • Multiple calculation modules: PK, Hepatic

Clearance, Permeability

Automate the entire triple quad drug discovery workflow

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MetQuest Software

Automated Quan/Qual Metabolic Screening

  • Integrated workflow for Orbitrap-based

instruments

  • Data acquisition with generic method
  • Unbiased high-resolution full-scan data
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TSQ Module for Watson LIMS

  • Integrated LC-MS/MS control
  • Chromatographic peak integration and

re-integration inside of Watson

  • Single reporting package for LC-MS/MS data
  • Storage of raw data inside of Watson LIMS

Simplifies daily GLP workflow

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Transcend for HTS Applications

  • Improved selectivity with reduced

costs and sample preparation

  • Rapid sample collection with

chromatographic separation

  • Triple quad and HRAM compatible
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Laser Diode Thermal Desorption

  • Rapid compound optimization and sample

analysis

  • Ten 96-well plates in less than 3 hours
  • No additional sample preparation
2.2 2.4 2.6 2.8 Time (min) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 Relative Abundance 1.8 2.0 2.2 2.4 2.6 2.8 Time (min) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 Relative Abundance 2.2 2.4 2.6 2.8 Time (min) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 Relative Abundance 2.2 2.4 2.6 2.8 Time (min) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 Relative Abundance 1.8 2.0 2.2 2.4 2.6 2.8 Time (min) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 Relative Abundance 1.8 2.0 2.2 2.4 2.6 2.8 Time (min) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 Relative Abundance
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UltiMate 3000 MAb Analysis Platform

  • Fully biocompatible
  • Dual ternary gradient pumps
  • Multifunctional autosampler with fraction

collection and sample derivatization

  • Protein purification
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10 Orbitrap, R= 7,500 53.8 fmol 5.38 fmol Minutes Normalized intensity (a) (b) 3,100 4,000 2,200 53.8 fmol m/z (c) 3,100 4,000 2,200 5.38 fmol m/z (d)

Achieve ultimate confidence

Intact Monoclonal Antibody Analysis Using LC-MS

  • Dionex monolithic columns for intact

protein analysis

  • Dionex UltiMate 3000 RSLCnano for

high chromatographic fidelity

  • High mass accuracy, high-resolution

mass spectrometric detection

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deamidation

y6+1 b10-H2O b14+1 y2 y3 b6 y4 b7 y5 b8 y6 b9 y7 b10 y8 b11 y9 b12 y10 b13 y11 b14 S G T A S V V C+58 L L N+1 N F Y P R R P Y F N N+1 L L C+58 V V S A T G S

m/z Relative Intensity 0% 20% 40% 60% 80% 100% 250 500 750 1000 1250 1500 1750

MS/MS spectrum

Minutes 5 10 1.5 Intensity ×107 15 20 25 30 35

Lys C MAb Digest Analysis by Capillary LC-MS/MS

  • UHPLC for high

chromatographic fidelity and resolution

  • High-resolution MS for

fast and accurate protein identification

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EASY-nLC 1000 Effortless UHPLC

  • 1000 bar operating pressure
  • Dedicated solution
  • Market-leading ease of use
  • Integrated design, easy maintenance
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EASY-nLC 1000 Xcalibur Control

  • Versatile MS data system
  • Wizard-style method development
  • 21 CFR Part 11 compliance
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EASY-nLC 1000 Ready to run

  • Predefined tubing
  • MS-based QC
  • Instant trouble-free separation
  • Uncompromising performance
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Pinpoint Quantitative Proteomics Software

  • Accelerates targeted protein quantitation in

biological research

  • Facilitates the transition to targeted quantitation
  • Start-to-finish solution for biomarker ID and

verification

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End-to-end Bioanalysis Solutions

  • Best-in-class protein analysis

columns

  • HPLC platforms to increase

throughput and productivity

  • Global leader in high-resolution, accurate

mass MS for ultimate confidence

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Quantitation by MS

HeavyPeptide AQUA Standards

For relative and absolute quantitation of proteins in Proteomics and Biopharma

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Better MS Sample Prep

Pierce Graphite Spin Columns

Purify and concentrate hydrophilic peptides in <10 minutes

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Detergent Removal Products

Better sample prep means better results

New 96-well spin plates for high-throughput, or spin columns for 10µl-1mL samples

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Capture and Enrich Kinases

Enrichment kits with novel ATP- and ADP-based probes

Covalently modify active sites

  • f kinases, chaperones and

metabolic enzymes