CambridgeSoft Introduction to BioAssay BioAssay Protocols - PowerPoint PPT Presentation

E-Notebook CambridgeSoft Introduction to BioAssay BioAssay Protocols Protocols CambridgeSoft Corp. Cambridge, MA USA USA www.cambridgesoft.com Kate Sydney Kate Sydney Manager, Marketing Communications

Presentation Outline  What is BioAssay?  How do I make BioAssay work?  What is a protocol?  What are the data sources What are the data sources for BioAssay?  Demo  How do I get access to BioAssay?  What sorts of licenses to Wh t t f li t BioAssay does CambridgeSoft sell?

W hat is BioAssay? Import and storage of assay data Automated handling of assay workflows kfl G Graphical outputs and heat hi l d h maps Mathematical manipulation and analysis of assay information

How do I make BioAssay W ork? Example % Inhibition Experiment Workflow Fi d Find positive iti Prompt for Use pre- Graph % and negative Calculate % Accept data plate ID, defined plate Inhibition control wells inhibition from plate notebook #, format “80 against and against raw reader scientist compound Compound calculate data in wells name name screen” screen ID ID average

W hat is a protocol? A hierarchical representation of t ti f your experiment workflow

Another w ay of looking at it Plate fx Pos/Neg Ctrl Avg Y = Average (all wells with Graph %Inhibition Graph %Inhibition Well ID = empty or pos control) control) Well fx %Inhibition fx %Inhibition Y = 100 * ((raw value – negative control average)/(positive control average – negative control average)

Data sources for BioAssay Plate Setup Data • Defines what type of plate you are working with. (Ex: 96-well, yp p y g ( 384-well) Well Setup Data • Defines what substance is in each well of the plate. (Ex: Empty, compound, negative control, positive control) Protocol Setup Data Protocol Setup Data • Defines what actions and calculations you want to take place upon import of data Raw Data • Can be virtually anything and can be in plate block format, columnar data or a custom format that you can define columnar data or a custom format that you can define

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How do I get access to BioAssay?

W hat sorts of licenses to BioAssay does CambridgeSoft Sell? Enterprise software capabilities p p