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CambridgeSoft Introduction to BioAssay BioAssay Protocols

CambridgeSoft Corp. Cambridge, MA USA

Protocols

USA www.cambridgesoft.com

Kate Sydney Kate Sydney Manager, Marketing Communications

Presentation Outline

 What is BioAssay?  How do I make BioAssay

work?

 What is a protocol?

What are the data sources

 What are the data sources

for BioAssay?

 Demo  How do I get access to

BioAssay? Wh t t f li t

 What sorts of licenses to

BioAssay does CambridgeSoft sell?

W hat is BioAssay?

Import and storage of assay data Automated handling of kfl assay workflows G hi l d h Graphical outputs and heat maps Mathematical manipulation and analysis of assay information

How do I make BioAssay W ork?

Fi d iti

Example % Inhibition Experiment Workflow

Accept data from plate reader Prompt for plate ID, notebook #, scientist name Use pre- defined plate format “80 compound screen” Find positive and negative control wells and calculate Calculate % inhibition against raw data in wells Graph % Inhibition against Compound ID name screen average ID

W hat is a protocol?

A hierarchical t ti f representation of your experiment workflow

Another w ay of looking at it

Plate

fx Pos/Neg Ctrl Avg Y = Average (all wells with Well ID = empty or pos control)

Graph %Inhibition Graph %Inhibition

Well

fx %Inhibition

control)

fx %Inhibition Y = 100 * ((raw value – negative control average)/(positive control average – negative control average)

Data sources for BioAssay

Plate Setup Data

• Defines what type of plate you are working with. (Ex: 96-well,

yp p y g ( 384-well)

Well Setup Data

• Defines what substance is in each well of the plate. (Ex:

Empty, compound, negative control, positive control)

Protocol Setup Data Protocol Setup Data

• Defines what actions and calculations you want to take place

upon import of data

Raw Data

• Can be virtually anything and can be in plate block format,

columnar data or a custom format that you can define columnar data or a custom format that you can define

BIOASSAY DE MO

How do I get access to BioAssay?

W hat sorts of licenses to BioAssay does CambridgeSoft Sell?

Enterprise software capabilities p p